2001
DOI: 10.1091/mbc.12.1.85
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Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells

Abstract: The ␣4 laminin subunit is a component of endothelial cell basement membranes. An antibody (2A3) against the ␣4 laminin G domain stains focal contact-like structures in transformed and primary microvascular endothelial cells (TrHBMECs and HMVECs, respectively), provided the latter cells are activated with growth factors. The 2A3 antibody staining colocalizes with that generated by ␣v and ␤3 integrin antibodies and, consistent with this localization, TrHBMECs and HMVECs adhere to the ␣4 laminin subunit G domain … Show more

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Cited by 148 publications
(144 citation statements)
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“…The VIM disassembly implies its phosphorylation (Chan et al, 2002) that positively mediates cell adhesion, motility and signaling. Accordingly, we showed that the VIM knockdown inhibits MMECs spreading and migration, similarly to VIM-silenced fibroblasts and epithelial cells (Gilles et al, 1999) and ECs in which adhesion (Gonzales et al, 2001) and formation of focal contacts (Tsuruta and Jones, 2003) are halted. Moreover, VIM is overexpressed in rat ECs with migrating phenotype versus cells with low migratory activity (Obermeyer et al, 2003).…”
Section: Angiogenic Proteins Of Mmecs S Berardi Et Alsupporting
confidence: 51%
“…The VIM disassembly implies its phosphorylation (Chan et al, 2002) that positively mediates cell adhesion, motility and signaling. Accordingly, we showed that the VIM knockdown inhibits MMECs spreading and migration, similarly to VIM-silenced fibroblasts and epithelial cells (Gilles et al, 1999) and ECs in which adhesion (Gonzales et al, 2001) and formation of focal contacts (Tsuruta and Jones, 2003) are halted. Moreover, VIM is overexpressed in rat ECs with migrating phenotype versus cells with low migratory activity (Obermeyer et al, 2003).…”
Section: Angiogenic Proteins Of Mmecs S Berardi Et Alsupporting
confidence: 51%
“…Lastly, WFA has vimentin-dependent, anti-angiogenic activity at doses that are 500 fold lower than doses showing anti-tumor activity. 21,24,25 Therefore, it is possible that this anti-angiogenic activity is due to inhibition of vimentin during endothelial cell motility, 48,49 though in our in vivo model we did not observe reduced angiogenesis with this treatment schedule and dosing (data not shown).…”
Section: Cancer Therapymentioning
confidence: 67%
“…Interestingly, the assembly of at least some keratin IF from their precursors appears to be associated with focal adhesions located in lamellipodia of epithelial cells [25] (••). There is also evidence that vimentin IF associate with focal adhesions and can modulate those associated with αvβ3 integrin in endothelial cells [26]. Mechanical stress enhances the association of bundles of vimentin with focal contacts, altering their dynamic properties [27], and silencing vimentin expression results in smaller focal contacts and decreased adhesion [27].…”
Section: (••)mentioning
confidence: 99%