Structure and Immunological Characterization of the Capsular Polysaccharide of a Pyrogenic Liver Abscess Caused by Klebsiella pneumoniae

Fu, Yang; Yang, Yu‐Liang; Liao, Pei‐Chun; Chou, Ju-Ching; Tsai, Keng‐Chang; Yang, An-Suei; Sheu, Fuu; Lin, Tzu‐Lung; Hsieh, Pei-Fang; Wang, Jann-Tay; Hua, Kuo-Feng; Wu, Shih‐Hsiung

doi:10.1074/jbc.m111.222091

Cited by 59 publications

(55 citation statements)

References 71 publications

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“…When pretreated with Cur, we observed that Cur effectively attenuated the LPS-induced increase of intracellular ROS and exhibited potent free radical scavenging activity. ROS have been observed to participate in the TLR4-mediated inflammatory signaling pathway [45] . In our study, we inhibited the action of NADPH oxidase, which produces ROS, using DPI.…”

Section: Wwwchinapharcom Meng Z Et Almentioning

confidence: 99%

Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways

et al. 2013

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Aim: To investigate whether curcumin (Cur) suppressed lipopolysaccharide (LPS)-induced inflammation in vascular smooth muscle cells (VSMCs) of rats, and to determine its molecular mechanisms. Methods: Primary rat VSMCs were treated with LPS (1 μg/L) and Cur (5, 10, or 30 μmol/L) for 24 h. The levels of MCP-1, TNF-α, and iNOS were measured using ELISA and real-time RT-PCR. NO level was analyzed with the Griess reaction. Western-blotting was used to detect the activation of TLR4, MAPKs, IκBα, NF-κB p65, and the p47 phox subunit of NADPH oxidase in the cells. Results: Treatment of VSMCs with LPS dramatically increased expression of inflammatory cytokines MCP-1 and TNF-α, expression of TLR4 and iNOS, and NO production. LPS also significantly increased phosphorylation of IκBα, nuclear translocation of NF-κB (p65) and phosphorylation of MAPKs in VSMCs. Furthermore, LPS significantly increased production of intracellular ROS, and decreased expression of p47 phox subunit of NADPH oxidase. Pretreatment with Cur concentration-dependently attenuated all the aberrant changes in LPS-treated VSMCs. The LPS-induced overexpression of MCP-1 and TNF-α, and NO production were attenuated by pretreatment with the ERK inhibitor PD98059, the p38 MAPK inhibitor SB203580, the NF-κB inhibitor PDTC or anti-TLR4 antibody, but not with the JNK inhibitor SP600125. Conclusion: Cur suppresses LPS-induced overexpression of inflammatory mediators in VSMCs in vitro via inhibiting the TLR4-MAPK/ NF-κB pathways, partly due to block of NADPH-mediated intracellular ROS production.

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Section: Wwwchinapharcom Meng Z Et Almentioning

confidence: 99%

Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways

et al. 2013

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“…Because of most the hydrophobic aldoderivatives gives a higher signals than the native glycan in instrumental determination so in recent years, matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) MS with tandem mass instrument has become a powerful tool for the structural determination of the non-derivatived/derivatived glycans. MS is capable of providing structural information for oligosaccharides, although complete structural determination typically required several analytical technologies including tandem mass, GC/MS linkage analysis, exo-/endo-glycosidase digestions, and NMR (Yoo & Yoon 2005;Kim et al, 2006;Kukushkin et al, 2011;Yang et al, 2011). MS methods are used to have molecular weights and fragment ions information producing with tiny sample loading, on the other hand, MS provides a higher sensitivity than other glycan analytic methods.…”

Section: Introductionmentioning

confidence: 99%

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Yang¹

2012

Tandem Mass Spectrometry - Applications and Principles

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“…The major bacterial surface components that are essential for the virulence of K. pneumoniae are capsular polysaccharide (CPS) and lipopolysaccharide (LPS) (4)(5)(6). In a previous study, we demonstrated that CPS of the PLA-causing K. pneumoniae K1 serotype (K1-CPS) induces tumor necrosis factor alpha (TNF-␣) and interleukin-6 (IL-6) secretion by macrophages through the toll-like receptor 4 (TLR4), and that these effects are lost when pyruvation and O-acetylation of K1-CPS are chemically destroyed (7). However, the regulation of the diverse inflammatory responses elicited by K1-CPS is unclear.…”

mentioning

confidence: 99%

Capsular Polysaccharide Is Involved in NLRP3 Inflammasome Activation by Klebsiella pneumoniae Serotype K1

Hua

Chiu

et al. 2015

Infect Immun

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g Klebsiella pneumoniae (strain 43816, K2 serotype) induces interleukin-1␤ (IL-1␤) secretion, but neither the bacterial factor triggering the activation of these inflammasome-dependent responses nor whether they are mediated by NLRP3 or NLRC4 is known. In this study, we identified a capsular polysaccharide (K1-CPS) in K. pneumoniae (NTUH-K2044, K1 serotype), isolated from a primary pyogenic liver abscess (PLA K. pneumoniae), as the Klebsiella factor that induces IL-1␤ secretion in an NLRP3-, ASC-, and caspase-1-dependent manner in macrophages. K1-CPS induced NLRP3 inflammasome activation through reactive oxygen species (ROS) generation, mitogen-activated protein kinase phosphorylation, and NF-B activation. Inhibition of both the mitochondrial membrane permeability transition and mitochondrial ROS generation inhibited K1-CPS-mediated NLRP3 inflammasome activation. Furthermore, IL-1␤ secretion in macrophages infected with PLA K. pneumoniae was shown to depend on NLRP3 but also on NLRC4 and TLR4. In macrophages infected with a K1-CPS deficiency mutant, an lipopolysaccharide (LPS) deficiency mutant, or K1-CPS and LPS double mutants, IL-1␤ secretion levels were lower than those in cells infected with wild-type PLA K. pneumoniae. Our findings indicate that K1-CPS is one of the Klebsiella factors of PLA K. pneumoniae that induce IL-1␤ secretion through the NLRP3 inflammasome.

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Structure and Immunological Characterization of the Capsular Polysaccharide of a Pyrogenic Liver Abscess Caused by Klebsiella pneumoniae

Cited by 59 publications

References 71 publications

Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways

Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Capsular Polysaccharide Is Involved in NLRP3 Inflammasome Activation by Klebsiella pneumoniae Serotype K1

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