Structural studies on the hexose region of the lipopolysaccharide from Escherichia coli C

Jansson, Per‐Erik; Lindberg, Bengt; Bruse, Gert W.; Lindberg, Alf A.; Wollin, Ralph

doi:10.1016/s0008-6215(00)84816-8

Cited by 10 publications

(2 citation statements)

References 13 publications

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“…The attachment site for this glucose, therefore, could not be determined exactly. In Escherichia coli C, belonging also to the coli R1 type of core, a structure was found as shown in Formula 1 [22,23]. Because the phase I1 lipopolysaccharide also represents a serological coli R1 core [4], we assume that Sk.…”

Section: Discussionmentioning

confidence: 99%

Structure of the Hexose Region of Shigella sonnei Phase II Lipopolysaccharide with 3-Deoxy-d-manno-octulosonic Acid as Possible Immunodeterminant and Its Relation to Escherichia coli R1 Core

Kontrohr

Kocsis

1978

Eur J Biochem

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Section: Discussionmentioning

confidence: 99%

Structure of the Hexose Region of Shigella sonnei Phase II Lipopolysaccharide with 3-Deoxy-d-manno-octulosonic Acid as Possible Immunodeterminant and Its Relation to Escherichia coli R1 Core

Kontrohr

Kocsis

1978

Eur J Biochem

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“…Hep stands for L-glycero-D-manno-heptose (11) H HO+CH,OH HomH Ahhrevia/ions. Gal, galactose; Glc, glucose; GlcNAc, 2-acetamido-2-deoxyglucose; Hep, L-g/Scrro-D-manno-heptose; dOclA, 3-deoxy-~-manno-octulosonic acid.…”

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confidence: 99%

Structural Studies on the Hexose Region of the Core in Lipopolysaccharides from Enterobacteriaceae

Jansson

Lindberg

Lindberg³

et al. 2005

European Journal of Biochemistry

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245

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The, structures for the hexose regions of cores from Enterobacteriaceae lipopolysaccharides have been investigated, using specific degradations and 'H N M R studies as the principal methods. Complete structures for these regions in the Salmonella, the Escherichia coli R I , R2, R3, R4, the E. coli K12 and E. coli B cores are proposed. Some complementary information on the structure of the heptose region has also been obtained.

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Interaction between phage G13 and its oligosaccharide receptor studied by equilibrium dialysis

Bruse¹,

Wollin²,

Lindberg

1989

J of Molecular Recognition

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The reversible binding of phage G13, a phi X174-like single-strand DNA phage, to a 3H-labelled nonasaccharide from the lipopolysaccharide of its natural host Escherichia coli C was studied with equilibrium dialysis. The binding constant (Ka) was determined to 1.3 x 10(7) M-1 in Scatchard and Lineweaver-Burk plots. Approximately one saccharide bound per G13 phage particle which suggests that only one of the 12 spikes in each G13 virion was engaged in the phage/receptor saccharide interaction. Equilibrium dialysis inhibition experiments with saccharides from lipopolysaccharides of an isogenic series of Salmonella typhimurium mutants showed that hepta- and pentasaccharides from two G13-sensitive bacteria, i.e., with efficiencies of plating of 0.1-1.0 compared to E. coli C, were efficient inhibitors with Ka-values greater than or equal to 1.2 x 10(7) M-1. The octa- and hexasaccharides from two G13 resistant strains, with efficiency of plating less than or equal to x 10(-4), were either greater than 1000-fold or greater than 15-fold less efficient as inhibitors with Ka-values less than or equal to 8.8 x 10(5) M-1. The results show that phage G13 binds in a specific and reversible way to penta-, hepta-, and nonasaccharides from G13 sensitive bacteria with the specificity residing in the hexose and heptose region of the core lipopolysaccharide.

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Structural studies on the hexose region of the lipopolysaccharide from Escherichia coli C

Cited by 10 publications

References 13 publications

Structure of the Hexose Region of Shigella sonnei Phase II Lipopolysaccharide with 3-Deoxy-d-manno-octulosonic Acid as Possible Immunodeterminant and Its Relation to Escherichia coli R1 Core

Structure of the Hexose Region of Shigella sonnei Phase II Lipopolysaccharide with 3-Deoxy-d-manno-octulosonic Acid as Possible Immunodeterminant and Its Relation to Escherichia coli R1 Core

Structural Studies on the Hexose Region of the Core in Lipopolysaccharides from Enterobacteriaceae

Interaction between phage G13 and its oligosaccharide receptor studied by equilibrium dialysis

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