Structure and expression of cloned murine IFN-α genes

Shaw, Gray D.; Boll, Werner; Taira, Hideharu; Mantei, Ned; Lengye, Peter; Weissmann, Charles

doi:10.1093/nar/11.3.555

Cited by 154 publications

(80 citation statements)

References 59 publications

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“…It has been shown by Southern blot analysis of total mouse DNA hybridized with a HulFN-c~ probe that at least 10 different IFN-ct genes and pseudogenes are present in the mouse genome (Owerbach et al, 1981 ;Shaw et al, 1983). Two of those genes have been isolated and sequenced (Shaw et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…Two of those genes have been isolated and sequenced (Shaw et al, 1983). We have recently isolated six MulFN-~ genes (E. C. Zwarthoff et al, unpublished results).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, there are indications from experiments involving treatment of interferon with glycosidases or synthesis of interferon in the presence of tunicamycin that MuIFN-ct is glycosylated (Fujisawa et al, 1978;Fujisawa & Kawade, 1981). Also, the amino acid sequences as deduced from the two MulFN-ct nucleotide sequences known so far show the presence of one possible N-glycosylation site (Shaw et al, 1983). So, heterogeneity in glycosylation may also account for the heterogeneity in charge of MulFN-~ described in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Although the presence of several IFN-~ genes in the mouse genome has been described (Owerbach et al, 1981 ;Shaw et al, 1983) no data are available, as yet, concerning the properties of MulFN-c~ protein subspecies. In this study we describe a method for the separation of MulFN-c~ into subspecies and a further characterization of these subspecies.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation and Characterization of Subspecies of Murine Interferon Alpha

Lemson¹,

Vonk²,

Korput³

et al. 1984

Journal of General Virology

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SUMMARYInterferon produced by mouse L-929 cells by incubation with poly(rI), poty(rC) is known to be composed of a mixture of MuIFN-c~ and MuIFN-fl. The c~ component was separated from the fl species by affinity chromatography over a monoclonal antiMuIFN-fl agarose column and partially purified by gel filtration. MuIFN-c~, prepared by this method was separated into at least five subspecies by chromatofocusing. The approximate pI values of these components are >~ 7.5, 6.5, 6.2, 5-9 and 5.6, respectively. Component 3 (pI 6.2) was the most prominent subspecies present in our MulFN-~ preparations, representing 40 to 50 ~o of the total antiviral activity. Component 1 (pI /> 7.5) which accounted for about 5~ of the antiviral activity on mouse cells, differed in some properties from the other interferon subspecies. It showed a relatively high antiviral activity on heterologous cells and it was eluted from a Sephadex column after the other c¢ subspecies. Furthermore, it showed a diminished binding to heparin as compared to the other MulFN-c¢ subspecies, indicating a lower affinity for polynucleotides.

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Section: Discussionmentioning

confidence: 99%

“…Two of those genes have been isolated and sequenced (Shaw et al, 1983). We have recently isolated six MulFN-~ genes (E. C. Zwarthoff et al, unpublished results).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Characterization of Subspecies of Murine Interferon Alpha

Lemson¹,

Vonk²,

Korput³

et al. 1984

Journal of General Virology

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“…The LXSN retroviral vector 15 16 tailored with BamHI linkers, into the unique BamHI site of the LXSN retroviral vector, containing the neomycin resistance gene under the control of the simian virus 40 promoter. In the resulting construct, the IFN complementary DNA is under the transcriptional control of the 5Ј retroviral long terminal repeat.…”

Section: Retroviral Vector Production and Transduction Of Esb Tumor Cmentioning

confidence: 99%

Murine interferon-α1 gene-transduced ESb tumor cells are rejected by host-mediated mechanisms despite resistance of the parental tumor to interferon-α/β therapy

et al. 1999

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The highly metastatic ESb tumor is totally resistant to murine interferon-␣/␤ (IFN-␣/␤) therapy, regardless of the number of cells injected or the route of inoculation. In contrast, as we show herein, mouse IFN-␣ 1 -transduced ESb tumor cells were inhibited markedly when injected subcutaneously into immunocompetent mice. IFN-producing ESb tumor rejection was mediated by the immune system, because these tumor cells grew normally in immunosuppressed mice. Tumor regression was accompanied by extensive necrosis and cellular infiltrates in the tumor area. These results further support the use of IFN-␣ in cytokine gene therapy of cancer and suggest the advantage of using gene transfer rather than cytokine administration to enhance an antitumor immune response.

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Heterogeneity and functions of the 13 IFN‐α subtypes – lucky for some?

et al. 2023

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Type I IFNs are critical for host responses to viral infection and are also implicated in the pathogenesis of multiple autoimmune diseases. Multiple subtypes exist within the type I IFN family, in particular 13 distinct IFN‐α genes, which signal through the same heterodimer receptor that is ubiquitously expressed by mammalian cells. Both evolutionary genetic studies and functional antiviral assays strongly suggest differential functions and activity between the 13 IFN‐α subtypes, yet we still lack a clear understanding of these different roles. This review summarizes the evidence from studies describing differential functions of IFN‐α subtypes and highlights potential reasons for discrepancies between the reports. We examine both acute and chronic viral infection, as well as autoimmunity, and integrate a more recent awareness of the importance of anti‐IFN‐α autoantibodies in shaping the type I IFN responses in these different conditions.

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Structure and expression of cloned murine IFN-α genes

Cited by 154 publications

References 59 publications

Isolation and Characterization of Subspecies of Murine Interferon Alpha

Isolation and Characterization of Subspecies of Murine Interferon Alpha

Murine interferon-α1 gene-transduced ESb tumor cells are rejected by host-mediated mechanisms despite resistance of the parental tumor to interferon-α/β therapy

Heterogeneity and functions of the 13 IFN‐α subtypes – lucky for some?

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