Structure and evolution of a highly repetitive DNA sequence from Brassica napus

Xia, X. Y.; Selvaraj, Gopalan; Bertrand, Helmut

doi:10.1007/bf00019938

Cited by 27 publications

(16 citation statements)

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“…oleracea DNA in Southern blot hybridisations under high stringency conditions and when we compared this sequence with pBo1.6 similarity was found in only a small region (55 bp). No similarity was found between pBo1.6 and previous described repetitive sequences found in Brassica genomes (Benslimane et al, 1986;Reddy et al, 1989;Harbinder and Lakshmikumaran, 1990;Lakshmikumaran and Ranade, 1990;Gupta et al, 1990Gupta et al, , 1992Iwabuchi et al, 1991;Xia et al, 1993Xia et al, , 1994Harrison and Heslop-Harrison, 1995;Kapila et al, 1996b;Lim et al, 2005;Hong et al, 2006). As B .…”

Section: Discussionmentioning

confidence: 59%

Molecular characterisation and chromosomal localisation of a telomere-like repetitive DNA sequence highly enriched in the C genome of <i>Brassica</i>

Santos

Becker

Ecke

et al. 2007

Cytogenet Genome Res

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The aim of this work was to find C genome specific repetitive DNA sequences able to differentiate the homeologous A (B. rapa) and C (B. oleracea) genomes of Brassica, in order to assist in the physical identification of B. napus chromosomes. A repetitive sequence (pBo1.6) highly enriched in the C genome of Brassica was cloned from B. oleracea and its chromosomal organisation was investigated through fluorescent in situ hybridisation (FISH) in B. oleracea (2n = 18, CC), B. rapa (2n = 20, AA) and B. napus (2n = 38, AACC) genomes. The sequence was 203 bp long with a GC content of 48.3%. It showed up to 89% sequence identity with telomere-like DNA from many plant species. This repeat was clearly underrepresented in the A genome and the in situ hybridisation showed its B. oleracea specificity at the chromosomal level. Sequence pBo1.6 was localised at interstitial and/or telomeric/subtelomeric regions of all chromosomes from B. oleracea, whereas in B. rapa no signal was detected in most of the cells. In B. napus 18 to 24 chromosomes hybridised with pBo1.6. The discovery of a sequence highly enriched in the C genome of Brassica opens the opportunity for detailed studies regarding the subsequent evolution of DNA sequences in polyploid genomes. Moreover, pBo1.6 may be useful for the determination of the chromosomal location of transgenic DNA in genetically modified oilseed rape.

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Section: Discussionmentioning

confidence: 59%

Molecular characterisation and chromosomal localisation of a telomere-like repetitive DNA sequence highly enriched in the C genome of <i>Brassica</i>

Santos

Becker

Ecke

et al. 2007

Cytogenet Genome Res

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“…In centromere regions of maize (Alfenito and Birchler, 1993;Ananiev et al, 1998), pearl millet (Kamm et al, 1994), rice (Dong et al, 1998), sugarcane (Nagaki et al, 1998), sorghum (Zwick et al, 2000), the Australian daisy Brachycome dichromosomatica (Leach et al, 1995), rape (Xia et al, 1993), and the wild beet Beta procumbens (Gindullis et al, 2001), tandem repeats have been found that differ in sequence but that all have lengths in the range of ‫ف‬ 140 to 180 bp. Particular repeat arrays in cereals have been estimated to be hundreds of kilobases long (Dong et al, 1998;Kaszás and Birchler, 1998).…”

