Structure and expression of class I MHC genes in the miniature swine

Singer, Dinah S.; Ehrlich, Rachel; Satz, L.; Frels, William I.; Bluestone, Jeffrey A.; Hodes, Richard J.; Rudikoff, Stuart

doi:10.1016/0165-2427(87)90141-3

Cited by 31 publications

(16 citation statements)

References 22 publications

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“…Assuming that one cM represents -1 Mb, the data suggest that the SEA is in the range of I-2 million bp, smaller than the MHC of humans (3.5-5 Mb; Carroll et al 1987;Dunham et al 1987;Lawrance et al 1987). The smaller apparent size of the SLA is within the 2000 kb estimated by PFGE (Xu et al 1992) and may be attributable, in part, to the lower number of class I genes found in swine (Singer et al 1988).…”

Section: Linkage Analysismentioning

confidence: 87%

“…The low resolution of these assignments could result from a dispersed distribution of SLA genes that crosshybridize with the selected probe; however, a limited physical map of the SLA produced via pulsed field gel electrophoresis (PFGE) suggests the complex is -2 Mb in width (Xu et al 1992). Although swine appear to have fewer class I genes than other mammalian species studied, at least seven genes hybridized to an HLA probe (Singer et al 1988), which may also affect the resolution of the physical assignments. A more recent analysis using a radiolabeled class III gene [tumor necrosis factor ~ (TNFcz); Kuhnert et al 1991] that appears to be single copy in the swine genome reported an assignment to 7ql.…”

Section: T Smith Et Almentioning

confidence: 99%

“…Subsequent analysis using the same probe (Echard et al 1986) or a full-length porcine class I gene probe (PDIA; Rabin et al 1985;Singer et al 1988) reported the SLA to lie near the centromere in the region 7p1.4-q1.2 and 7p1.2-q1.2, respectively. The low resolution of these assignments could result from a dispersed distribution of SLA genes that crosshybridize with the selected probe; however, a limited physical map of the SLA produced via pulsed field gel electrophoresis (PFGE) suggests the complex is -2 Mb in width (Xu et al 1992).…”

Section: T Smith Et Almentioning

confidence: 99%

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Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere.

Smith¹,

Rohrer²,

Alexander³

et al. 1995

Genome Res.

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The first integrated physical and genetic linkage map encompassing the entire swine chromosome 7 (SSC7) reveals that the porcine MHC (SLA) spans the centromere. A SLA class !I antigen gene lies on the q arm, whereas class I and !!1 genes lie on the p arm, suggesting that the presence of a centromere within the SLA does not preclude a functional complex. The SLA appears smaller than other mammalian MHC, as the genetic distance across two class !, three class !1, and three class !!i SLA gene markers is only I.I cM. There are significant variations in recombination rates as a function of position along the chromosome, and the SLA lies in the region with the lowest rate. Furthermore, the directed integration approach used in this study was more efficient than previous efforts that emphasized the screening of large insert libraries for random microsatellites.Genetic maps of livestock species are currently being developed to provide a worldwide resource for mapping quantitative trait loci, comparative mapping in evolutionary studies, and mapping of pertinent loci in animal models of human disorders. These objectives require a framework of informative markers that can be genotyped economically. Integration of this framework with a cytogenetic map allows evaluation of coverage, genome size, and recombination rates along the chromosome, as well as establishing syntenic relationships among species. Two linkage maps of swine chromosome 7 (SSC7) have been reported, one that includes 11 Archibald et al. 1995) and one with 17 ) microsatellite markers. The two maps have only one marker in common, and both contain intervals >30 cM. The cytogenetic map includes only four relatively low resolution assignments of linked markers with -30% coverage (Ed-

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Section: Linkage Analysismentioning

confidence: 87%

Section: T Smith Et Almentioning

confidence: 99%

Section: T Smith Et Almentioning

confidence: 99%

See 1 more Smart Citation

Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere.

Smith¹,

Rohrer²,

Alexander³

et al. 1995

Genome Res.

View full text Add to dashboard Cite

show abstract

“…Seven SLA class I genes have been estimated to exist in the porcine genome (Singer et al 1988) and several have been characterized in detail at the levels of DNA structure, expression and regulation (Ehrlich et al 1987;Satz et al 1985).…”

Section: Discussionmentioning

confidence: 98%

“…The major histocompatibility complex of the pig has been studied to better understand the genetic associations between health and production and the SLA Downloaded by [University of Florida] at 07:37 01 June 2016 complex Chardon et al 1988;Singer et al 1988). Seven SLA class I genes have been estimated to exist in the porcine genome (Singer et al 1988) and several have been characterized in detail at the levels of DNA structure, expression and regulation (Ehrlich et al 1987;Satz et al 1985).…”

Section: Discussionmentioning

confidence: 99%

RFLP analysis of SLA haplotypes in Swiss large white and American Hampshire pigs using SLA class I and class II probes

Shia

Gautschi

Ling

et al. 1991

Animal Biotechnology

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Restriction fragment length polymorphism (RFLP) analyses of swine leukocyte antigen (SLA) class I and class II genes from Swiss Large White and American Hampshire families were performed using porcine DNA probes. Class I and class II RFLPs associated with the serologically-defined haplotypes SLA H1, H8, H16 and H24 and with serotypes SLA 15, 16; SLA 14; and SLA 6, SB 19, were identified. Seven allelic class I RFLP patterns were observed. For genes in the SLA class II region, six allelic RFLP patterns of DQA and DQB; five allelic RFLP patterns of DRA; and seven allelic RFLP patterns of DRB were observed. The serologicallydefined H8 haplotype was subtyped based on differences in class II RFLPs.

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Collection of blood mononuclear cells by leukapheresis for transplantation in a yucatan miniature swine model

Smith

Stribley

Lieberman

et al. 1992

J of Clinical Apheresis

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A large animal model is needed to evaluate new apheresis technologies. These technologies include novel methods of harvesting the blood mononuclear cell population which contains the hematopoietic stem cells needed to restore hematopoiesis in recipients of hematopoietically lethal therapy and the use of cytokines to provide a safe and predictable method of manipulating these circulating hematopoietic stem cells. We describe the methods used to collect mononuclear cells by leukapheresis from Yucatan miniature swine. These animals are of sufficient size to tolerate the procedures and have many physiologic and hematologic similarities to man. They are of good temperament and are easily trained. Long-term venous access was obtained using single lumen silicone rubber catheters placed in the inferior vena cava. The animals were apheresed while fully awake using a Haemonetics Model V50 machine and a modified lymphocyte collection protocol. The procedure was highly efficient for the collection of mononuclear cells and a 10 pass procedure yielded a product which contained 19.7 x 10(9) mononuclear cells, 10.7 x 10(9) granulocytes, and 17 ml of erythrocytes in a volume of approximately 100 ml. This product can be cryopreserved and used for subsequent transplantation. The content of four apheresis procedures provides hematopoietic reconstitution of lethally irradiated swine on a time scale equivalent to transplantation of optimal numbers of bone marrow cells.

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Structure and expression of class I MHC genes in the miniature swine

Cited by 31 publications

References 22 publications

Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere.

Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere.

RFLP analysis of SLA haplotypes in Swiss large white and American Hampshire pigs using SLA class I and class II probes

Collection of blood mononuclear cells by leukapheresis for transplantation in a yucatan miniature swine model

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