Structural studies of avian myeloblastosis virus: Presence of transfer RNA in core component

Stromberg, Kurt; Litwack, Marcia

doi:10.1016/0005-2787(73)90005-1

Cited by 4 publications

(1 citation statement)

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“…Each particle was assumed to contain a single RNA genome of 107 daltons. About 30% of the RNA content of the virion is 4S RNA and about 20% of the RNA content of the core component is 4S RNA (31). Consequently, this genome represents 70% of the RNA in the virion and 80% of the RNA in the core.…”

Section: Pagementioning

confidence: 99%

Structural Studies of Avian Myeloblastosis Virus: Comparison of Polypeptides in Virion and Core Component by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

Stromberg

Hurley

Davis

et al. 1974

J Virol

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Two different systems of dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in separate laboratories detected analogous patterns of dye bands in virions of avian myeloblastosis virus (AMV). At least 11 of the dye bands co-migrated with the major polypeptides reported in Rous sarcoma virus. Particles with the morphology of the AMV core component, obtained after exposure of AMV to the nonionic surfactant Sterox SL, contained major polypeptides p12, p27, p60, p64, p91, and p98. The polypeptide p12 has been previously shown to be the major constituent of the inner ribonucleoprotein (RNP) of the AMV core, and has been designated p12(N). Two RNP polypeptides, p64 and p91, co-electrophoresed with purified AMV DNA polymerase and have now been designated p64(P) and p91(P). The polypeptide p27 has been identified as a probable constituent of the core shell, and has accordingly now been designated p27(C). In comparison to virions of AMV, the AMV core component contained a greatly reduced amount of polypeptide p15 and appeared to lack a major polypeptide, p19. Consequently, these polypeptides may be associated either with the exterior of the core shell or the interior of the viral envelope. Glycopeptides were not detected in AMV cores, in agreement with earlier reports that they reside in external projections from the viral envelope.

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Section: Pagementioning

confidence: 99%