Structure and Function of Cholinergic Synaptic Vesicles

Stadler, H.; Kiene, Marie-Luise; Harlos, Peter; Welscher, U.

doi:10.1007/978-3-642-70940-1_8

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…These findings demonstrate a striking similarity among vesicles of quite diverse types from three different species. The vesicles we have looked at store transmitters and other materials that differ in size, charge, and other characteristics: the photoreceptor terminals almost certainly release acidic amino acids (reviewed in Miller and Slaughter, 1986); the Torpedo vesicles and the nerve terminals in the adrenal medulla store acetylcholine and nucleotides (e.g., Reichardt and Kelly, 1983;Whittaker, 1984;Stadler et al, 1985); the granules of the adrenal medulla store catecholamines (which are cationic) along with substantial quantities of acidic proteins (e.g., Falkensammer et al, 1985;Winkler and Fischer-Colbrie, 1985); and the terminals in the inner plexiform layer and central nervous system are heterogenous, with different terminals utilizing different transmitters (cf. Marc, 1986).…”

Section: Discusslonmentioning

confidence: 99%

“…It may be germane that many secretory structures, including at least some classes of synaptic vesicles and secretory granules of the adrenal medulla and other glands, maintain a pH gradient across their membranes, such that the vesicle interior is at a markedly lower pH than the cytoplasm outside (e.g., Rudnick et al, 1985;Gainer et al, 1985;Moore et al, 1983;Fuldner and Stadler, 1982;Carty et al, 1982;Shioi and Ueda, 1985); these gradients are thought to be important for the transport of transmitters into the vesicles (e.g., Johnson et al, 1985;Stadler et al, 1985); Schuldiner et al, 1985). Although the gradients are established chiefly through active proton pumping (Al-Awqati, 1986;Rudnick, 19861, anions within the vesicles could help stabilize them or affect the accompanying electrical potentials.…”

Section: Discusslonmentioning

confidence: 99%

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Lead‐dependent deposits in diverse synaptic vesicles: Suggestive evidence for the presence of anionic binding sites

et al. 1987

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We have observed electron dense deposits dependent on incubation of aldehyde-fixed tissues with lead ions within synaptic vesicles of several types of neurons that differ in the neurotransmitters utilized and in the secretory granules of the adrenal medulla. Evidently, vesicle components that can interact with lead ions are widespread. A plausible explanation for the occurrence of the deposits is the presence of anionic binding sites within the vesicles. This would agree well with other biochemical, cytochemical, and immunocytochemical evidence, such as that indicating the presence of sulfated macromolecules in certain synaptic vesicles. Anionic binding sites could play significant roles by participating in processes such as Ca2+ storage, stabilization of pH gradients, or the control of osmotic phenomena.

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Section: Discusslonmentioning

confidence: 99%

Section: Discusslonmentioning

confidence: 99%

Lead‐dependent deposits in diverse synaptic vesicles: Suggestive evidence for the presence of anionic binding sites

et al. 1987

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“…a central role in neurotransmission as the transmitter storing and releasing organelles of the nerve terminal.In recent years progress has been made in functional identification of vesicle specific components.Most of this work has been done on synaptic vesicles isolated from electric organs of Torpedo marmorata(Stadler et al,1985 ) a purely cholinergic model system. More recently a few proteins specific to brain synaptic vesicles have been isolated and characterized as well.…”

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confidence: 99%

Mechanisms Regulating the Expression and Function of Acetylcholine Receptor

Witzemann

Sakmann

1988

Cellular and Molecular Basis of Synaptic Transmission

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Presynaptic effects of peptides at cholinergic synapses. In: Dowdall MJ, Hawthorne IN (eds) Cellular and Molecular Basis of Cholinergic Function. Horwood, Chichester, pp 454-459 De Robertis E, Arnaiz GR de L, de Iraldi AP (1962) Isolation of synaptic vesicles from nerve endings of the rat brain. Nature (Lond) 194:794-795 Dowdall MJ, Zimmermann H (1977) The isolation of pure cholinergic nerve terminI sacs (T-sacs) from the electric organ of juvenile Torpedo. Neuroscience 2:405-421 Ducis I, Whittaker VP (1985) High affinity sodium-gradientdependent transport of choline into vesiculated presynaptic plasma membrane fragments from the electric organ of Torpedo marmorata and reconstitution of the solubilized transporter into liposomes. Biochim biophys Acta 815:109-127 Fiedler W, Borroni E, Ferretti P (1986) An immunohistochemical study of synaptogenesis in the electric organ of Torpedo marmorata by use of antisera to vesicular and presynaptic plasma membrane components. Cell Tiss Res 246:439-446 Giompres PE, Whittaker VP (1986) The density and free water of cholinergic synaptic vesicles as a function of osmotic pressure. Biochim biophys Acta 882:398-409 Giompres PE, Zimmermann H, Whittaker VP (1981a) Purification of small dense vesicles from stimulated Torpedo electric tissue by glass bead column chromatography. Neuroscience 6:765-774 Giompres PE, Zimmermann H, Whittaker VP (1981b) Changes in the biochemical and biophysical parameters of cholinergic synaptic vesicles on transmitter release and during a subsequent period of rest. Neuroscience 6:775-785 Gray EG, Whittaker VP (1960) The isolation of synaptic vesicles from the central nervous system. J Physiol 153:35-37P Gray EG, Whittaker VP (1962) The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J Anat (Lond) 96:79-88 Gray EG, Whittaker VP (1981) This week's citation classic. Current Contents 24:16 Horne RW, Whittaker VP (1962) The use of the negative staining method for the electron-microscopic study of subcellular particles from animal tissues. Z Zellforsch 58:1-16 SPECIALIZATIONS OF SUBSYNAPTIC CYTOPLASMS. COMPARISON OFAXOSPINOUS SYNAPSES AND NEUROMUSCULAR JUNCTIONS

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Die cholinerge Nervenendigung

Zimmermann

1987

Naturwissenschaften

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Structure and Function of Cholinergic Synaptic Vesicles

Cited by 8 publications

References 21 publications

Lead‐dependent deposits in diverse synaptic vesicles: Suggestive evidence for the presence of anionic binding sites

Lead‐dependent deposits in diverse synaptic vesicles: Suggestive evidence for the presence of anionic binding sites

Mechanisms Regulating the Expression and Function of Acetylcholine Receptor

Die cholinerge Nervenendigung

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