The advantages of using prestressed carbon fiber reinforced polymer (CFRP) for strengthening and retrofitting structures have been reported in recent years. In this regard, most of the studies on prestressed CFRP technique have been carried out in the laboratory test with small-scale and no damage (reinforced concrete) RC beam. However, the real structures that need to be retrofitted in service are often degraded or damaged due to early cracking. This paper aims at studying the effect of prestressed CFRP method on full-scale and damaged RC beams. The damaged levels of four full-scale damaged hollow RC beams taken from an old bridge were evaluated. One damaged beam was tested to check the residual capacity, and the other three were strengthened with prestressed composite strengthened CFRP and steel-carbon fiber reinforced polymer (SCFRP). The flexural behavior of non-strengthened and prestressed strengthened beams was investigated. During the experiments, the failure modes, deflection, yield and ultimate load, strains of concrete, steel reinforcements, and SCFRP were measured and analyzed. The results showed that the stiffness at the elastic stage was increased by 64.9%, 66.9%, and 67.1% after strengthened by SCFRP with 30%, 40%, and 60% prestressing level. Moreover, the ultimate load of damaged hollow RC beams were improved by 19.53%, 21.82%, and 31.9%, respectively. The flexural behavior of the severely damaged RC beam with strength reduction coefficient of 0.65 can be recovered after being strengthened by SCFRP with 40% prestressing levels. Meanwhile, SCFRP-concrete interface debonding failure occurred when the prestressing level exceed 60%, and the characteristics of brittle failure became more evident with increased prestressing level of the SCFRP.