Clusters of galaxies are self-gravitating systems of mass ∼ 10 14 -10 15 h −1 M and size ∼ 1-3h −1 Mpc. Their mass budget consists of dark matter (∼ 80%, on average), hot diffuse intracluster plasma ( 20%) and a small fraction of stars, dust, and cold gas, mostly locked in galaxies. In most clusters, scaling relations between their properties, like mass, galaxy velocity dispersion, X-ray luminosity and temperature, testify that the cluster components are in approximate dynamical equilibrium within the cluster gravitational potential well. However, spatially inhomogeneous thermal and non-thermal emission of the intracluster medium (ICM), observed in some clusters in the X-ray and radio bands, and the kinematic and morphological segregation of galaxies are a signature of non-gravitational processes, ongoing cluster merging and interactions. Both the fraction of clusters with these features, and the correlation between the dynamical and morphological properties of irregular clusters and the surrounding large-scale structure increase with redshift.In the current bottom-up scenario for the formation of cosmic structure, where tiny fluctuations of the otherwise homogeneous primordial density field are amplified by gravity, clusters are the most massive nodes of the filamentary large-scale structure of the cosmic web and form by anisotropic and episodic accretion of mass, in agreement with most of the observational evidence. In this model of the universe dominated by cold dark matter, at the present time most baryons are expected to be in a diffuse component rather than in stars and A. Diaferio ( ) Dipartimento