Structure and hybridization properties of phosphoryl guanidine oligonucleotides under crowding conditions

Kanarskaya, Maria A.; Golyshev, Victor M.; Pyshnyi, D. V.; Lomzov, Alexander A.

doi:10.1016/j.bbrc.2021.09.001

Cited by 1 publication

(1 citation statement)

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“…The repeating negative charges on the nucleic acid backbone inhibit passive transport across cell membranes. Recent endeavors to address this challenge target the phosphodiester backbone directly and introduce uncharged linkages, as in alkyl phosphonate nucleic acids (phNA) and phosphoryl guanidine oligonucleotides (PGOs) ( 49 ) or even positively charged guanidine-bridged nucleic acids (GuNA) ( 102 , 104 ). While these approaches are elegant solutions to a physicochemical problem, they are still limited by larger problems like antisense-binding affinity to RNA targets, cost-efficient availability of starting materials, and ease of synthesis.…”

Section: Synthesis Of Orthogonal Biosystemsmentioning

confidence: 99%

The Expanded Central Dogma: Genome Resynthesis, Orthogonal Biosystems, Synthetic Genetics

Gerecht

Freund

Liu

et al. 2023

Annu. Rev. Biophys.

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Synthetic biology seeks to probe fundamental aspects of biological form and function by construction [i.e., (re)synthesis] rather than deconstruction (analysis). In this sense, biological sciences now follow the lead given by the chemical sciences. Synthesis can complement analytic studies but also allows novel approaches to answering fundamental biological questions and opens up vast opportunities for the exploitation of biological processes to provide solutions for global problems. In this review, we explore aspects of this synthesis paradigm as applied to the chemistry and function of nucleic acids in biological systems and beyond, specifically, in genome resynthesis, synthetic genetics (i.e., the expansion of the genetic alphabet, of the genetic code, and of the chemical make-up of genetic systems), and the elaboration of orthogonal biosystems and components.

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Section: Synthesis Of Orthogonal Biosystemsmentioning

confidence: 99%