Structural studies of an immunoenhancing glucan of an ectomycorrhizal fungus Ramaria botrytis

Bhanja, Sunil K.; Rout, Dilip; Patra, Pradip; Nandan, Chanchal K.; Behera, Birendra; Maiti, Tapas K.; Islam, Syed S.

doi:10.1016/j.carres.2013.03.023

Cited by 30 publications

(11 citation statements)

References 30 publications

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“…7 The structural features of polysaccharides obtained from mushrooms consist of bor aor both aand blinked glucan backbones displaying different patterns and degrees of branching. 8,9 In living organisms, oxidation is a fundamental process for the production/conversion of energy for the maintenance of biological processes. However, the uncontrolled production of reactive oxygen species (ROS) is responsible for several diseases such as cancer, rheumatoid arthritis, atherosclerosis, coronary heart disorder, etc.…”

Section: Introductionmentioning

confidence: 99%

Chemical structure and biological properties of a polysaccharide isolated from Pleurotus sajor-caju

Seedevi

Ganesan

Mohan³

et al. 2019

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Chemical structure and biological properties of a polysaccharide isolated from Pleurotus sajor-caju

Seedevi

Ganesan

Mohan³

et al. 2019

RSC Adv.

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“…A glucan consisting of (1→6)-linked-β-d glucopyranosyl residues as backbone, branched at O-3 position with a (1→3)-linked-β-d-glucopyranosyl unit and a non-reducing end β-d-glucopyranosyl residue has been purified by the same research group. This glucan showed immunostimulating activity on RAW 264.7, a murine macrophage cell line, by nitric oxide production [198]. Moreover, polysaccharides from R. botrytis showed potent antioxidant activities [199].…”

Section: Ramaria Botrytis (Pers) Bourdotmentioning

confidence: 97%

“…Polysaccharide [199], Extract [202][203][204] -A novel ubiquitin-like protein [196] Glucan (immunostimulating activity) [198] Rugiboletus extremiorientalis (Lj.N. Vassiljeva) G. Wu & Zhu L. Yang ---Leccinine A (84) (protective activity against endoplasmic reticulum stress-dependent cell death [205] and plant growth regulatory activity [206]).…”

Section: Thelephora Ganbajun M Zangmentioning

confidence: 99%

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

et al. 2020

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Mushrooms have a long history of uses for their medicinal and nutritional properties. They have been consumed by people for thousands of years. Edible mushrooms are collected in the wild or cultivated worldwide. Recently, mushroom extracts and their secondary metabolites have acquired considerable attention due to their biological effects, which include antioxidant, antimicrobial, anti-cancer, anti-inflammatory, anti-obesity, and immunomodulatory activities. Thus, in addition to phytochemists, nutritionists and consumers are now deeply interested in the phytochemical constituents of mushrooms, which provide beneficial effects to humans in terms of health promotion and reduction of disease-related risks. In recent years, scientific reports on the nutritional, phytochemical and pharmacological properties of mushroom have been overwhelming. However, the bioactive compounds and biological properties of wild edible mushrooms growing in Southeast Asian countries have been rarely described. In this review, the bioactive compounds isolated from 25 selected wild edible mushrooms growing in Southeast Asia have been reviewed, together with their biological activities. Phytoconstituents with antioxidant and antimicrobial activities have been highlighted. Several evidences indicate that mushrooms are good sources for natural antioxidants and antimicrobial agents

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“…Ramaria botrytis , a kind of edible mushrooms found all over the world especially in mountains of eastern Asia, Europe and North America, is well known for its peculiar flavor like chicken breast meat taste and a fruity scent (Bhanja et al 2013 ). The fruiting bodies of R. botrytis have high medicinal values.…”

Section: Introductionmentioning

confidence: 99%

“…Components like minerals and amino acids (Chen and Xi 2001 ), small molecule compounds, e.g. phenolic acids and ceramide (Barros et al 2009 ; Yaoita et al 2007 ) and polysaccharides (Bhanja et al 2014 ; Li et al 2012 ; Wang et al 2012 ), and bioactivities like antimicrobial activity (Alves et al 2012 ), anti-oxidative activity (Barros et al 2009 ; Kim and Lee 2003 ), anti-proliferation activity (Chung 1979 ), protective effect on liver injury (Kim et al 2003 ; Kim and Lee 2003 ), and immunoenhancing activity (Bhanja et al 2013 ) have been reported on the mushroom. However, no information about purified proteins from R. botrytis was available.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a novel ubiquitin-like antitumour protein with hemagglutinating and deoxyribonuclease activities from the edible mushroom Ramaria botrytis

et al. 2017

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A novel ubiquitin-like antitumour protein (RBUP) was isolated from fruiting bodies of the edible mushroom Ramaria botrytis. The protein was isolated with a purification protocol involving ion exchange chromatography on DEAE-Sepharose fast flow and gel filtration on Sephadex G-75. SDS-PAGE, Native-PAGE and ultracentrifugation analysis disclosed that RBUP was a monomeric protein with a molecular weight of 18.5 kDa. ESI–MS/MS demonstrated that it shared 69% amino acid sequence similarity with Coprinellus congregates ubiquitin (gi|136667). The protein exhibiting strong anticancer activity towards A549 cells. Analysis by employing AO/EB staining and Annexin V-FITC/PI detection indicated that the cytotoxic effect of RBUP was mediated through induction of apoptosis. Furthermore, RBUP displayed hemagglutinating and deoxyribonuclease activities. A temperature of 40 °C and pH of 7.0 were required for optimal DNase activity. Therefore, it was estimated that RBUP exerted its antitumour effect by inducing apoptosis, and its hemagglutinating and DNase activities were also thought to participate in this effect. These results demonstrated that RBUP was a multifunctional protein with potential medicinal applications.

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Structural studies of an immunoenhancing glucan of an ectomycorrhizal fungus Ramaria botrytis

Cited by 30 publications

References 30 publications

Chemical structure and biological properties of a polysaccharide isolated from Pleurotus sajor-caju

Chemical structure and biological properties of a polysaccharide isolated from Pleurotus sajor-caju

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

Purification and characterization of a novel ubiquitin-like antitumour protein with hemagglutinating and deoxyribonuclease activities from the edible mushroom Ramaria botrytis

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