Structural variants of glucocorticoid receptor binding sites and different versions of positive glucocorticoid responsive elements: Analysis of GR-TRRD database

Меркулов, В. М.; Меркулова, Т. И.

doi:10.1016/j.jsbmb.2009.02.003

Cited by 29 publications

(22 citation statements)

References 83 publications

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“…Recently, studies have found that only half of GR binding sequences correspond to the GRE consensus sequence as inverted repeat of the exanucleotide TGTTCT. Indeed, 40% of GR binding sequences contain only the hexanucleotide and the majority of these genes are glucocorticoid-inducible genes (48). The active GRE within the regulatory regions of the Vdr gene we describe here contains the precise consensus sequence TGTTCT.…”

Section: Discussionmentioning

confidence: 91%

Dexamethasone Enhances 1α,25-Dihydroxyvitamin D3 Effects by Increasing Vitamin D Receptor Transcription

Hidalgo

Deeb

Pike

et al. 2011

Journal of Biological Chemistry

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Background: Dexamethasone increases vitamin D receptor and anti-proliferative effects of calcitriol. Result: Glucocorticoid receptor binds to response elements within regulatory region of Vdr gene to drive its transcription. Conclusion: Dexamethasone induces de novo transcription of the vitamin D receptor in a glucocorticoid receptor-dependent manner. Significance: A proposed mechanism by which glucocorticoids increase vitamin D receptor and calcitriol activities.

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Section: Discussionmentioning

confidence: 91%

Dexamethasone Enhances 1α,25-Dihydroxyvitamin D3 Effects by Increasing Vitamin D Receptor Transcription

Hidalgo

Deeb

Pike

et al. 2011

Journal of Biological Chemistry

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“…In order to explain a direct genomic action of testosterone, an attempt was made to identify ARE on the promoter of the mouse OAT gene. We carried out an in silico search for homology to the consensus ARE motifs by selecting either the left half-site 5'-AGAACA-3' or the right half-site 5'-TGTTCT-3' motifs by search computational using MatInspector (http://www.genomatix.de/) (Beato et al, 1989;Fabre et al, 1994;Roche et al, 1992;Wang et al, 2007;Merkulov & Merkulova, 2009). Several ARE (5'-AGAACAnnnTGTTCT-3') and glucocorticoid responsive element (GRE, 5'-GGTACAnnnTGTTCT-3') which share the same sequence 5'-TGTTCT-3' (Nelson et al, 1999, Verrijdt et al, 2003 were identified on the promoter of the mouse OAT gene.…”

Section: Discussionmentioning

confidence: 99%

Orchidectomy Upregulates While Testosterone Treatment Downregulates the Expression of Ornithine Aminotransferase Gene in the Mouse Kidney

Levillain¹,

Dégletagne²,

Letexier³

et al. 2011

Basic and Clinical Endocrinology Up-to-Date

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“…Not every site where GR specifically binds to the DNA represents a GRE. The binding sites for transcription factors on the DNA are regions whose affinity for the given protein are elevated because of specific nucleotide sequences that distinguish it from other regions (50). The GRbs found by Allgen Promo program within the IL-6-responsive element B (2146/288) of the Hp gene HRE have a typical hexanucleotide motif of a new variant of the palindromic nucleotide sequence G/AGnACAnnnTGTTCT.…”

Section: Discussionmentioning

confidence: 99%

“…Thereby, the binding of the GR monomer to the hexanucleotide motif is stabilized by another protein that possesses a binding site adjacent to the hexanucleotide (50). GRbs found within element C of the Hp gene HRE contain a hexanucleotide sequence (CAAACA) that slightly differs from the sequence found in element B (GGAACA).…”

Section: Discussionmentioning

confidence: 99%

Association of the glucocorticoid receptor with STAT3, C/EBPβ, and the hormone‐responsive element within the rat haptoglobin gene promoter during the acute phase response

et al. 2010

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SummaryUpregulation of haptoglobin (Hp) expression in the rat during the acute phase (AP) response is the result of synergistic effects of IL-6-, IL-1b-, and corticosterone-activated signaling pathways. IL-6 signaling terminates in cis-trans interactions of the Hp gene hormone-responsive element (HRE) with transcription factors STAT3 and C/EBPb. The aim of this study was to examine the unresolved molecular mechanism of glucocorticoid action. A 3-fold rise in serum corticosterone at 2 and 4 h of the AP response induced by turpentine administration preceded a 2.3-fold increase in the rate of Hp gene transcription at 12 h that was accompanied by a 4.8-fold increase in glucocorticoid receptor (GR), the appearance of an 86-kDa STAT3 isoform and 3.9-, 1.9-, and 1.7-fold increased amounts of 91-kDa STAT3, 35-and 42-kDa C/EBPb isoforms in the nucleus. These events resulted in 4.6-and 2.5-fold increased Hp levels in the liver and serum at 24 h. HRE affinity chromatography and immunoblot analysis revealed that maximal occupancy of the HRE with GR, STAT3, and C/EBPb at 12 h correlated with increased transcriptional activity of the Hp gene. Coimmunoprecipitation experiments showed that activated GR established de novo interaction with STAT3 isoforms while GR-C/EBPb interactions observed during basal transcription increased during the AP response. Computer analysis of the HRE disclosed two potential GR-binding sites: one overlapping STAT3, another adjacent to a C/EBPb-binding site. This finding and the experimental results suggest that activated GR through direct interactions with STAT3 and C/EBPb, participates in Hp gene upregulation as a transcriptional coactivator.

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Structural variants of glucocorticoid receptor binding sites and different versions of positive glucocorticoid responsive elements: Analysis of GR-TRRD database

Cited by 29 publications

References 83 publications

Dexamethasone Enhances 1α,25-Dihydroxyvitamin D3 Effects by Increasing Vitamin D Receptor Transcription

Dexamethasone Enhances 1α,25-Dihydroxyvitamin D3 Effects by Increasing Vitamin D Receptor Transcription

Orchidectomy Upregulates While Testosterone Treatment Downregulates the Expression of Ornithine Aminotransferase Gene in the Mouse Kidney

Association of the glucocorticoid receptor with STAT3, C/EBPβ, and the hormone‐responsive element within the rat haptoglobin gene promoter during the acute phase response

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