Structure and expression analyses of the S-adenosylmethionine synthetase gene family in Arabidopsis thaliana

Peleman, J.; Saito, Kazuki; Cottyn, Bart; Engler, Gilbert; Seurinck, Jef; Montagu, Marc Van; Inzé, Dirk

doi:10.1016/0378-1119(89)90510-6

Cited by 98 publications

(80 citation statements)

References 26 publications

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“…SAM synthetases have been extensively studied in bacteria, yeasts, plants and mammals including humans (Cai et al, 1996;Peleman et al, 1989;Porcelli et al, 1988;Sakata et al, 1993;Thomas and Surdin-Kerjan, 1987), but less is known about them in filamentous fungi. SAM synthetases are highly conserved between bacteria and eukaryotes, with lower conservation in archaea (Graham et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Gerke

Bayram

Braus

2012

Fungal Genetics and Biology

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a b s t r a c tThe filamentous fungus Aspergillus nidulans carries a single gene for the S-adenosylmethionine (SAM) synthetase SasA, whereas many other organisms possess multiple SAM synthetases. The conserved enzyme catalyzes the reaction of methionine and ATP to the ubiquitous methyl group donor SAM. SAM is the main methyl group donor for methyltransferases to modify DNA, RNA, protein, metabolites, or phospholipid target substrates. We show here that the single A. nidulans SAM synthetase encoding gene sasA is essential. Overexpression of sasA, encoding a predominantly cytoplasmic protein, led to impaired development including only small sterile fruiting bodies which are surrounded by unusually pigmented auxiliary Hülle cells. Hülle cells are the only fungal cell type which does not contain significant amounts of SasA. Sterigmatocystin production is altered when sasA is overexpressed, suggesting defects in coordination of development and secondary metabolism. SasA interacts with various metabolic proteins including methionine or mitochondrial metabolic enzymes as well as proteins involved in fungal morphogenesis. SasA interaction to histone-2B might reflect a putative epigenetic link to gene expression. Our data suggest a distinct role of SasA in coordinating fungal secondary metabolism and development.

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Section: Introductionmentioning

confidence: 99%

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Gerke

Bayram

Braus

2012

Fungal Genetics and Biology

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“…Previous reports have described the expression of SAMS-GUS reporter genes in roots, stems, and leaves of Arabidopsis (Peleman et al, 1989a) and tobacco (Peleman et al, 1989b) and in leaves of rice (Dekeysen et al, 1990). We determined the spatial distribution of SAMS genes at different stages of ovary development and senescence.…”

Section: Spatial Distribution Of Sams Genes In Pea Ovariesmentioning

confidence: 99%

“…In all of the characterized systems, a multigene family encodes SAMS enzymes. The existence of different SAMS genes has generally been ascribed to the metabolic importance of SAM (Thomas and Surdin-Kerjan, 1987;Peleman et al, 1989b). Moreover, it has been suggested that some of the SAMS genes would be expressed constitutively, whereas others would be specifically regulated by developmental and/or environmental factors strictly controlled according to the requirement for SAM (Boerjan et al, 1994).…”

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confidence: 99%

Differential Expression of theS-Adenosyl-l-Methionine Synthase Genes during Pea Development1

Gómez-Gómez

Carrasco

1998

Plant Physiology

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Two genes coding for S-adenosyl-L-methionine synthase (SAMS, EC 2.5.1.6) were previously isolated from pea (Pisum sativum) ovaries. Both SAMS genes were highly homologous throughout their coding regions but showed a certain degree of sequence divergence within the 5 and the 3 untranslated regions. These regions have been used as gene-specific probes to analyze the differential expression of SAMS1 and SAMS2 genes in pea plants. The ribonuclease protection assay revealed different expression patterns for each individual gene. SAMS1 was strongly expressed in nearly all tissues, especially in roots. SAMS2 expression was weaker, reaching its highest level at the apex. Following pollination, SAMS1 was specifically up-regulated, whereas SAMS2 was expressed constitutively. The up-regulation of SAMS1 during ovary development was also observed in unpollinated ovaries treated with auxins. In unpollinated ovaries an increase in SAMS1 expression was observed as a consequence of ethylene production associated with the emasculation process. In senescing ovaries both SAMS1 and SAMS2 genes showed increased expression. Ethylene treatment of unpollinated ovaries led to an increase in the SAMS1 mRNA level. However, SAMS2 expression remained unchangeable after ethylene treatment, indicating that SAMS2 induction during ovary senescence was not ethylene dependent. SAMS mRNAs were localized by in situ hybridization at the endocarp of developing fruits and in the ovules of senescing ovaries. Our results indicate that the transcriptional regulation of SAMS genes is developmentally controlled in a specific way for each gene.

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“…In plants, SAM also functions as a precursor in the biosynthesis of the phytohormone ethylene (Yang and Hoffman, 1984). SAM-S genomic and cDNA clones have been isolated from several plant species, including Arabidopsis tkaliana, carnation, parsley, poplar, and rice (van Breusegem et al, 1994). The saml gene of A .…”

mentioning

confidence: 99%

“…The level of expression of this gene in stems and roots is 10 to 20 times higher than in leaves or flowers (Peleman et al, 1989b). In rice, a s i m i l a r level of sam expression has been found in leaves and roots (van Breusegem et al, 1994). In parsley leaves, a funga1 elicitor induces the expression of the gene (Kawalleck et al, 1992).…”

mentioning

confidence: 99%

A Petunia cDNA Encoding S-Adenosylmethionine Synthetase

Izhaki

Shoseyov²,

Weiss³

1995

Plant Physiol.

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Structure and expression analyses of the S-adenosylmethionine synthetase gene family in Arabidopsis thaliana

Cited by 98 publications

References 26 publications

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Differential Expression of theS-Adenosyl-l-Methionine Synthase Genes during Pea Development1

A Petunia cDNA Encoding S-Adenosylmethionine Synthetase

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