2000
DOI: 10.1017/s1355838200991635
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Structural requirement for the two-step dimerization of human immunodeficiency virus type 1 genome

Abstract: Generation of RNA dimeric form of the human immunodeficiency virus type 1 (HIV-1) genome is crucial for viral replication. The dimerization initiation site (DIS) has been identified as a primary sequence that can form a stem-loop structure with a self-complementary sequence in the loop and a bulge in the stem. It has been reported that HIV-1 RNA fragments containing the DIS form two types of dimers, loose dimers and tight dimers. The loose dimers are spontaneously generated at the physiological temperature and… Show more

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Cited by 103 publications
(108 citation statements)
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“…The results with the substitution and deletion mutants prove that the presence of a 6-mer palindrome within the DIS loop is critical for in vitro dimerization, which is consistent with several HIV-1 studies (14,17,35,36). The dimerization efficiency of the palindrome variants GCGCGC, GUGCAC, and GUCGAC is quite similar to that of the wild type GGUACC, suggesting that there is no strict palindrome sequence requirement for dimer formation.…”
Section: Fig 8 Phylogenetic Analysis Of the Dis Hairpin In Hiv-siv supporting
confidence: 85%
“…The results with the substitution and deletion mutants prove that the presence of a 6-mer palindrome within the DIS loop is critical for in vitro dimerization, which is consistent with several HIV-1 studies (14,17,35,36). The dimerization efficiency of the palindrome variants GCGCGC, GUGCAC, and GUCGAC is quite similar to that of the wild type GGUACC, suggesting that there is no strict palindrome sequence requirement for dimer formation.…”
Section: Fig 8 Phylogenetic Analysis Of the Dis Hairpin In Hiv-siv supporting
confidence: 85%
“…We propose that NCp7-binding can destabilize the kissing-loop dimer by inhibiting the coaxial stacking. In addition, we find that the extended-duplex dimer becomes energetically more favorable as the temperature increases, which is also consistent with the experiment (Muriaux et al 1996b;Takahashi et al 2000).…”
Section: Introductionsupporting
confidence: 91%
“…The sample was heated to 90°C for 3 min and slowly cooled to room temperature at an RNA concentration of ϳ7 M, then concentrated to 0.75 mM. The formation of the linear isomer of the SL1 dimer was verified by PAGE as described (16). The NMR sample had RNA at concentration of 0.75 mM in 300 l of 10 mM potassium phosphate buffer with 250 mM NaCl and 0.1 mM MgCl 2 at pH 6.4, either in D 2 O or in a 90/10% H 2 O/D 2 O mixture.…”
Section: Methodsmentioning
confidence: 99%