Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii

Choudhuri, Indranil; Khanra, Kalyani; Maity, Prasenjit; Patra, Anutosh; Maity, Gajendra Nath; Pati, Biswamoy; Nag, Anish; Mondal, Soumitra; Bhattacharyya, Nandan

doi:10.1016/j.ijbiomac.2020.12.063

Cited by 23 publications

(9 citation statements)

References 78 publications

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“…EPSwk presented an Mw of 6.31 × 10 5 Da, calculated from the standard curve. This result complies with the literature because for polysaccharide materials, including EPSs of microbial origin, the Mw values vary from 10 4 -10 6 Da [50]. According to Mnif and Ghribi [51], EPSs are high molecular weight polysaccharides.…”

Section: Determination Of Molecular Weight (Mw)supporting

confidence: 89%

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

et al. 2022

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The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

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Section: Determination Of Molecular Weight (Mw)supporting

confidence: 89%

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

et al. 2022

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“…In addition, the antioxidant capacities are also closely related to the molecular weight. Exopolysaccharides with a higher molecular weight have larger organic groups than polysaccharides with a lower molecular weight, which contribute to increased antioxidant activity [ 49 ]. However, other studies have found that low-molecular-weight EPS can also have high antioxidant capacity [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Genome Analysis of Bifidobacterium Bifidum E3, Structural Characteristics, and Antioxidant Properties of Exopolysaccharides

Yue

Wang

Han

et al. 2023

Foods

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In this study, the antioxidant properties of intact cells (IC), cell-free supernatant (CFS), and cell-free extracts (CFE) and whole genome sequencing of Bifidobacterium bifidum E3 (B. bifidum E3), as well as the structural characteristics and antioxidant properties of EPS-1, EPS-2, and EPS-3, were evaluated. The results revealed that intact cells (IC), cell-free supernatant (CFS), and cell-free extracts (CFE) had potent DPPH (1,1-Diphenyl-2-picrylhydrazyl radical), hydroxyl, and superoxide anion radical scavenging capacities, among which CFS was the best. At the genetic level, we identified a strong carbohydrate metabolism capacity, an EPS synthesis gene cluster, and five sugar nucleotides in B. bifidum E3. Therefore, we extracted cEPS from B. bifidum E3 and purified it to obtain EPS-1, EPS-2, and EPS-3. EPS-1, EPS-2, and EPS-3 were heteropolysaccharides with an average molecular weight of 4.15 × 104 Da, 3.67 × 104 Da, and 5.89 × 104 Da, respectively. The EPS-1 and EPS-2 are mainly comprised of mannose and glucose, and the EPS-3 is mainly comprised of rhamnose, mannose, and glucose. The typical characteristic absorption peaks of polysaccharides were shown in Fourier transform infrared spectroscopy (FT-IR spectroscopy). The microstructural study showed a rough surface structure for EPS-1, EPS-2, and EPS-3. Furthermore, EPS-1, EPS-2, and EPS-3 exhibited potent DPPH, hydroxyl, and superoxide anion radical scavenging capacities. Correlation analysis identified that antioxidant capacities may be influenced by various factors, especially molecular weight, chemical compositions, and monosaccharide compositions. In summary, the EPS that was produced by B. bifidum E3 may provide insights into health-promoting benefits in humans.

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“…In this study, the phosphomolbdenum assay was also studied. This approach is based on antioxidant agents reducing phosphate-molybdenum (VI) to phosphate-molybdenum (V) and forming a green complex at an acidic pH ( Choudhuri et al, 2021 ). The reduction of Mo (VI) increased with the increase of concentration of EPS-SL and EPS-N5, and the maximum reduction was 0.594 and 1.0105, respectively ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

et al. 2022

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Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii

Cited by 23 publications

References 78 publications

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

Biopolymer from Water Kefir as a Potential Clean-Label Ingredient for Health Applications: Evaluation of New Properties

Genome Analysis of Bifidobacterium Bifidum E3, Structural Characteristics, and Antioxidant Properties of Exopolysaccharides

Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

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