Structure and Function of Mitochondria

Fat Utilization in Germinating Douglas Fir Seed

“…The trends seem to follow the genesis of cellular organelles. Since the major components of membrane in cellular organelles are protein and phospholipids (8,13,21), the change of P observed here could be accounted for largely by their ontogenetic development. Similar increases were also indicated during germination of watermelon seed (10), rape seed (16,20), and linseed (20).…”

“…Since the unsaponifiable fraction usually includes pigments, waxes, and sterols which are largely associated with chloroplasts, leaf surfaces, and membranes (8,13,14,21), the small increase of US with germination was expected. A twofold increase of sterols in soybean during germination has also been reported (4).…”

Respiratory Chain of Colorless Algae I. Chlorophyta and Euglenophyta

“…and by low temiiperatutre-differen-ce spectra. Figure 3 (27) high NADH and cytochlrome c oxi(lase activities, and the responises ot these 2 activities to inihibitors are alimiost idlentical to the same properties of mitochonll(ria isolated froil higiher p)laits (7,13,24).…”

Studies on ethylene production by sub-cellular fractions /

“…It is clear that sonically treated mitochondria produced the greatest amount of ethylene. From the works of Zeigler (72) and others (68) it is known that the biochemical properties of mitochondria vary greatly with their state of morphological and structural integrity. In our preparative procedure when the fruit is initially disintegrated by a Waring blendor or similar device, many sub-cellular particles may be fragmented, just as they are in any further mechanical washing and dispersing procedure.…”

“…, while the 105,000g fraction represents the remaining "microsomes".The object behind direct isolation of the 40,210gfraction from the supernatant of 4/000g centrifugation was to prepare a cell free system which might be expected to be more active than either of the consecutively separated 35,OOOg and 40,210g fractions alone.It is obvious that this combined fraction contained mitochondria, and fragments of mitochondria, and endoplasmic reticulum.When, according to the accepted procedure, tissues are initially ground in a sucrose-buffer medium, not only the whole cells but also some sub-cellular particles are likely to be disrupted. In addition to these mechanical effects, osmotic effects must be considered.Zeigler andLinnane(72) and others(68) have shown that the structural integrity (and hence the biochemical activity) of mitochondria is dependent on the osmotic environment. This was borne out in our preparative procedure, when we found that the supernatnat from the centrifugation at 40,210g…”