Structure and Function of Bacteriophages

Sanz-Gaitero, Marta; Seoane-Blanco, Mateo; Raaij, M.J. van

doi:10.1007/978-3-319-41986-2_1

Cited by 13 publications

(24 citation statements)

References 181 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2 b). The angular characteristic observed for small phage particles became noticeable for the C22 phage 43 , 44 . No structural damage or disintegration of the phage particles was observed upon a close look at the phage structure at four temperatures (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 92%

Thermoresponsive C22 phage stiffness modulates the phage infectivity

Sae-Ueng

Bhunchoth

Phironrit

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Bacteriophages offer a sustainable alternative for controlling crop disease. However, the lack of knowledge on phage infection mechanisms makes phage-based biological control varying and ineffective. In this work, we interrogated the temperature dependence of the infection and thermo-responsive behavior of the C22 phage. This soilborne podovirus is capable of lysing Ralstonia solanacearum, causing bacterial wilt disease. We revealed that the C22 phage could better infect the pathogenic host cell when incubated at low temperatures (25, 30 °C) than at high temperatures (35, 40 °C). Measurement of the C22 phage stiffness revealed that the phage stiffness at low temperatures was 2–3 times larger than at high temperatures. In addition, the imaging results showed that more C22 phage particles were attached to the cell surface at low temperatures than at high temperatures, associating the phage stiffness and the phage attachment. The result suggests that the structure and stiffness modulation in response to temperature change improve infection, providing mechanistic insight into the C22 phage lytic cycle. Our study signifies the need to understand phage responses to the fluctuating environment for effective phage-based biocontrol implementation.

show abstract

Section: Resultsmentioning

confidence: 92%

Thermoresponsive C22 phage stiffness modulates the phage infectivity

Sae-Ueng

Bhunchoth

Phironrit

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The 40,425 bp pphBh CH20 constitutes the third different type of mobile genetic element described so far in B. hyodysenteriae . It resembles the structure of the tailed and double-stranded DNA Streptococcus agalactiae phage LYGO9 (JX409894) of the Siphoviridae family [ 61 ]. In line with this, the lnu (C)-carrying transposon MTn Sag 1 recently detected in B. hyodysenteriae , was originally identified in S. agalactiae [ 11 ], demonstrating the capability of B. hyodysenteriae to acquire foreign genetic material from other bacterial species through various HGT mechanisms [ 4 , 5 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-genome analyses reveal a novel prophage and cgSNPs-derived sublineages of Brachyspira hyodysenteriae ST196

et al. 2022

View full text Add to dashboard Cite

Background Brachyspira (B.) hyodysenteriae is a fastidious anaerobe spirochete that can cause swine dysentery, a severe mucohaemorragic colitis that affects pig production and animal welfare worldwide. In Switzerland, the population of B. hyodysenteriae is characterized by the predominance of macrolide-lincosamide-resistant B. hyodysenteriae isolates of sequence type (ST) ST196, prompting us to obtain deeper insights into the genomic structure and variability of ST196 using pangenome and whole genome variant analyses. Results The draft genome of 14 B. hyodysenteriae isolates of ST196, sampled during a 7-year period from geographically distant pig herds, was obtained by whole-genome sequencing (WGS) and compared to the complete genome of the B. hyodysenteriae isolate Bh743-7 of ST196 used as reference. Variability results revealed the existence of 30 to 52 single nucleotide polymorphisms (SNPs), resulting in eight sublineages of ST196. The pangenome analysis led to the identification of a novel prophage, pphBhCH20, of the Siphoviridae family in a single isolate of ST196, which suggests that horizontal gene transfer events may drive changes in genomic structure. Conclusions This study contributes to the catalogue of publicly available genomes and provides relevant bioinformatic tools and information for further comparative genomic analyses for B. hyodysenteriae. It reveals that Swiss B. hyodysenteriae isolates of the same ST may have evolved independently over time by point mutations and acquisition of larger genetic elements. In line with this, the third type of mobile genetic element described so far in B. hyodysenteriae, the novel prophage pphBhCH20, has been identified in a single isolate of B. hyodysenteriae of ST196.

show abstract

“…The DNA is then translocated through the tail and the periplasmic tube and is delivered into the cytoplasm. The podovirus ϕ29 infecting B. subtilis possesses a longer tail (about 50 versus 30 nm for T7), which may be related to the thicker CW of Gram-positive bacteria [96]. The virion The podovirus φ29 infecting B. subtilis possesses a longer tail (about 50 versus 30 nm for T7), which may be related to the thicker CW of Gram-positive bacteria [96].…”

Section: Podovirusesmentioning

confidence: 99%

“…The podovirus ϕ29 infecting B. subtilis possesses a longer tail (about 50 versus 30 nm for T7), which may be related to the thicker CW of Gram-positive bacteria [96]. The virion The podovirus φ29 infecting B. subtilis possesses a longer tail (about 50 versus 30 nm for T7), which may be related to the thicker CW of Gram-positive bacteria [96]. The virion structure is similar to that of T7 and P22, except that the tail tube is extended by a distal knob formed by a hexamer of gp9 and probably two copies of gp13 [140].…”

Section: Podovirusesmentioning

confidence: 99%

Phage Adsorption to Gram-Positive Bacteria

Leprince

Mahillon

2023

Viruses

View full text Add to dashboard Cite

The phage life cycle is a multi-stage process initiated by the recognition and attachment of the virus to its bacterial host. This adsorption step depends on the specific interaction between bacterial structures acting as receptors and viral proteins called Receptor Binding Proteins (RBP). The adsorption process is essential as it is the first determinant of phage host range and a sine qua non condition for the subsequent conduct of the life cycle. In phages belonging to the Caudoviricetes class, the capsid is attached to a tail, which is the central player in the adsorption as it comprises the RBP and accessory proteins facilitating phage binding and cell wall penetration prior to genome injection. The nature of the viral proteins involved in host adhesion not only depends on the phage morphology (i.e., myovirus, siphovirus, or podovirus) but also the targeted host. Here, we give an overview of the adsorption process and compile the available information on the type of receptors that can be recognized and the viral proteins taking part in the process, with the primary focus on phages infecting Gram-positive bacteria.

show abstract

Structure and Function of Bacteriophages

Cited by 13 publications

References 181 publications

Thermoresponsive C22 phage stiffness modulates the phage infectivity

Thermoresponsive C22 phage stiffness modulates the phage infectivity

Whole-genome analyses reveal a novel prophage and cgSNPs-derived sublineages of Brachyspira hyodysenteriae ST196

Phage Adsorption to Gram-Positive Bacteria

Contact Info

Product

Resources

About