Structural studies of an acidic galactorhamnan from the O2 reference strain (C.D.C. 868-57) of serratia marcescens

Oxley, David; Wilkinson, Stephen G.

doi:10.1016/0008-6215(88)84094-1

Carbohydrate Research

1988

DOI: 10.1016/0008-6215(88)84094-1

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Structural studies of an acidic galactorhamnan from the O2 reference strain (C.D.C. 868-57) of serratia marcescens

David Oxley¹,

Stephen G. Wilkinson²

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1989

1997

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Cited by 13 publications

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“…Watersoluble polymeric products released by mild hydrolysis of the products (aqueous 1% acetic acid, 100ЊC, 2.25 h) were isolated by chromatography on Sephadex G-50 and were fractionated by stepwise elution from DEAE-Sepharose CL-6B. By this general procedure, acidic polymers were isolated from strains O1 (14), O2 (19), O3 (21), O4 (18), O6 (7), O13 (17), O14 (7), O15 (20), O18 (23), O22 (24), O23 (22), and IP421 (17), and their structures were determined. Other acidic polymers (which were incompletely characterized) were isolated from the O5, O7, O11, O12, O28, CDC 1783-57, and S86/3049 strains.…”

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confidence: 99%

Identification of capsular antigens in Serratia marcescens

1997

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Previous studies with 31 strains of Serratia marcescens, including 28 reference O-serotype strains, have indicated that 19 of them have an acidic polysaccharide which copurifies with lipopolysaccharide during phenol-water extraction. Polysaccharide in crude extracts from 18 of the 19 strains was precipitated with Cetavlon (hexadecyltrimethyl ammonium bromide), and capsules were demonstrated around these 18 strains by Indian ink exclusion zones. Capsule-antibody binding by the Quellung reaction suggested that the acidic polysaccharide formed the capsule around the bacterial cells. Anticapsular (anti-K) antibody was detected in reference O antisera which had been prepared against boiled whole cells. Cross-titration and absorption studies revealed 14 different K antigens among these strains.Serratia marcescens is an infrequent cause of infection in hospitals, but outbreaks do occur and may involve large numbers of patients as well as environmental contamination (10). Consequently, it is important to be able to identify different strains of this species for epidemiological studies, and serotyping has often been used for this purpose. The O serotype of a strain is determined by its O antigens; these antigens correlate with differences in chemical structure of the oligosaccharide repeating units of lipopolysaccharides (LPSs). K serotypes (or K antigens) are invariably capsular, and many are determined by acidic oligosaccharide repeating units distinct from those of LPS. The capsule is a highly aqueous layer that surrounds the cell and that is distinct from exo-or slime polysaccharides. To date, only O serotypes have been proposed for S. marcescens, although it is clear from previous studies that not all agglutination reactions are attributable to LPS O antigens (12). Chemical analysis of purified LPS obtained from S. marcescens by phenol-water extraction has revealed that many of the Oserotype strains of this species have two polymers with different repeating units: a neutral and an acidic polysaccharide (29). Immunoblotting of crude LPS-containing extracts with absorbed rabbit O-type antisera has suggested that the neutral polysaccharides correspond to the O antigen (11,12), but this technique failed to identify the antigenic nature of the acidic polysaccharides. We therefore set out to detect the presence of capsules in the O-serotype strains of S. marcescens as a prerequisite for the establishment of a chemically and serologically defined serotyping scheme. 60 AUCKEN ET AL.

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Identification of capsular antigens in Serratia marcescens

1997

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Structural studies of an acidic galactoglucomannan from the O3 reference strain (C.D.C. 863-57) of Serratia marcescens

Oxley

Wilkinson

1989

Carbohydrate Research

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Structural studies of a neutral polymer (the putative O10 antigen) isolated from the lipopolysaccharide of Serratia marcescens strain C.D.C. 1287-54 (O10:H8)

Oxley

Wilkinson

1989

Carbohydrate Research

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Structural studies of an acidic galactorhamnan from the O2 reference strain (C.D.C. 868-57) of serratia marcescens

Cited by 13 publications

References 10 publications

Identification of capsular antigens in Serratia marcescens

Identification of capsular antigens in Serratia marcescens

Structural studies of an acidic galactoglucomannan from the O3 reference strain (C.D.C. 863-57) of Serratia marcescens

Structural studies of a neutral polymer (the putative O10 antigen) isolated from the lipopolysaccharide of Serratia marcescens strain C.D.C. 1287-54 (O10:H8)

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