Section: Introductionmentioning

confidence: 99%

Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant

et al. 2002

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Centromeric H3-like histones, which replace histone H3 in the centromeric chromatin of animals and fungi, have not been reported in plants. We identified a histone H3 variant from Arabidopsis thaliana that encodes a centromere-identifying protein designated HTR12. By immunological detection, HTR12 localized at centromeres in both mitotic and meiotic cells. HTR12 signal revealed tissue-and stage-specific differences in centromere morphology, including a distended bead-like structure in interphase root tip cells. The anti-HTR12 antibody also detected spherical organelles in meiotic cells. Although the antibody does not label centromeres in the closely related species Arabidopsis arenosa , HTR12 signal was found on all centromeres in allopolyploids of these two species. Comparison of the HTR12 genes of A. thaliana and A. arenosa revealed striking adaptive evolution in the N-terminal tail of the protein, similar to the pattern seen in its counterpart in Drosophila . This finding suggests that the same evolutionary forces shape centromeric chromatin in both animals and plants. INTRODUCTIONCentromeres are the specialized chromosomal sites necessary for poleward movement during mitosis and meiosis in eukaryotes. Commonly, a centromere is evident as a prominent constriction within the heterochromatin of each metaphase chromosome. The attachment to and movement of chromosomes along the spindle is mediated by the proteinaceous kinetochores, which form at the centromeres during cell division.Despite this highly conserved function, centromeric DNA sequences are not conserved between organisms. For example, human centromeres consist of large blocks (200 kb to several megabases) of tandemly repeated 171-bp ␣ -satellite (Willard, 1998), but the sequences can differ from those of apes on homologous chromosomes (Haaf and Willard, 1997). Similarly, Drosophila melanogaster centromeric regions contain blocks of 5-to 12-bp satellite repeats that do not appear to be shared by homologous centromeres of sibling species (Lohe and Brutlag, 1987).Plant centromeric regions resemble their mammalian counterparts in that both have large arrays of tandem repeats, frequently of approximately nucleosomal size. In centromere regions of maize (Alfenito and Birchler, 1993;Ananiev et al., 1998), pearl millet (Kamm et al., 1994), rice (Dong et al., 1998), sugarcane (Nagaki et al., 1998), sorghum (Zwick et al., 2000), the Australian daisy Brachycome dichromosomatica (Leach et al., 1995), rape (Xia et al., 1993), and the wild beet Beta procumbens (Gindullis et al., 2001), tandem repeats have been found that differ in sequence but that all have lengths in the range of ‫ف‬ 140 to 180 bp. Particular repeat arrays in cereals have been estimated to be hundreds of kilobases long (Dong et al., 1998;Kaszás and Birchler, 1998). Cereal tandem repeat arrays are interrupted by TY3/gypsy -like retrotransposons and other retrotransposons (Ananiev et al., 1998;Kumekawa et al., 2001;Nonomura and Kurata, 2001). The satellite arrays and interspersed retrotransposons o...

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“…Twelve 2 Charon 4A clones, namely xlel, xle3, xle4, xle6, xle7, xle13, xle21, xle23, xle24, xle25, xle28 and xle29, were chosen for further analysis because they appeared to contain clusters of the canrep sequence that potentially were flanked by other types of DNA. The hybrid DNAs from these clones were digested with Eco RI, and the resultant fragments were subcloned in E. coliJM83r as inserts in the pUC18 and pUC19 [44] or pEMBL18 and pEMB L 19 [ 11 ] plasmids. According to the name of each original clone and the isolation number of each subclone, they were named xle4-7, xle6-14, xle7-2, xle13-11, xle21-2, xle21-16 and xle28-4.…”

Section: Subcloning and Genealogy Of Canrep-containing Dna Segmentsmentioning

confidence: 99%

Genomic organization of the canrep repetitive DNA in Brassica juncea

et al. 1994

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Canrep is a heterogeneous, tandemly repeated, 176 bp nucleotide sequence that contains a single Hind III site and is present in high copy numbers in the genomes of many Brassica species. Complete clusters of repeats of this DNA were cloned from the nuclear DNA of Brassica juncea. Restriction-fragment dimers and higher multimers of the 176 bp sequence have arisen by mutations within the Hind III recognition sequence. Adjacent repeats from within the same cluster usually have different nucleotide sequences with features indicating that diversity is generated by a mechanism that causes site-specific base substitutions. While most of the units of canrep DNA are clustered in long arrays of tandem repeats, some are dispersed throughout the genome as isolated copies or in small clusters. Regardless of the size of the arrays, each cluster begins and ends with a variable-length, truncated repeat and is flanked by inverted copies of the sequence 5'-ATCTCAT3'-, which is not part of the basic sequence of the canrep family of DNAs. Furthermore, some clusters are located close to nucleotide sequences related to those of known plant transposons. Thus, canrep elements may be dispersed by transposition. There are two distinct subfamilies of canrep sequences in B. juncea, and one of these is closely related to one of the two subfamilies of this type of DNA from B. napus, indicating that it originated from B. campestris, the common diploid ancestor of both amphidiploid species. Neither the repetitive DNA nor nucleotide sequences flanking canrep clusters are transcribed in seedlings, suggesting that even small arrays of repeats are located in heterochromatic regions and might be involved in chromatin condensation and/or chromosome segregation.

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Structure and evolution of a highly repetitive DNA sequence from Brassica napus

Cited by 27 publications

References 50 publications

Molecular characterisation and chromosomal localisation of a telomere-like repetitive DNA sequence highly enriched in the C genome of <i>Brassica</i>

Molecular characterisation and chromosomal localisation of a telomere-like repetitive DNA sequence highly enriched in the C genome of <i>Brassica</i>

Centromeric Localization and Adaptive Evolution of an Arabidopsis Histone H3 Variant

Genomic organization of the canrep repetitive DNA in Brassica juncea

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