Structure-Activity Relationships of Saponins from<i>Gleditsia sinensis</i>in Cytotoxicity and Induction of Apoptosis<i />

Zhong, Lei; Li, Ping; Han, Jian; Qu, Guiqin; Guo, Dean

doi:10.1055/s-2004-827225

Cited by 27 publications
(17 citation statements)

References 11 publications

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“…According to the literature, the effects of saponins on the digestive system are diverse because they can act both as protectors against ulcerative lesions and as destructors of the cellular membrane. Saponins have also been described as enhancers of gastric permeability and natural enhancers of tumor cell apoptosis (Chow et al, 2002;Zhong et al, 2004), supporting the notion that the biological effects of saponins vary among species.…”

Section: Histology Of the Crop Regionmentioning
confidence: 66%

Molluscicidal effects of saponin-rich plant extracts on the grey field slug
González-Cruz
¹
,
Martín
²

2013
Cienc. Inv. Agr.
17
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10
0
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D. González-Cruz, and R. San Martín. 2013. Molluscicidal effects of saponin-rich plant extracts on the grey field slug. Cien. Inv. Agr. 40(2): 341-349. Slug control is a major concern in crop fields because these mollusks consume and damage plants that are destined for human consumption and ornamentation. The grey field slug Deroceras reticulatum (Müller, 1774) is one of the most commonly occurring mollusks with agronomic importance, consuming up to 80% of the leaf surface of raps and other crops. The major difficulty in slug control is that the main products used for this purpose can also endanger non-target animals, such as pets and birds. New methods of control that are naturally based and environmental friendly are urgently required, both for the farmer who applies the product and for the final consumer. In this study, we analyzed the anti-molluscal activity of extracts from three plant with a high content of saponins: Camellia oleifera, Gleditsia amorphoides and Quillaja saponaria. Forced oral injection and histological analyses of the digestive system were performed to measure the effects of the plant extracts against slugs. All three extracts showed anti-feeding activity and toxicity against slugs when injected orally at a range of concentrations between 1 and 4% w/w. All three extracts also damaged the gastric epithelium of the crop region of the slugs, suggesting that they show strong membranolytic properties in the digestive system of these mollusks. This study revealed novel natural compounds that can potentially be used in the control of grey field slugs. The data hereby presented could be useful in future comparative studies between other mollusks with agronomic importance.

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Section: Histology Of the Crop Regionmentioning
confidence: 66%

Molluscicidal effects of saponin-rich plant extracts on the grey field slug
González-Cruz
¹
,
Martín
²

2013
Cienc. Inv. Agr.
17
1
10
0
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“…Chinese herbal antitumor agents, including assolanine (6), an extract from Rhodiola rosea rhizomes (7), Gleditsioside E from Gleditsia sinensis (8) and saponins from Gleditsia sinensis (9), exert significant effects against a variety of tumors and have been confirmed to be effective antitumor agents. Saffron is a derived plant product from the dried stigma of the Crocus sativus flower (family, Iridaceae), which has been used for several hundred years in the treatment of cardiovascular diseases, inflammatory diseases and several other tumors (10,11).…”

Section: Introductionmentioning
confidence: 99%

Crocetin induces apoptosis of BGC-823 human gastric cancer cells
He
¹
,
Si
²
,
Wang
³

et al. 2013
Molecular Medicine Reports
29
1
17
0
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Abstract. Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin-sensitive treatment of GC and its possible mechanisms. BGC-823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC-823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase-3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose-and time-dependent manner. Apoptotic BGC-823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC-823 cells. Crocetin also induced caspase-3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC-823 cells, and may be used as an effective agent in the treatment of GC.

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“…Twelve pure saponins were isolated from G. sinensis, and were fully characterized as reported in our previous paper [9]. They include gleditsia saponins B, C, and C′ (abbreviated as GSB, GSC, and GSC'), and gleditsiosides A-D, F, G, N, O, and Q (abbreviated as GA, GB, GC, GD, GF, GG, GN, GO, and GQ).…”

Section: Chemicals and Reagentsmentioning
confidence: 99%

“…Moreover, they could be acylated by one or two monoterpenic acid groups at the α-chain. The monoterpenic acid residue, either 6(R)-6-hydroxy-2,6-dimethyl 2,7-octadienolic acid (menthiafolic acid) or 6(R)-6-hydroxy-2-hydroxymethyl-6-methyl 2,7-octadienolic acid, is critical for the cytotoxic activities of these saponins [9]. Although Gleditsia saponins appear to be a promising antitumor agent, the composition has not been well addressed thus far.…”

mentioning
confidence: 99%

Separation and Characterization of Triterpenoid Saponins in Gleditsia sinensis by Comprehensive Two-Dimensional Liquid Chromatography Coupled with Mass Spectrometry
Wang
¹
,
Wang
²
,
Qiao
³

et al. 2016
Planta Med
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8
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A comprehensive two-dimensional liquid chromatography coupled with mass spectrometry method was established to separate and characterize triterpenoid saponins in , the Chinese herbal medicine Zhu-Ya-Zao used for the treatment of apoplexy. The saponins were separated on an Agilent Zorbax Eclipse plus C column (2.1 mm × 150 mm, 1.8 µm) for the first dimension, and a Poroshell 120 phenyl-hexyl column (3.0 mm × 50 mm, 2.7 µm) for the second dimension. Methanol and acetonitrile were used as the organic mobile phase for D andD, respectively. The theoretical peak capacity was 640, and the orthogonality was 57 %. Particularly, saponins with different numbers of monoterpene groups could be well separated on the second dimension. The structures were characterized by electrospray ionization tandem mass spectrometry in both positive and negative ion modes. In the MS/MS spectra, the relative abundances for [B + Na] and [Y + Na] were closely correlated with the number of sugar residues of the -chain and-chain, respectively, which facilitated the differentiation of isomers. Finally, a total of 72 saponins with molecular weights greater than 1500 Da were characterized. Among them, 49 compounds, including 2 acetylated saponins, were detected from for the first time.

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Structure-Activity Relationships of Saponins fromGleditsia sinensisin Cytotoxicity and Induction of Apoptosis

Cited by 27 publications

References 11 publications

Molluscicidal effects of saponin-rich plant extracts on the grey field slug

Molluscicidal effects of saponin-rich plant extracts on the grey field slug

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Separation and Characterization of Triterpenoid Saponins in Gleditsia sinensis by Comprehensive Two-Dimensional Liquid Chromatography Coupled with Mass Spectrometry

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