Structure and nucleotide sequence of the region encoding the mobilization proteins of plasmid CloDF13

Putten, Arnold J. van; Jochems, G. J.; Lang, Ronald de; Nijkamp, H. J. J.

doi:10.1016/0378-1119(87)90305-2

Cited by 13 publications

(7 citation statements)

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“…CloDF13 is a 9957 bp bacteriocinogenic plasmid isolated from Enterobacter cloacae [95]. Its genetic organization, gene expression and DNA replication have attracted attention during the past three decades [96]. CloDF13 exhibits broad host range for mobilization.…”

Section: The Clodf13 Familymentioning

confidence: 99%

A classification scheme for mobilization regions of bacterial plasmids

et al. 2004

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Transmissible plasmids can be classified according to their mobilization ability, as being conjugative (self-transmissible) or mobilizable (transmissible only in the presence of additional conjugative functions). Naturally occurring mobilizable plasmids carry the genetic information necessary for relaxosome formation and processing, but lack the functions required for mating pair formation. Mobilizable plasmids have a tremendous impact in horizontal gene transfer in nature, including the spread of antibiotic resistance. However, analysis of their promiscuity and diversity has attracted less attention than that of conjugative plasmids. This review will focus on the analysis of the diversity of mobilizable plasmids. For this purpose, we primarily compared the amino acid sequences of their relaxases and, when pertinent, we compared these enzymes with conjugative plasmid relaxases. In this way, we established phylogenetic relationships among the members of each superfamily. We conducted a database and literature analysis that led us to propose a classification system for small mobilizable plasmids in families and superfamilies according to their mobilization regions. This review outlines the genetic organization of each family of mobilization regions, as well as the most relevant properties and relationships among their constituent encoded proteins. In this respect, the present review constitutes a first approach to the characterization of the global gene pool of mobilization regions of small mobilizable plasmids.

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Section: The Clodf13 Familymentioning

confidence: 99%

A classification scheme for mobilization regions of bacterial plasmids

et al. 2004

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“…While CloDFI3 appears to have functionally similar mobilization properties (Van Putten et al 1987), it is notable that its mob protein sequences have no detectable similarity to those of any of the above plasmids. Furthermore, these genes are conserved in two related plasmids ColK and ColA, suggesting that all three have diverged from a common ancestral plasmid capable of being mobilized.…”

Section: Discussionmentioning

confidence: 94%

Characterization of the ColE1 mobilization region and its protein products

1989

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A third of the 6.6 kb genome of ColE1 is devoted to mobilization (mob) genes necessary to promote its specific transfer in the presence of conjugative plasmids. The mob region is genetically complex: two mob genes are entirely overlapped by a third. Oligonucleotide-directed mutagenesis was used to insert an amber codon into one of the overlapped genes and make possible a full complementation analysis of mob. Four mob genes essential for mobilization by R64drd11 were thus identified. Fragments of mob were subcloned under control of the Ptac promoter in a suitable vector, overexpressed in minicells and the mobilization proteins visualized. A comprehensive alignment of the mob region of ColE1 with those of its close relatives ColK and ColA demonstrating that the four essential mob genes are conserved is also presented.

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“…The high content of positively charged residues of Orf2 and the similarity with a protein probably involved in DNA based mechanisms is suggestive of a DNA-binding protein which is probably involved in the transposition mechanism. The predicted protein Orf3 presented sequence similarity of 39.4% with the protein encoded by the mobB gene of Escherichia coli (Van Putten et al, 1987), suggesting the presence of a conjugative mechanism connected with the transposition modalities. A potential application of IS1999 can be the development of a Bifidobacterium mutagenesis system.…”

Section: Discussionmentioning

confidence: 99%

Stability of recombinant plasmids on the continuous culture of Bifidobacterium animalis ATCC 27536

Vara

Rossi

Altomare

et al. 2003

Biotech & Bioengineering

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Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without selective pressure. The rate of plasmid loss (R) and the specific growth rate difference (delta mu) between plasmid-free and plasmid-carrying cells were calculated for each plasmid and their relationship with plasmid size was studied. It was observed that both R and the numerical value of delta mu increased exponentially with plasmid size. The exponential functions correlating the specific growth rate difference and the rate of plasmid loss with the plasmid molecular weight were determined. Furthermore, the smallest of the plasmids studied, pLAV (4.3-kb) was thoroughly characterized by means of its complete nucleotide sequence. It was found that it contained an extra DNA fragment, the first bifidobacterial insertion sequence characterised, named IS 1999.

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Structure and nucleotide sequence of the region encoding the mobilization proteins of plasmid CloDF13

Cited by 13 publications

References 20 publications

A classification scheme for mobilization regions of bacterial plasmids

A classification scheme for mobilization regions of bacterial plasmids

Characterization of the ColE1 mobilization region and its protein products

Stability of recombinant plasmids on the continuous culture of Bifidobacterium animalis ATCC 27536

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