Structure and Cytocompatibility of a Porous Biomimetic Material for Oral Tissue Wound Healing

Abstract

Composite materials containing natural biocompatible polymers, specific for the extracellular matrix present in the periodontal tissue, are prepared by mixing type I soluble collagen (COL) and fibronectin (FN) as fibrillar proteins and chondroitin sulfate (CS) as polysaccharidic component. The threedimensional porous structure is obtained by freeze-drying process and it is analyzed by scanning electron microscopy (SEM). Specific techniques of immunohistochemistry and transmission electron microscopy (TEM) are used to investigate the distribution and interaction between polymeric components within the scaffold. Light and electron microscopy observations are also performed on solutions of CS and FN to reveal their structural and morphological properties. The cytocompatibility of composite material is tested in gingival fibroblasts and osteoblast cell cultures using quantitative assays of cell viability. The results demonstrate that COL-CS-FN composite material has optimal characteristics for further testing as device for oral tissue wound healing.

Chondroitin sulfate-based composites: a tour d’horizon of their biomedical applications

“…The researcher has suggested further in vivo studies to confirm the biological activity of Coll-CS-FN composite material to check its interaction with the human dental pulp and support the promising results. 378…”

“…The researcher has suggested further in vivo studies to confirm the biological activity of Coll-CS-FN composite material to check its interaction with the human dental pulp and support the promising results. 378 Traditional marketed solutions focus primarily on bacterial growth suppression at the wound site, ignoring tissue repair, substantially impacting the healing pace. 379 It would be highly advantageous if a wound healing substance that is both antibacterial and tissue regenerating could be produced.…”

Anti-Inflammatory Activity of Biomaterials Intended for Periodontal Disease Treatment

“…Two types of biomaterials were prepared, one as a polymeric composite based on components of the extracellular matrix, collagen, chondroitin sulfate, and fibronectin [3] and one as a hybrid material by adding silver nanoparticles. Ultrastructural observations were performed by SEM with a Hitachi SU 1510 equipment (Tokyo, Japan), operated at 15 kV, in nitrogen atmosphere.…”