Structure and Function of Actin Filaments in Mature Guard Cells

Hwang, Jae‐Ung; Eun, Soon-Ok; Lee, Youngsook

doi:10.1007/978-94-015-9460-8_24

Cited by 20 publications

(34 citation statements)

References 25 publications

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“…It is widely accepted that, in elliptical stomata, cortical AFs are essential in the mechanism of opening/ closing of the stomatal pore [reviewed by Hwang et al, 2000;Duqué et al, 2004;Galatis and Apostolakos, 2004]. In contrast, our structural and experimental data suggest that, in the dumbbell-shaped stomata of Zea mays, cortical AFs are not involved in the above mechanism.…”

Section: Opening and Closing Of Grass Stomata Does Not Depend On Afs contrasting

confidence: 82%

Cortical actin filament organization in developing and functioning stomatal complexes of Zea mays and Triticum turgidum

Panteris

Galatis

Quader

et al. 2007

Cell Motil. Cytoskeleton

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Cortical actin filament (AF) organization was studied in detail in developing stomatal complexes of the grasses Zea mays and Triticum turgidum. AF arrays during the whole stomatal complex development are dynamic, partly following the pattern of cortical microtubule (MT) organization. They also exhibit particular patterns of organization, spatially and temporarily restricted. Among AF arrays, the radial ones that underlie young guard cell (GC) periclinal walls, those that line the bulbous GC ends and the AF ring at the junction between subsidiary cells (SCs) and GCs are described here for the first time. Although many similarities in cortical AF organization exist among the stomatal cells of both plants studied, considerable differences have also been observed between them. Our data reveal that the expanding areas of stomatal cell walls are lined by distinct cortical AF aggregations that probably protect the plasmalemma against mechanical stresses. Experimental AF disruption does not seem to affect detectably stomatal cell morphogenesis. Moreover, the structural and experimental data of this study revealed that, in contrast to the elliptical stomata, in the dumbbell-shaped ones the AFs and MTs seem not to be involved in the mechanism of opening and closing of the stomatal pore.

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Section: Opening and Closing Of Grass Stomata Does Not Depend On Afs contrasting

confidence: 82%

Cortical actin filament organization in developing and functioning stomatal complexes of Zea mays and Triticum turgidum

Panteris

Galatis

Quader

et al. 2007

Cell Motil. Cytoskeleton

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“…To investigate further this phenomenon, the pattern of callose deposition in CB‐affected stomata was investigated. In the elliptical stomata, actin filament disorganization by cytochalasins induces an exceptional increase of the stomatal pore width (Hwang et al. , 2000).…”

Section: Resultsmentioning

confidence: 99%

“…Unlike the untreated opened stomata of A. nidus , those treated with CB or cellulose synthesis inhibitors (coumarin and dichlobenil) form abundant callose in their periclinal walls. In elliptical stomata, actin filament disintegration induces a detectable increase in the width of the stomatal pore (Hwang et al. , 2000, and literature therein), a phenomenon also confirmed in A. nidus stomata (Fig.…”

Section: Discussionmentioning

confidence: 99%

Callose implication in stomatal opening and closure in the fern Asplenium nidus

et al. 2010

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Summary• The involvement of callose in the mechanism of stomatal pore opening and closing in the fern Asplenium nidus was investigated by examination of the pattern of callose deposition in open and closed stomata, and by examination of the effects of callose degradation and inhibition or induction of callose synthesis in stomatal movement.• Callose was identified with aniline blue staining and a callose antibody and degraded via b-1,3-D-glucanase. Callose synthesis was inhibited with 2-deoxy-Dglucose and induced by coumarin or dichlobenil. Stomatal pore opening and closing were assessed by estimation of the stomatal pore width.• The open stomata entirely lacked callose, while the closed ones displayed distinct radial fibrillar callose arrays in the external periclinal walls. The latter displayed local bending at the region of callose deposition, a deformation that was absent in the open stomata. Both callose degradation and inhibition of callose synthesis reduced the stomatal ability to open in white light and close in darkness. By contrast, callose synthesis induction considerably improved stomatal pore opening and reduced stomatal closure in same conditions.• The present data revealed that: during stomatal closure the external periclinal guard cell walls experience a strong mechanical stress, probably triggering callose synthesis; and that callose participates in stomatal movement.

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“…A growing body of data indicates that dynamic AF structures also correlate with stomatal movement (Kim et al . 1995; Eun & Lee 1997, 2000; Huang, Wang & Lou 2000; Hwang, Eun & Lee 2000; Choi et al . 2008; Gao et al .…”

Section: Introductionmentioning

confidence: 99%