Structural studies of the lamellar to bicontinuous gyroid cubic (QGII) phase transitions under limited hydration conditions

Tang, T-Y Dora; Brooks, Nicholas J.; Ces, Oscar; Seddon, John M.; Templer, Richard H.

doi:10.1039/c4sm02724h

Cited by 11 publications

(12 citation statements)

References 38 publications

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“…2, a-c resembling the inverse of the lamellar-to-cubic phase transition described by Tang and co-workers (Fig. 5 in (40)). This conformational change appears to be driven by the hydrophobic interactions in the system, as the structural rearrangements (from Fig.…”

Section: Human Skin Glycosylceramides Stabilize a Folded Bilayer Structure With Cubic-like Symmetrysupporting

confidence: 59%

Structural Transitions in Ceramide Cubic Phases during Formation of the Human Skin Barrier

Wennberg

Narangifard

Lundborg

et al. 2018

Biophysical Journal

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The stratum corneum is the outermost layer of human skin and the primary barrier toward the environment. The barrier function is maintained by stacked layers of saturated long-chain ceramides, free fatty acids, and cholesterol. This structure is formed through a reorganization of glycosylceramide-based bilayers with cubic-like symmetry into ceramide-based bilayers with stacked lamellar symmetry. The process is accompanied by deglycosylation of glycosylceramides and dehydration of the skin barrier lipid structure. Using coarse-grained molecular dynamics simulation, we show the effects of deglycosylation and dehydration on bilayers of human skin glycosylceramides and ceramides, folded in three dimensions with cubic (gyroid) symmetry. Deglycosylation of glycosylceramides destabilizes the cubic lipid bilayer phase and triggers a cubic-to-lamellar phase transition. Furthermore, subsequent dehydration of the deglycosylated lamellar ceramide system closes the remaining pores between adjacent lipid layers and locally induces a ceramide chain transformation from a hairpin-like to a splayed conformation.

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Section: Human Skin Glycosylceramides Stabilize a Folded Bilayer Structure With Cubic-like Symmetrysupporting

confidence: 59%

Structural Transitions in Ceramide Cubic Phases during Formation of the Human Skin Barrier

Wennberg

Narangifard

Lundborg

et al. 2018

Biophysical Journal

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“…This process should also take place while D and G twins are formed. It has been suggested that bicontinuous phases can be transformed from lamellar phases by forming so-called interlamellar attachments (ILAs) between adjacent bilayers (Squires et al, 2002;Conn et al, 2006;Tang et al, 2015). More recently, direct imaging has also shown that ILAs emerge as open necks between parallel planes (Demurtas et al, 2015).…”

Section: Bcs Twinning As Stacking Faultmentioning

confidence: 99%

Crystal twinning of bicontinuous cubic structures

Han

Fujita

Chen

et al. 2020

IUCrJ

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Bicontinuous cubic structures in soft matter consist of two intertwining labyrinths separated by a partitioning layer. Combining experiments, numerical modelling and techniques in differential geometry, we investigate twinning defects in bicontinuous cubic structures. We first demonstrate that a twin boundary is most likely to occur at a plane that cuts the partitioning layer almost perpendicularly, so that the perturbation caused by twinning remains minimal. This principle can be used as a criterion to identify potential twin boundaries, as demonstrated through detailed investigations of mesoporous silica crystals characterized by diamond and gyroid surfaces. We then discuss that a twin boundary can result from a stacking fault in the arrangement of inter-lamellar attachments at an early stage of structure formation. It is further shown that enhanced curvature fluctuations near the twin boundary would cost energy because of geometrical frustration, which would be eased by a crystal distortion that is experimentally observed.

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“…This would be consistent with the hypothesis that the cell changes its lipid composition to lower the energetic barrier to entering the next phase of the cell cycle [ 16 ]. Furthermore, work on replicating lipid systems with similar dimensions to those observed in nature show that they are sensitive to parameters such as composition and surface charge [ 37 , 52 , 56 ]. Control of these parameters, with respect to cell size, therefore forms an important part of managing the structure of the cell through the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote

Vítová

Lanta

Čížková

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure is important for progress through and perhaps even control of the cell cycle. Lipidomics ( 31 P NMR and MS), proteomics (Western immunoblotting) and transcriptomics (RT-qPCR) techniques were used to profile the lipid fraction and characterise aspects of its metabolism at seven stages of the cell cycle of the model eukaryote, Desmodesmus quadricauda . We found considerable, transient increases in the abundance of phosphatidylethanolamine during the G 1 phase (+35%, ethanolamine phosphate cytidylyltransferase increased 2·5×) and phosphatidylglycerol (+100%, phosphatidylglycerol synthase increased 22×) over the G 1 /pre-replication phase boundary. The relative abundance of phosphatidylcholine fell by ~35% during the G 1 . N -Methyl transferases for the conversion of phosphatidylethanolamine into phosphatidylcholine were not found in the de novo transcriptome profile, though a choline phosphate transferase was found, suggesting that the Kennedy pathway is the principal route for the synthesis of PC. The fatty acid profiles of the four most abundant lipids suggested that these lipids were not generally converted between one another. This study shows for the first time that there are considerable changes in the biosynthesis of the three most abundant phospholipid classes in the normal cell cycle of D. quadricauda , by margins large enough to elicit changes to the physical properties of membranes.

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Structural studies of the lamellar to bicontinuous gyroid cubic (QGII) phase transitions under limited hydration conditions

Cited by 11 publications

References 38 publications

Structural Transitions in Ceramide Cubic Phases during Formation of the Human Skin Barrier

Structural Transitions in Ceramide Cubic Phases during Formation of the Human Skin Barrier

Crystal twinning of bicontinuous cubic structures

The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote

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