Structure–activity relationships of Erwinia carotovora quorum sensing signaling molecules

Welch, Martin; Dutton, Jenny M.; Glansdorp, Freija G.; Thomas, Gemma L.; Smith, Debra; Coulthurst, Sarah J.; Barnard, Anne M. L.; Salmond, George P. C.; Spring, David R.

doi:10.1016/j.bmcl.2005.06.066

Cited by 33 publications

(20 citation statements)

References 16 publications

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“…Volume ratio refers to the ratio of the normalized volumes of a pair of spots (the same spot of uncoiled and coiled forms), for example, a value of 2.0 represents a two-fold increase while -2.0 represents a two-fold decrease LuxR protein is a quorum sensor that functions as an interand intracellular communication species in both Gram positive and Gram negative bacteria [15]. In Gram negative bacteria, it also accepts the signal from the N-acyl-L-homoserine lactone (AHL) signal molecule [16]. This protein regulates cell motility and the activity of NADPH-dependent glucose-6-phosphate dehydrogenase, which is essential for glucose and acetate metabolism [15].…”

Section: Discussionmentioning

confidence: 99%

Revealing differentially expressed proteins in two morphological forms of Spirulina platensis by proteomic analysis

Hongsthong

Sirijuntarut²,

Prommeenate

et al. 2007

Mol Biotechnol

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Spirulina is distinguished from other cyanobacteria by its spiral morphology; however, this cyanobacterium has frequently been observed with a linear morphology in laboratory and industrial conditions. In our laboratory conditions, the simultaneously presence of the linear and spiral forms has also been observed. In the present study, the two forms of S. platensis C1 were separated and grown as axenic cultures in order to study the proteins that were differentially expressed in the soluble and insoluble protein fractions of the spiral and the linear forms. Two dimensional-differential gel electrophoresis (2D-DIGE) was performed to separate differentially expressed proteins that were subsequently identified by mass spectrometry. The differentially expressed proteins suggested two points. First, the morphological change is possibly induced by various environmental stresses such as oxygen level, carbon dioxide level, nutrient availability, and light. Second, the change of cell-shape might be a result of the change in a cell shape determination mechanism. Thus, this study is the first to show evidence at the protein level that may explain this morphological transformation in Spirulina.

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Section: Discussionmentioning

confidence: 99%

Revealing differentially expressed proteins in two morphological forms of Spirulina platensis by proteomic analysis

Hongsthong

Sirijuntarut²,

Prommeenate

et al. 2007

Mol Biotechnol

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“…More recently, Chatterjee et al [55] defined two classes of QS systems, using mainly 3-oxo-C8-HSL (Class I) or 3-oxo-C6-HSL (Class II) as autoinducer. This duality is linked to the structural characteristics of the NAHSL synthase (LuxI-type protein) encountered in each Pectobacterium strain [56], [57]. It is also related to the existence in Pectobacterium of two types of NAHSL receptors (LuxR-type proteins) that have a different affinity for each of the two NAHSLs [58]–[60].…”

Section: Discussionmentioning

confidence: 99%

“…Our results show that NAHSLs production is widespread within both old and emerging soft-rot bacteria isolates. Minor species of NAHSLs, likely to be less specific products of LuxI synthase or catabolites resulting from NAHSLs turnover, coexist with 3-oxo-C6-HSL and 3-oxo-C8-HSL, the two molecules identified as true QS signals [13], [56], [57]. However, the nature of these signals seems to be dependent on the bacterial genus or species.…”

Section: Discussionmentioning

confidence: 99%

Quorum Sensing Signaling Molecules Produced by Reference and Emerging Soft-Rot Bacteria (Dickeya and Pectobacterium spp.)

et al. 2012

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BackgroundSeveral small diffusible molecules are involved in bacterial quorum sensing and virulence. The production of autoinducers-1 and -2, quinolone, indole and γ-amino butyrate signaling molecules was investigated in a set of soft-rot bacteria belonging to six Dickeya or Pectobacterium species including recent or emerging potato isolates.Methodology/Principal FindingsUsing bacterial biosensors, immunoassay, and chromatographic analysis, we showed that soft-rot bacteria have the common ability to produce transiently during their exponential phase of growth the N-3-oxo-hexanoyl- or the N-3-oxo-octanoyl-l-homoserine lactones and a molecule of the autoinducer-2 family. Dickeya spp. produced in addition the indole-3-acetic acid in tryptophan-rich conditions. All these signaling molecules have been identified for the first time in the novel Dickeya solani species. In contrast, quinolone and γ-amino butyrate signals were not identified and the corresponding synthases are not present in the available genomes of soft-rot bacteria. To determine if the variations of signal production according to growth phase could result from expression modifications of the corresponding synthase gene, the respective mRNA levels were estimated by reverse transcriptase-PCR. While the N-acyl-homoserine lactone production is systematically correlated to the synthase expression, that of the autoinducer-2 follows the expression of an enzyme upstream in the activated methyl cycle and providing its precursor, rather than the expression of its own synthase.Conclusions/SignificanceDespite sharing the S-adenosylmethionine precursor, no strong link was detected between the production kinetics or metabolic pathways of autoinducers-1 and -2. In contrast, the signaling pathway of autoinducer-2 seems to be switched off by the indole-3-acetic acid pathway under tryptophan control. It therefore appears that the two genera of soft-rot bacteria have similarities but also differences in the mechanisms of communication via the diffusible molecules. Our results designate autoinducer-1 lactones as the main targets for a global biocontrol of soft-rot bacteria communications, including those of emerging isolates.

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“…3-Oxo-C6-HSL production has been demonstrated in a variety of Erwinia strains, including strains that do not make the carbapenem antibiotic (table 1). In addition, it has been shown (both in vitro and in vivo) that CarR Ecc is capable of responding to a variety of AHL signals, not just 3-oxo-C6-HSL, further widening the scope for cross-activation of Car production in Ecc by exogenously provided AHLs (Welch et al 2000(Welch et al , 2005. The presence of other bacteria may also help to increase the local 3-oxo-C6-HSL concentration (in the absence of further AHL synthesis), which could serve to activate Car production by Ecc.…”

Section: Why Quorum Sense?mentioning

confidence: 99%

Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria

Barnard

Bowden

Burr

et al. 2007

Phil. Trans. R. Soc. B

145

123

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Quorum sensing describes the ability of bacteria to sense their population density and respond by modulating gene expression. In the plant soft-rotting bacteria, such as Erwinia, an arsenal of plant cell wall-degrading enzymes is produced in a cell density-dependent manner, which causes maceration of plant tissue. However, quorum sensing is central not only to controlling the production of such destructive enzymes, but also to the control of a number of other virulence determinants and secondary metabolites. Erwinia synthesizes both N-acylhomoserine lactone (AHL) and autoinducer-2 types of quorum sensing signal, which both play a role in regulating gene expression in the phytopathogen. We review the models for AHL-based regulation of carbapenem antibiotic production in Erwinia. We also discuss the importance of quorum sensing in the production and secretion of virulence determinants by Erwinia, and its interplay with other regulatory systems.

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Structure–activity relationships of Erwinia carotovora quorum sensing signaling molecules

Cited by 33 publications

References 16 publications

Revealing differentially expressed proteins in two morphological forms of Spirulina platensis by proteomic analysis

Revealing differentially expressed proteins in two morphological forms of Spirulina platensis by proteomic analysis

Quorum Sensing Signaling Molecules Produced by Reference and Emerging Soft-Rot Bacteria (Dickeya and Pectobacterium spp.)

Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria

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