Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment

Li, Mo; Li, Tong; Hu, Xinyu; Ren, Guangyu; Zhang, He-Nan; Wang, Zijian; Teng, Zhengrong; Wu, Rina; Wu, Junrui

doi:10.1016/j.ijbiomac.2021.05.108

Cited by 42 publications

(17 citation statements)

References 48 publications

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“…Among these methods, HEO had the largest DP. It was reported that Li et al used hot water, ultrasound-assisted, and enzyme-assisted methods to prepare mulberry fruit polysaccharides, and the molecular weight of the hot water extract was the largest, which was consistent with the results of this study [ 34 ].…”

Section: Resultssupporting

confidence: 91%

“…The presence of uronic acid induces saccharides to be negatively charged [ 39 ]. A higher uronic acid content will lead to a greater absolute value of the Zeta potential, which in turn may result in stronger antioxidant activity [ 34 ]. In addition, some studies have shown that protein content was one of the factors that affected the antioxidant capacity of oligosaccharides, as -NH 2 can absorb hydrogen ions in the solution to form-NH 3 + and reacts with the radicals [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

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Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

et al. 2021

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Evodia lepta (E. lepta) is a traditional Chinese herbal medicine with various biological activities. One of the active components of this widely used medicinal plant is believed to be an oligosaccharide. The extraction yields, structural characteristics, antioxidant, and antitumor activities of four oligosaccharide extracts obtained by hot water extraction (HEO), ultrasound-assisted extraction (UEO), enzyme-assisted (EEO), and microwave-assisted extraction (MEO) were investigated. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS), X-ray diffraction (XRD), and Scanning electron microscopy (SEM) results indicated that the extraction methods had a difference on the molecular mass distribution, structure, and morphology of the EOs. In addition, HEO and MEO showed strong antioxidant activities, which might be related to their uronic acid and protein contents. More interestingly, MEO was more active toward MDA-MB-231 cells compared to other cells, and cell growth inhibition was proposed to occur through apoptosis. Overall, microwave-assisted extraction is a promising technique for the extraction of high quality EO.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

et al. 2021

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“…In a number of works, the same ratio of pectin and DPPH radical was used as in our study, where the ratio of pectin to radical was 1:1 ( v/v ) at a radical concentration of 0.2–0.3 mM. A comparison of data from these studies indicates that the activity of fireweed pectins (IC 50 —0.05 and 0.109 mg/mL) seems to be comparable to that of polysaccharides from Lonicera japonica Thunb (0.01–2.00 mg/mL) [ 39 ], mulberry fruits (0.17–0.32 mg/mL) [ 40 ] and Acanthopanax senticosus leaves (~0.3 mg/mL) [ 41 ]. It should be noted that some other pectins demonstrate DPPH scavenging activity at much higher concentrations.…”

Section: Resultsmentioning

confidence: 99%

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Popov¹,

Смирнов²,

Kvashninova³

et al. 2021

Molecules

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The aim of this study was to isolate pectins with antioxidant activity from the leaves of Epilobium angustifolium L. Two pectins, EA-4.0 and EA-0.8, with galacturonic acid contents of 88 and 91% were isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 4.0 and 0.8, respectively. EA-4.0 and EA-0.8 were found to scavenge the DPPH radical in a concentration-dependent manner at 17–133 μg/mL, whereas commercial apple pectin scavenged at 0.5–2 mg/mL. The antioxidant activity of EA-4.0 was the highest and exceeded the activity of EA-0.8 and a commercial apple pectin by 2 and 39 times (IC50—0.050, 0.109 and 1.961 mg/mL), respectively. Pectins EA-4.0 and EA-0.8 were found to possess superoxide radical scavenging activity, with IC50s equal to 0.27 and 0.97 mg/mL, respectively. Correlation analysis of the composition and activity of 32 polysaccharide fractions obtained by enzyme hydrolysis and anionic exchange chromatography revealed that the antioxidant capacity of fireweed pectins is mainly due to phenolics and is partially associated with xylogalacturonan chains. The data obtained demonstrate that pectic polysaccharides appeared to be bioactive components of fireweed leaves with high antioxidant activity, which depend on pH at their extraction.

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“…These layers were difficult to extract with water, so there were few studies on cell wall polysaccharides. Many extraction techniques were used to release cell wall polysaccharides such as acid extraction [ 19 ], alkali extraction [ 20 ], superfine grinding extraction [ 21 ], ultrasound-assisted extraction [ 22 ] and enzyme-assisted extraction [ 23 ]. Previous reports showed that acid-alkali and superfine grinding extraction can significantly improve the yields of polysaccharides [ 24 , 25 ], thus they were widely used for the extraction of cell wall polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

Liu

Zhang

et al. 2022

Polymers

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The cell wall polysaccharides were extracted from Sparassis latifolia fruit bodies by acid–alkali and superfine-grinding assisted methods, and the chemical characterization and in vitro immunity activities of these polysaccharide fractions were studied and compared. Results showed that superfine-grinding assisted extraction exhibited the highest yield of polysaccharides (SP, 20.80%) and low β-glucan content (19.35%) compared with alkaline extracts. The results revealed that the 20% ethanol precipitated fraction (20E) from SP was mainly composed of β-(1→3)-glucan and α-(1→4)-glucan. With the increase of ethanol precipitation, the fractions (30E, 40E, 50E) were identified as α-(1→4)-glucan with different molecular weights and conformations. Cell wall polysaccharides extracted through NaOH (NSP) and KOH (KSP) extraction had similar yields with 8.90% and 8.83%, respectively. Structural analysis indicated that the purified fraction from KSP (KSP-30E) was a β-(1→3)-glucan backbone branched with β-(1→6)-Glcp, while the purified fraction from NSP (NSP-30E) mainly contained β-(1→3)-glucan with a small number of α-linked-Glcp. The two fractions both exhibited rigid chain conformation in aqueous solutions. All polysaccharide fractions exerted the activity of activating Dectin-1 receptor in vitro, and the KSP-30E mainly identified as β-(1→3)-glucan with the terminal group via 1→6-linkage attached at every third residue exhibited a stronger enhancing effect than other fractions. Results suggested that KOH extraction could be efficient for the preparation of bioactive β-(1→3, 1→6)-glucan as a food ingredient.

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Structural, rheological properties and antioxidant activities of polysaccharides from mulberry fruits (Murus alba L.) based on different extraction techniques with superfine grinding pretreatment

Cited by 42 publications

References 48 publications

Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

Characterization, Antioxidant and Antitumor Activities of Oligosaccharides Isolated from Evodia lepta (Spreng) Merr. by Different Extraction Methods

Isolation, Chemical Characterization and Antioxidant Activity of Pectic Polysaccharides of Fireweed (Epilobium angustifolium L.)

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

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