Structure and properties of collagen vitrigel membranes for ocular repair and regeneration applications

Calderón‐Colón, Xiomara; Xia, Zhiyong; Breidenich, Jennifer; Mulreany, Daniel G.; Guo, Qiongyu; Uy, O. Manuel; Tiffany, Jason E.; Freund, David; McCally, Russell L.; Schein, Oliver D.; Elisseeff, Jennifer H.; Trexler, Morgana M.

doi:10.1016/j.biomaterials.2012.07.062

Cited by 70 publications

(58 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even after introducing culture medium in the capillary, the 3D membrane structure was self-sustained, and the swelling of the membrane was so small that the whole microchannels were kept opened (Fig.3d). This result shows enhanced mechanical properties of the vitrified collagen compared to conventional collagen hydrogel as previously reported [11,12]. Although vitrified collagen has attracted attention in tissue engineering field, the usage has been limited to 2-D simple structures due to lack of processing methods.…”

Section: -D Scaffold Fabricationsupporting

confidence: 73%

Membrane microchannel made of collagen with self-assembled micro fibril structures for tissue engineering

Ikeuchi

Ikuta

2013

2013 Transducers &Amp; Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems

View full text Add to dashboard Cite

This paper describes a new strategy for regeneration of thick tissues in vitro by inserting artificial capillary network sheets made of vitrified collagen membrane between cell layers. The membrane microchannel sheet was fabricated by drying collagen gel on a template under centrifugal force. After sealing with another membrane, 100m microchannels made of 3m-thick vitrified collagen membrane were successfully fabricated. Since the process did not require chemical cross-linking or heating above denaturation temperature, the fabricated structure keeps natural collagen fibrils. The cyto-compatibility of the structure was confirmed by culturing HUVEC on the membrane.

show abstract

Section: -D Scaffold Fabricationsupporting

confidence: 73%

Membrane microchannel made of collagen with self-assembled micro fibril structures for tissue engineering

Ikeuchi

Ikuta

2013

2013 Transducers &Amp; Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems

View full text Add to dashboard Cite

show abstract

“…To see the effect of centrifugal force on imprinting, a control experiment was carried out by vitrifying the same collagen hydrogel on a template in a static desiccator according to the previously reported protocol [10][11][12]. While the depth of the microchannel fabricated by CIV process reaches down to 50m (Fig.5a), only the surface topology of the template was imprinted on the membrane produced by static desiccation (Fig.5b).…”

Section: -D Scaffold Fabricationmentioning

confidence: 99%

Centrifugal imprinting during vitrification (CIV) of collagen hydrogel for highly biocompatible 3D membrane scaffold

Ikeuchi

Ikuta

2013

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS)

View full text Add to dashboard Cite

This paper reports on a novel micro imprinting process "CIV" to realize 3-D vascular scaffold made of natural biological polymer. The process is performed by gradual vacuum evaporation of hydrogel on a template under centrifugal force ~500G. This process realizes 3D membrane micro/nano structures of extra cellular matrix polymer (e.g. collagen, elastin) with natural self-assembled fibrous structures kept as same as in vivo. The fabricated structure is highly suitable for cell culture scaffold in tissue engineering since no organic solvent or cross-linking reagent is used in CIV process.

show abstract

“…Thus, CVs most closely mimic the biological composition and structure of the cornea, which makes them a clear option for use as scaffold materials in corneal replacements. The CV membrane is of particular interest in our laboratory: previous studies explored the use of vitrigels as functional cell culture substrates, on in vitro reconstruction of layered corneal structure, and on characterizing structure and properties of CVs …”

Section: Introductionmentioning

confidence: 99%

Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes

Majumdar¹,

Guo²,

Garza-Madrid

et al. 2015

J Biomed Mater Res

Self Cite

View full text Add to dashboard Cite

Collagen vitrigel membranes are transparent biomaterials characterized by a densely organized, fibrillar nanostructure that show promise in the treatment of corneal injury and disease. In this study, the influence of different type I collagen sources and processing techniques, including acid-solubilized collagen from bovine dermis (Bov), pepsin-solubilized collagen from human fibroblast cell culture (HuCC), and ficin-solubilized collagen from recombinant human collagen expressed in tobacco leaves (rH), on the properties of the vitrigel membranes was evaluated. Postvitrification carbodiimide crosslinking (CX) was also carried out on the vitrigels from each collagen source, forming crosslinked counterparts BovXL, HuCCXL, and rHXL, respectively. Collagen membrane ultrastructure and biomaterial properties were found to rely heavily on both collagen source and crosslinking. Bov and HuCC samples showed a random fibrillar organization of collagen, whereas rH vitrigels showed remarkable regional fibril alignment. After CX, light transmission was enhanced in all groups. Denaturation temperatures after CX increased in all membranes, of which the highest increase was seen in rH (14.71°C), suggesting improved thermal stability of the collagen fibrils in the membranes. Noncrosslinked rH vitrigels may be reinforced through CX to reach levels of mechanical strength and thermal stability comparable to Bov.

show abstract

Structure and properties of collagen vitrigel membranes for ocular repair and regeneration applications

Cited by 70 publications

References 30 publications

Membrane microchannel made of collagen with self-assembled micro fibril structures for tissue engineering

Membrane microchannel made of collagen with self-assembled micro fibril structures for tissue engineering

Centrifugal imprinting during vitrification (CIV) of collagen hydrogel for highly biocompatible 3D membrane scaffold

Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes

Contact Info

Product

Resources

About