Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae

Wei, Chaoyang; Li, Weiqi; Shao, Shuang-shuang; He, Li; Cheng, Junwen; Han, Sufang; Liu, Yu

doi:10.1016/j.carbpol.2015.09.088

Cited by 68 publications

(26 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For NMR spectroscopic analysis, IPS fractions (60 mg) were dissolved in 0.8 mL D 2 O and lyophilized three times. Before transferring into NMR microtubes, samples were finally dissolved in 0.6 mL of high quality D 2 O containing 0.1 μL acetone [38]. The chemical shifts of the purified fractions showed positive signals.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharides from the Edible Mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam Show Anti-hypoxia Activities in Pulmonary Artery Smooth Muscle Cells

Jiao

Kuang

et al. 2019

IJMS

View full text Add to dashboard Cite

Three kinds of new water-soluble polysaccharides (FA, FB and FC) were isolated from wild mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam by the classical method “water extraction and alcohol precipitation” and purified by column chromatography. The Mw of FA, FB and FC ranged from 5690 Da to 38,340 Da. The three polysaccharide fractions in the fruiting body were mainly composed of 4 kinds of monosaccharides, including glucose, galactose, mannose, and arabinose, among which glucose and galactose were the major monosaccharides. The FTIR and NMR spectroscopy indicated that the skeleton of three fractions composed of a (1→4)-α-D-glycosidic backbone containing α-D-mannopyranose. In vitro anti-hypoxia activity data showed that three polysaccharide fractions possessed a significant effect on inhibiting PASM cells apoptosis under hypoxia. Among them, FC at the concentration of 200 µg/mL revealed a significant anti-hypoxia effect. These results revealed that the intracellular polysaccharides possessed potent anti-hypoxic activity, which might be related to inhibiting LDH and NADPH oxidase expression and promoting the formation of 5-hydroxytryptamine, dopamine, endothelins, acetylcholine. More importantly, FC showed good performance inducing KV1.5 expression and prohibiting KIR6.2 formation at protein level.

show abstract

Section: Methodsmentioning

confidence: 99%

Polysaccharides from the Edible Mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam Show Anti-hypoxia Activities in Pulmonary Artery Smooth Muscle Cells

Jiao

Kuang

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…In addition, Isaria cicadae conidia are commonly known as cicadas “seed”, the formation process is as follows: the larva of cicada is infected and parasitised by Paecilomyces before emergence; the fungus absorbs the polypide nutrient and turns into a mycelium; the mycelium transforms from a nutritive into a sexual stage; it then gradually branches and blooms from the top, forming the “ Isaria cicadae conidia” which is essential for reproductive functions (Wei et al 2016). …”

Section: Introductionmentioning

confidence: 99%

Isaria cicadae conidia possess antiproliferative and inducing apoptosis properties in gynaecological carcinoma cells

Sun

Wang

et al. 2017

Mycology

View full text Add to dashboard Cite

Isaria cicadae is an entomogenous fungus that has been used as a traditional Chinese medicinal materials to treat different diseases, including cancer. However, Isaria cicadae conidia for inhibitory activity against breast cancer cells growth are still not systematically studied. The present aim was to elucidate the phytochemical composition of Isaria cicadae conidia and to explore relevant anti-cancer potential in gynaecological carcinoma MCF-7 and Hela cells. Isaria cicadae conidia were identified by UPLC-ESI-Q-TOF-MS: high performance liquid chromatography-electrospray/quadrupole time of flight tandem mass spectrometry technology. Eight main compounds were identified which are nucleosides, cordycepic acid, cordycepin, beauvericin and myriocin by MS fragmentation ions. The nuclear morphology indicated the typical characteristics of apoptosis by Hoechst staining. Annexin V/PI staining revealed that the number of apoptotic cells was increased by Isaria cicadae conidia treatment. Furthermore, Isaria cicadae conidia also induced the caspase-mediated mitochondrial apoptosis pathway. The findings suggest that the full-scale active ingredients highlight the significance of Isaria cicadae conidia as potential anti-cancer agent in China.

show abstract

“…The injection volume was 300 μL for each test in triplicate. Data acquisition and further analysis were conducted with the ASTRA software (Version 6.0, Wyatt Technology, Goleta, CA, USA) [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology

Jiao²,

et al. 2017

Molecules

View full text Add to dashboard Cite

With the rising awareness of microbial exopolysaccharides (EPSs) application in various fields, halophilic microorganisms which produce EPSs have received broad attention. A newly identified Kocuria rosea ZJUQH CCTCC M2016754 was determined to be a moderate halobacterium on account of its successful adaption to the environment containing 10% NaCl. The optimal combination of fermentation medium compositions on EPS production was studied. In this work, a fractional factorial design was adopted to investigate the significant factors that affected EPS production. The factors of KCl and MgSO4 were found to have a profound impact on EPS production. We utilized central composite design and response surface methodology to derive a statistical model for optimizing the submerged culture medium composition. Judging from these experimental results, the optimum culture medium for producing EPSs was composed of 0.50% casein hydrolysate, 1.00% sodium citrate, 0.30% yeast extract, 0.50% KCl, 0.50% peptone, and 5.80% MgSO4 (initial pH 7.0). The maximal EPS was 48.01 g/L, which is close to the predicted value (50.39 g/L). In the validation experiment, the highest concentration of 70.64 g/L EPSs was obtained after 120 h under the optimized culture medium in a 5-L bioreactor. EPS from this bacterium was also characterized by differential scanning calorimetry (DSC) and Fourier transform infrared analysis (FT-IR). The findings in this study imply that Kocuria rosea ZJUQH has great potential to be exploited as a source of EPSs utilized in food, the pharmaceutical and agriculture industry, and in the biotreatment of hypersaline environments.

show abstract

Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae

Cited by 68 publications

References 57 publications

Polysaccharides from the Edible Mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam Show Anti-hypoxia Activities in Pulmonary Artery Smooth Muscle Cells

Polysaccharides from the Edible Mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam Show Anti-hypoxia Activities in Pulmonary Artery Smooth Muscle Cells

Isaria cicadae conidia possess antiproliferative and inducing apoptosis properties in gynaecological carcinoma cells

Optimization of EPS Production and Characterization by a Halophilic Bacterium, Kocuria rosea ZJUQH from Chaka Salt Lake with Response Surface Methodology

Contact Info

Product

Resources

About