Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis.

Vold, Barbara S.

doi:10.1128/mmbr.49.1.71-80.1985

Cited by 54 publications

(38 citation statements)

References 38 publications

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“…The intergenic spacers of V. parahaemolyticus IFO 12711 are unique, compared to the other species. An intergenic spacer without a tRNA gene has been reported from Bacillus subtilis [15]. E. coli has two di¡erent types of spacer : one contains the tRNA Glu gene, while the other contains the tRNA Ile and tRNA Ala genes [16].…”

Section: Resultsmentioning

confidence: 99%

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Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Maeda¹

2000

FEMS Microbiology Letters

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The 16S^23S rRNA intergenic spacers (IGS) of Vibrio parahaemolyticus were PCR-amplified and cloned with pT7Blue T vector. A total of six clones were isolated dependent on size difference. The clones were different with respect to both the number and the composition of the tRNA genes included, and were designated IGS-0, IGS-E, IGS-IA, IGS-AE, IGS-EKV and IGS-EKAV. IGS-EKAV included the cluster of tRNA Glu -tRNA Lys -tRNA Ala -tRNA Val ; IGS-EKV, tRNA Glu -tRNA Lys -tRNA Val ; IGS-AE, tRNA Ala -tRNA Glu ; IGS-IA, tRNA Ile -tRNA Ala ; and IGS-E, tRNA Glu . IGS-0 had no tRNA gene. Some similarities were found in the nucleotide sequence of the non-coding regions flanked by the tRNA genes. The structure difference found in the spacers is meaningful for elucidating the evolutionary line of each ribosomal RNA operon and the profile is applicable as a molecular marker of the bacterium. ß

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Section: Resultsmentioning

confidence: 99%

“…The variation within the spacer region found in strain IFO 12711 was greater than in B. subtilis and E. coli. B. subtilis has 10 rrn operons, but only two types [15]. E. coli has seven rrn operons, but only two types [16].…”

Section: Resultsmentioning

confidence: 99%

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Maeda¹

2000

FEMS Microbiology Letters

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“…Although many aspects of bacterial gene organization are retained in chloroplast DNA (summarized in Gray, 1986), there are significant differences which might necessitate altered mechanisms of RNA metabolism. For example, bacterial tRNA genes are frequently clustered (Fournier and Ozeki, 1985;Vold, 1985;Fukada and Abelson, 1980) and transcribed into polycistronic precursors (reviewed by King et al, 1986;Deutscher, 1984;Gegenheimer and Apirion, 1981). In plant chloroplasts, just as in eukaryotes, tRNA genes are generally not clustered (Shinozaki et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Novel mechanisms for maturation of chloroplast transfer RNA precursors

1988

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Despite the prokaryotic origins of chloroplasts, a plant chloroplast tRNA precursor is processed in a homologous in vitro system by a pathway distinct from that observed in Escherichia coli, but identical to that utilized for maturation of nuclear pre‐tRNAs. The mature tRNA 5′ terminus is generated by the site‐specific endonucleolytic cleavage of an RNase P (or P‐type) activity. The 3′ end is likewise produced by a single precise endonucleolytic cut at the 3′ terminus of the encoded tRNA domain. This is the first complete structural characterization of an organellar tRNA processing system using a homologous substrate. In contrast to eubacterial RNase P, chloroplast RNase P does not appear to contain an RNA subunit. The chloroplast activity bands with bulk protein at 1.28 g/ml in CsCI density gradients, whereas E.coli RNase P bands as ribonucleoprotein at 1.73 g/ml. Chloroplast RNase P activity survives treatment with micrococcal nuclease (MN) at levels 10‐ to 100‐fold higher than those required to totally inactivate the E.coli enzyme. The chloroplast system is sensitive to a suppression of tRNA processing, caused by binding of inactive MN to pre‐tRNA substrate, which is readily overcome by addition of carrier RNA to the assay.

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“…A hexanucleotide region sharing some identtty with the consensus sequence of stringent response elements from B. subtilis (GT(C/T)G(C/ T)(T/Pu), see [27]) is overlined. In addition.…”

Section: Resultsmentioning

confidence: 99%

“…Further comparison of the Synechocystis 6803 trnQ and trnE genes reveals that the regions immediately downstream of the putative '-10' elements of trnQ and trnE are also somewhat similar. Both regions contain a short A-rich domain, followed by a region with some similarity to the hexanucleotide consensus sequence of the stringent response element of B. subtilis [27]. Possibly these DNA elements mediate a stringent response in Synechocystis 6803 in a manner similar to that observed in B. subtilis, as well as E. coli [28].…”

Section: The Trnq Gene Of Synechocystis 6803mentioning

confidence: 92%

The genes aroA and trnQ are located upstream of psbO in the chromosome of Synechocystis 6803

1993

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We have identified the existence of two genes, trnQ and aroA, located upstream of the psbO gene in Synechocystis sp. PCC 6803. The trnQ gene encodes a glutamine‐specific transfer RNA (tRNAGln) and the sequence given is the first reported for any cyanobacterium. The gene seems to exist as a single copy since its deletion results in nonviable mutation. The aroA gene encodes for 5‐enolpyruvylshikimate 3‐phosphate synthase and its discovery in the genome of Synechocystis 6803 is the first genetic evidence for the existence of the shikimate biosynthetic pathway in cyanobacteria. Interestingly, the partial sequence shares close homologies with the sequences of aroA from Gram‐positive bacteria.

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Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis.

Abstract: Two large clusters of tRNA genes distal to rRNA gene sets may initiate transcription from within the tRNA genes in addition to being part of the larger rRNA transcriptional unit

Cited by 54 publications

References 38 publications

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Structural variation in the 16S–23S rRNA intergenic spacers of Vibrio parahaemolyticus

Novel mechanisms for maturation of chloroplast transfer RNA precursors

The genes aroA and trnQ are located upstream of psbO in the chromosome of Synechocystis 6803

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