Structure and Biocompatibility of an Injectable Bone Regeneration Composite

Tan, Rongwei; Feng, Qingling; He, Jinguang; Li, Jinyu; Yu, Xing; She, Zhigang; Wang, Ming-Bo; Liu, Huanye

doi:10.1163/092050610x528561

Cited by 11 publications

(10 citation statements)

References 62 publications

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“…Such a structure can store water, proteins, or drugs in its pores. However, the pore size of the 3D network decreased as the calcium alginate concentration was increased [33]. Larger pores can store more water or drugs, but also result in decreased stability.…”

Section: Discussionmentioning

confidence: 99%

Drug-Loadable Calcium Alginate Hydrogel System for Use in Oral Bone Tissue Repair

Chen

Shen

Komasa

et al. 2017

IJMS

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This study developed a drug-loadable hydrogel system with high plasticity and favorable biological properties to enhance oral bone tissue regeneration. Hydrogels of different calcium alginate concentrations were prepared. Their swelling ratio, degradation time, and bovine serum albumin (BSA) release rate were measured. Human periodontal ligament cells (hPDLCs) and bone marrow stromal cells (BMSCs) were cultured with both calcium alginate hydrogels and polylactic acid (PLA), and then we examined the proliferation of cells. Inflammatory-related factor gene expressions of hPDLCs and osteogenesis-related gene expressions of BMSCs were observed. Materials were implanted into the subcutaneous tissue of rabbits to determine the biosecurity properties of the materials. The materials were also implanted in mandibular bone defects and then scanned using micro-CT. The calcium alginate hydrogels caused less inflammation than the PLA. The number of mineralized nodules and the expression of osteoblast-related genes were significantly higher in the hydrogel group compared with the control group. When the materials were implanted in subcutaneous tissue, materials showed favorable biocompatibility. The calcium alginate hydrogels had superior osteoinductive bone ability to the PLA. The drug-loadable calcium alginate hydrogel system is a potential bone defect reparation material for clinical dental application.

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Section: Discussionmentioning

confidence: 99%

Drug-Loadable Calcium Alginate Hydrogel System for Use in Oral Bone Tissue Repair

Chen

Shen

Komasa

et al. 2017

IJMS

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“…[12, 13, 22, 23] Hydrogels implanted in rat cranial defects in previous studies were often surrounded by fibrous tissue, and required use of growth factors, encapsulated cells, or mineral composites to facilitate infiltration of bone tissue. [23–27] Contrastingly, the majority of hydrogels used in the study described in this manuscript, including the subset of acellular hydrogels, demonstrated direct bone-to-implant contact with bone infiltration into the hydrogel, rather than the fibrous tissue response commonly associated with biomaterials implanted into the body. [28] Bone tissue infiltrating into the hydrogels indicates these hydrogels have the potential to integrate with host tissue and accelerate bone formation, and may be a consequence of improved cellular interactions secondary to the presence of phosphate groups in both of the hydrogel formulations used.…”

Section: 4 Discussionmentioning

confidence: 99%

Biodegradable, phosphate-containing, dual-gelling macromers for cellular delivery in bone tissue engineering

Watson¹,

Vo²,

Tatara³

et al. 2015

Biomaterials

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Injectable, biodegradable, dual-gelling macromer solutions were used to encapsulate mesenchymal stem cells (MSCs) within stable hydrogels when elevated to physiologic temperature. Pendant phosphate groups were incorporated in the N-isopropyl acrylamide-based macromers to improve biointegration and facilitate hydrogel degradation. The MSCs were shown to survive the encapsulation process, and live cells were detected within the hydrogels for up to 28 days in vitro. Cell-laden hydrogels were shown to undergo significant mineralization in osteogenic medium. Cell-laden and acellular hydrogels were implanted into a critical-size rat cranial defect for 4 and 12 weeks. Both cell-laden and acellular hydrogels were shown to degrade in vivo and help to facilitate bone growth into the defect. Improved bone bridging of the defect was seen with the incorporation of cells, as well as with higher phosphate content of the macromer. Furthermore, direct bone-to-hydrogel contact was observed in the majority of implants, which is not commonly seen in this model. The ability of these macromers to deliver stem cells while forming in situ and subsequently degrade while facilitating bone ingrowth into the defect makes this class of macromers a promising material for craniofacial bone tissue engineering.

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“…38 In addition, when a scaffold containing alginate is placed in the medium, there is uncontrolled degradation of ionically cross-linked alginate due to the loss of divalent cations. 39,40 This leads to the formation of pores inside the scaffold, which enhances cell migration. 41 Although scaffolds provide templates for bone regeneration, biologic factors such as cells, growth factors, or genes are typically required to effectively repair challenging bone defects.…”

Section: Introductionmentioning

confidence: 99%

Integration of a Novel Injectable Nano Calcium Sulfate/Alginate Scaffold andBMP2Gene-Modified Mesenchymal Stem Cells for Bone Regeneration

Dziak

Mao

et al. 2013

Tissue Engineering Part A

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The repair of craniofacial bone defects is surgically challenging due to the complex anatomical structure of the craniofacial skeleton. Current strategies for bone tissue engineering using a preformed scaffold have not resulted in the expected clinical regeneration due to difficulty in seeding cells into the deep internal space of scaffold, and the inability to inject them in minimally invasive surgeries. In this study, we used the osteoconductive and mechanical properties of nano-scale calcium sulfate (nCS) and the biocompatibility of alginate to develop the injectable nCS/alginate (nCS/A) paste, and characterized the effect of this nCS/A paste loaded with bone morphogenetic protein 2 (BMP2) gene-modified rat mesenchymal stem cells (MSCs) on bone and blood vessel growth. Our results showed that the nCS/A paste was injectable under small injection forces. The mechanical properties of the nCS/A paste were increased with an increased proportion of alginate. MSCs maintained their viability after the injection, and MSCs and BMP2 gene-modified MSCs in the injectable pastes remained viable, osteodifferentiated, and yielded high alkaline phosphatase activity. By testing the ability of this injectable paste and BMP2-gene-modified MSCs for the repair of critical-sized calvarial bone defects in a rat model, we found that BMP2-gene-modified MSCs in nCS/A (nCS/A+M/B2) showed robust osteogenic activity, which resulted in consistent bone bridging of the bone defects. The vessel density in nCS/A+M/B2 was significantly higher than that in the groups of blank control, nCS/A alone, and nCS/A mixed with MSCs (nCS/A+M). These results indicate that BMP2 promotes MSCs-mediated bone formation and vascularization in nCS/A paste. Overall, the results demonstrated that the combination of injectable nCS/A paste and BMP2-gene-modified MSCs is a new and effective strategy for the repair of bone defects.

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Structure and Biocompatibility of an Injectable Bone Regeneration Composite

Cited by 11 publications

References 62 publications

Drug-Loadable Calcium Alginate Hydrogel System for Use in Oral Bone Tissue Repair

Drug-Loadable Calcium Alginate Hydrogel System for Use in Oral Bone Tissue Repair

Biodegradable, phosphate-containing, dual-gelling macromers for cellular delivery in bone tissue engineering

Integration of a Novel Injectable Nano Calcium Sulfate/Alginate Scaffold andBMP2Gene-Modified Mesenchymal Stem Cells for Bone Regeneration

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