Structure and genetic variability of golden mussel (Limnoperna fortunei) populations from Brazilian reservoirs

Abstract

The golden mussel, Limnoperna fortunei a highly invasive species in Brazil, has generated productive, economical, and biological impacts. To evaluate genetic structure and variability of L. fortunei populations present in fish farms in the reservoirs of Canoas I (CANFF), Rosana (ROSFF), and Capivara (CAPFF) (Paranapanema River, Paraná, Brazil), eight microsatellite loci were amplified. Five of those eight loci resulted in 38 alleles. The observed heterozygosity (Ho) was lower than the expected heterozygosity (He) in all populations, with a deviation from the Hardy–Weinberg equilibrium (HWE). The average value for the inbreeding coefficient (Fis) was positive and significative for all populations. There was higher genetic variability within populations than among them. The fixation index (Fst) showed a small genetic variability among these populations. The occurrence of gene flow was identified in all populations, along with the lack of a recent bottleneck effect. The clustering analysis yielded K = 2, with genetic similarity between the three populations. The results demonstrate low genetic structure and suggest a founding population with greater genetic variability (ROSFF). Our data point to the possible dispersal of L. fortunei aided by anthropic factors in the upstream direction. It was concluded that the three populations presented a unique genetic pool for Paranapanema River, with occurrence of gene flow.

Population structure of the invasive golden mussel (Limnoperna fortunei) on reservoirs from five Brazilian drainage basins

“…The lack of geographic genetic structure was also noticed by Furlan-Murari and colleagues after sequencing 5 microsatellite loci from 3 golden mussel populations over the Paranapanema river [15]. Zhan and colleagues have sequenced 8 microsatellite loci and the mitochondrial cytochrome c oxidase subunit I (COI) gene from 22 South American populations and found 2 supposedly different genetic clusters [13].…”

“…Zhan and colleagues have compared 22 South American populations and found support for relevant human-mediated transportation [13]. Subsequent studies have strengthened this conclusion, although the number of genetic clusters identified has differed [14,15].…”

Bioaccumulation of Metals and Evaluation of Golden Mussels Encrusted on Different Screens of Net Cages

“…The golden mussel a highly invasive species in Brazil and has caused productive, economical, and biological impacts in reservoirs (Furlan-Murari et al, 2019). Cage fouling increases production costs as they reduce the durability of structures and require frequent cleaning of the screens.…”

Study on the Law of Harmful Gas Release from Limnoperna fortunei (Dunker 1857) during Maintenance Period of Water Tunnel Based on K-Means Outlier Treatment

“…Limnoperna fortunei (dunker 1857) belongs to the Mollusca phylum, bivalve class, and mussel family. The adult mussel is 8-30 mm long, closely connected by byssus, stacked in layers, with a clumpy length of up to 15 cm and a thickness of 3-5 cm [1]. A large number of Limnoperna fortunei invade and deposit in pipes, culverts, tunnels, and other structures of water transmission projects, causing structural blockage and corrosion, resulting in serious biological fouling, affecting the normal operation of water transmission projects [2,3].…”