Structure and expression of the bovine amelogenin gene

Abstract

In order to define further the mechanisms responsible for tooth amelogenin heterogeneity, seven bovine amelogenin cDNAs were sequenced. On the basis of these sequences, five of the cDNAs could be grouped into one class which differed appreciably in sequence from the second group of two cDNAs. Two overlapping bovine genomic clones were then isolated and shown by sequencing to contain six exons encoding the entire consensus sequence of the class I cDNA. Southern blot analysis of DNA from male and female animals using class I or class II specific oligonucleotide probes suggested that the class I gene sequence was located on the X chromosome while the class II sequence was located on the Y chromosome. Therefore, these results also suggest that the genes on the X and Y chromosomes are both transcribed. Furthermore, the results are consistent with alternative splicing of the class I primary transcript as a potential mechanism for generating amelogenin heterogeneity.

Regulation of amelogenin gene expression during tooth development

“…This location suggests that our longest cDNA clone previously described (Gibson et al, 1991) lacked 20 bp a t the 5' end ( Fig. 3).…”

“…A clone was isolated from a Charon 30 bovine genomic library that contains the translation initiation site in exon 2, plus approximately 11.5 kb of upstream sequences (Gibson, et al, 1991). A 7 kb Hind111 frag- The DNA sequence was determined for approximately 950 bp upstream from exon 1.…”

“…A 7 kb Hind111 frag- The DNA sequence was determined for approximately 950 bp upstream from exon 1. The 5' end of the previously cloned X-chromosomal bovine amelogenin cDNA (Gibson et al, 1991) was determined to be approximately 50 bp downstream from a TATA box, but this element is usually found approximately 30 bp from the site of transcription initiation (Breathnach and Chambon, 1981). An inverted CCAAT box was found upstream from the TATA box (Fig.…”

“…Recombinant Charon 30 phage clones Baml and Bam2 previously isolated from a bovine genomic library contain the entire bovine X-chromosomal amelogenin gene (Gibson et al, 1991). A 7.0 kb Hind111 fragment of this clone, which includes part of intron 1, exon 1, and upstream sequences, was subcloned into pUC19.…”

Specific Amelogenin Gene Splice Products Have Signaling Effects on Cells in Culture and in Implants in Vivo

“…The amino-terminal sequence and one internal tryptic peptide sequence were determined. Both sequences proved to be derived from the aminoterminal portion of bovine amelogenin (11,12). This was a surprising result for two reasons.…”