Structure and conformational stability of a tetrameric thermostableN‐succinylamino acid racemase

Abstract

ABSTRACT:The N-succinylamino acid racemases (NSAAR) belong to the enolase superfamily and they are large homooctameric/hexameric species that require a divalent metal ion for activity. We describe the structure and stability of NSAAR from Geobacillus kaustophilus

Cysteine Mutational Studies Provide Insight into a Thiol-Based Redox Switch Mechanism of Metal and DNA Binding in FurA fromAnabaenasp. PCC 7120

“…Only in those cases where there were no distortions in the spectra, the resulting difference spectra were used for analysis. To determine the amount of secondary structure components, the Amide I band in D 2 O was decomposed into its constituents by curve fitting, using a line shape that was a combination of Gaussian and Lorentzian functions (43).…”

Rational re-design of the “double-racemase hydantoinase process” for optically pure production of natural and non-natural l-amino acids

“…Evaluation of "hydantoinase process" variations for l-amino acid production Recombinant enzymes l-N-carbamoylase from Geobacillus stearothermophilus CECT43 (BsLcar) and N-succinyl-amino acid racemase from G. kaustophilus CECT4264 (GkNSAAR) were obtained as previously described by our group with over 95% purity [36,40] (Fig. 2).…”

N-succinylamino acid racemases: Enzymatic properties and biotechnological applications

“…NSARs have been successfully purified from wild-type organisms [46,47] or overexpressed/purified from recombinant organisms [48][49][50][51][52][53][54][55][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75]. Members from the NSAR/OSBS subfamily have been described as dimers (Enterococcus, Listeria), tetramers (Geobacillus), hexamers (Streptomyces) or octamers 3 (Amycolatopsis, Deinococcus, Thermus, Sebekia) ( Table 2).…”

“…Several works at the beginning of the 21 st century focused on deciphering the natural function of NSARs and some evolutionary aspects [51,57,58,[69][70][71][72][73][74][75][76] (see below), allowing to localize new NSARs in Bacillus/Geobacillus genera. Some of the NSARs belonging to this genera have been highly characterized and/or applied to the production of optically pure amino acids [27][28][29][30][31][32][33][34][35][36][37][38]59,[61][62][63][64][65][66][67][68]. A different N-succinyl arginine/lysine racemase from Bacillus cereus ATCC 14579, highly specific for NS-Arg and NS-Lys has been reported, although it does not belong to the NSAR group reviewed in this paper [59].…”