Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana

Koizumi, Masahiro; Yamaguchi-Shinozaki, Kazuko; Tsuji, Hideo; Shinozaki, Kazuo

doi:10.1016/0378-1119(93)90266-6

Cited by 272 publications

(186 citation statements)

References 17 publications

Supporting

Mentioning

175

Contrasting

Unclassified

Order By: Relevance

“…In15 and In21 (putative cysteine proteases) were similar to PD19 and RD21 of Arabidopsis. The expression of these two genes was induced by drought stress (Koizumi et al 1993), but it is not clear if this occurred independently of water-stress induced senescence. (Yamada et al 2006) In29 encoded a putative leucine-rich repeat transmembrane receptor protein kinase.…”

Section: Appearance Of Senescence Parameters Compared With Gene Exprementioning

confidence: 99%

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

et al. 2007

View full text Add to dashboard Cite

We isolated several senescence-associated genes (SAGs) from the petals of morning glory (Ipomoea nil) flowers, with the aim of furthering our understanding of programmed cell death. Samples were taken from the closed bud stage to advanced visible senescence. Actinomycin D, an inhibitor of transcription, if given prior to 4 h after opening, suppressed the onset of visible senescence, which occurred at about 9 h after flower opening. The isolated genes all showed upregulation. Two cell-wall related genes were upregulated early, one encoding an extensin and one a caffeoyl-CoA-3-O-methyltransferase, involved in lignin production. A pectinacetylesterase was upregulated after flower opening and might be involved in cell-wall degradation. Some identified genes showed high homology with published SAGs possibly involved in remobilisation processes: an alcohol dehydrogenase and three cysteine proteases. One transcript encoded a leucine-rich repeat receptor protein kinase, putatively involved in signal transduction. Another transcript encoded a 14-3-3 protein, also a protein kinase. Two genes have apparently not been associated previously with senescence: the first encoded a putative SEC14, which is required for Golgi vesicle transport, the second was a putative ataxin-2, which has been related to RNA metabolism. Induction of the latter has been shown to result in cell death in yeast, due to defects in actin filament formation. The possible roles of these genes in programmed cell death are discussed.

show abstract

Section: Appearance Of Senescence Parameters Compared With Gene Exprementioning

confidence: 99%

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

et al. 2007

View full text Add to dashboard Cite

show abstract

“…1). The rd21 gene has been shown to be up-regulated during dehydration (Koizumi et al, 1993). To clarify an accumulation of RD21 at the protein level, we raised antibodies against proRD21 and the granulin domain (Fig.…”

Section: Arabidopsis Leaves Accumulate Not Only the Mature Protein Ofmentioning

confidence: 99%

“…An rd21 gene encoding a papain family protease, RD21 (D13043), was found to be up-regulated during dehydration of Arabidopsis plants (Koizumi et al, 1993). Papain family proteases have NTPP that includes an ERFNIN motif, which is conserved in rat cathepsin H (Y00708), cathepsin L (Y00697), and human cathepsin S (Bromme et al, 1993;Karrer et al, 1993).…”

mentioning

confidence: 99%

A Slow Maturation of a Cysteine Protease with a Granulin Domain in the Vacuoles of Senescing Arabidopsis Leaves

et al. 2001

View full text Add to dashboard Cite

Arabidopsis RD21 is a cysteine protease of the papain family. Unlike other members of the papain family, RD21 has a C-terminal extension sequence composed of two domains, a 2-kD proline-rich domain and a 10-kD domain homologous to animal epithelin/granulin family proteins. The RD21 protein was accumulated as 38-and 33-kD proteins in Arabidopsis leaves. An immunoblot showed that the 38-kD protein had the granulin domain, whereas the 33-kD protein did not. A pulse-chase experiment with Bright-Yellow 2 transformant cells expressing RD21 showed that RD21 was synthesized as a 57-kD precursor and was then slowly processed to make the 33-kD mature protein via the 38-kD intermediate. After a 12-h chase, the 38-kD intermediate was still detected in the cells. These results indicate that the N-terminal propeptide was first removed from the 57-kD precursor, and the C-terminal granulin domain was then slowly removed to yield the 33-kD mature protein. Subcellular fractionation of the Bright-Yellow 2 transformant showed that the intermediate and mature forms of RD21 were localized in the vacuoles. Under the acidic conditions of the vacuolar interior, the intermediate was found to be easily aggregated. The intermediate and the mature protein were accumulated in association with leaf senescence. Taken together, these results indicate that the intermediate of RD21 was accumulated in the vacuoles as an aggregate, and then slowly matured to make a soluble protease by removing the granulin domain during leaf senescence.

show abstract

“…Interestingly, DRE and G-box related to drought stress and ABA response (Yamaguchi-Shinozaki et al 1995) were found in the promoter region of CP2. The amino acid sequence of CP2 is similar to drought-inducible cysteine proteinases, Arabidopsis RD19 (Koizumi et al 1993) and pea 15a (Guerrero et al 1990). These facts suggest that drought stress may activate CP2.…”

mentioning

confidence: 96%

Hormonal regulation of the expression of cysteine proteinase genes in germinated cotyledons of common bean seeds

Yamauchi

2007

Plant Biotechnology

View full text Add to dashboard Cite

Storage proteins in cotyledons of legume plants are degraded by proteinases after germination. We examined whether abscisic acid (ABA), gibberellin (GA) and brassinosteroid (BR) are involved in the expression of cysteine proteinases in cotyledons of common bean seeds with RNA blotting using cDNAs for five papain-like proteinases, EP-C1, CP1, CP2, CP3, CP4 and two legumain-like proteinases, LLP1 and LLP2 as probes. Six genes, except for CP4, are activated after seed imbibition. These germination-induced cysteine proteinase genes (GICPs) are repressed by exogenously applied (ϩ)-5Јa, 8Ј-cyclo-ABA which is more resistant to hydroxylation than ABA. Either GA 3 or epi-BR activated GICPs in the presence of ABA, suggesting that both of GA and BR are involved in the expression of GICPs. It is possible that GA biosynthesis is required for the activation of GICPs, because either GA-biosynthesis inhibitors, chloroethyltrimethylammonium chloride or prohexadione calcium repressed GICPs and this repression was restored by exogenously applied GA 3 .

show abstract

Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana

Cited by 272 publications

References 17 publications

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

A Slow Maturation of a Cysteine Protease with a Granulin Domain in the Vacuoles of Senescing Arabidopsis Leaves

Hormonal regulation of the expression of cysteine proteinase genes in germinated cotyledons of common bean seeds

Contact Info

Product

Resources

About