Structure analysis of thymidylate synthase from white spot syndrome virus reveals WSSV-specific structural elements

Panchal, Vipul; Kumar, Sushil; Hossain, Shaikh Nausad; Vasudevan, Dileep

doi:10.1016/j.ijbiomac.2020.11.071

Cited by 4 publications

(13 citation statements)

References 35 publications

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“…In the past 20 years, shrimp diseases have caused critical economic losses that seriously threaten farming practices, of which white spot syndrome (WSS) is the deadliest viral disease caused by white spot syndrome virus (WSSV) [ 15 ]. WSSV causes up to 80–100% mortality of infected shrimp within 5–10 days, thus leading to great economic loss [ 16 ]; the total economic loss from this disease is estimated to be approximately 8–15 billion USD, and this continues to increase by 1 billion USD (10% of global shrimp production) annually [ 17 , 18 ].…”

Section: Dna Viral Diseasesmentioning

confidence: 99%

Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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Shrimp is one of the most valuable aquaculture species globally, and the most internationally traded seafood product. Consequently, shrimp aquaculture practices have received increasing attention due to their high value and levels of demand, and this has contributed to economic growth in many developing countries. The global production of shrimp reached approximately 6.5 million t in 2019 and the shrimp aquaculture industry has consequently become a large-scale operation. However, the expansion of shrimp aquaculture has also been accompanied by various disease outbreaks, leading to large losses in shrimp production. Among the diseases, there are various viral diseases which can cause serious damage when compared to bacterial and fungi-based illness. In addition, new viral diseases occur rapidly, and existing diseases can evolve into new types. To address this, the review presented here will provide information on the DNA and RNA of shrimp viral diseases that have been designated by the World Organization for Animal Health and identify the latest shrimp disease trends.

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Section: Dna Viral Diseasesmentioning

confidence: 99%

Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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“…Primary cell cultures of shrimp lymphoid organs infected with WSSV had shown a high rate of viral replication compared with host replication, implying that the incorporation of nucleotide precursors has an important role 31 . In this context, TS that catalyses the synthesis of DNA precursors, dTMP from dUMP, becomes relevant.…”

Section: Crystal Structure Of Tsmentioning

confidence: 99%

“…wTS adopted a helical conformation in contrast to a loop conformation in PvTS (Figure 4d). This may be attributed to a unique Ser280 in wTS in place of a proline residue in other TS structures 31 . In addition, in contrast to PvTS that allows only restricted access of ligand to its binding pocket, wTS remains fairly accessible to ligand binding.…”

Section: Crystal Structure Of Tsmentioning

confidence: 99%

“…This is owing to the positioning of its catalytic residues Cys170 and the RTG loop comprising of residues 22–28 in its binding pocket, away from the C‐terminal end (Figure 4e). This difference in the spatial arrangement of the C‐terminal region in wTS that makes the ligand‐binding pocket accessible has been suggested to have the potential to be exploited for targeting using specific inhibitors 31 . Separately, the crystal structure of dUMP and methotrexate bound wTS has also been solved, revealing each monomer being bound to a dUMP, with methotrexate lying close to it.…”

Section: Crystal Structure Of Tsmentioning

confidence: 99%

“…In addition, the enzyme thymidylate synthase (TS), wTS from WSSV that catalyses the synthesis of 5′‐deoxythymidine monophosphate (dTMP), the precursor essential for DNA replication, from deoxyuridine monophosphate (dUMP), has also been structurally characterised (Table 1). 31 …”

Section: Introductionmentioning

confidence: 99%

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Structural insights into proteins from white spot syndrome virus: Perspectives on structure‐based anti‐viral strategies

Datta

Hossain

Mohapatra

et al. 2022

Reviews in Aquaculture

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White spot syndrome virus (WSSV) causes severe disease in crustaceans, specifically shrimp, leading to substantial economic losses to the aquaculture industry. Existing treatment methods fail to sufficiently curb the occurrence and spread of the disease, necessitating the development of effective control and treatment strategies. Structural studies have primarily focused on envelope proteins and a few non‐structural proteins involved in host entry, viral replication, viral assembly and host immune evasion, thus presenting themselves as potential drug targets. Here, we provide a comprehensive review of the insights gained from such structural elucidations to contribute to developing novel therapeutic strategies. In addition, we touch upon the current and future perspectives towards structure‐based drug or vaccine development against WSSV.

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SpSR-B2 functions as a potential pattern recognition receptor involved in antiviral and antibacterial immune responses of mud crab Scylla paramamosain

Zhou

Wang

et al. 2021

International Journal of Biological Macromolecules

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Structure analysis of thymidylate synthase from white spot syndrome virus reveals WSSV-specific structural elements

Cited by 4 publications

References 35 publications

Viral Shrimp Diseases Listed by the OIE: A Review

Viral Shrimp Diseases Listed by the OIE: A Review

Structural insights into proteins from white spot syndrome virus: Perspectives on structure‐based anti‐viral strategies

SpSR-B2 functions as a potential pattern recognition receptor involved in antiviral and antibacterial immune responses of mud crab Scylla paramamosain

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