Structure and properties of methacrylate‐endcapped caprolactone networks with modulated water uptake for biomedical applications

Abstract

Methacrylate-endcapped caprolactone (CLMA) networks were synthesized and copolymerized with 2-hydroxyethyl acrylate (HEA) seeking to tailor the hydrophilicity of the system. The resulting structure of the copolymer network is investigated by differential scanning calorimetry, thermogravimetry, and Fourier transform infrared spectroscopy. The dynamic swelling behavior and the equilibrium water sorption is measured and correlated with the microstructure. The experimental results allow one to conclude that the new material is a random copolymer of both components, HEA and CLMA. The effect of cell attachment and proliferation on the new copolymer networks was observed by in vitro culture of human chondrocytes up to 8 days. Enhanced cellular adhesion, similar to that obtained with tissue culture polystyrene (TCPS), was obtained in the hydrophilized systems. The new copolymers are appropriate for the fabrication of scaffolds with controlled porosity for tissue engineering.

Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches

“…Caprolactone 2-(methacryloyloxy) ethyl ester (CLMA), has previously allowed enhanced cellular adhesion and proliferation [17]. CLMA generates scaffolds with controlled porosity for tissue engineering [18] that may favor the integration of stem cells in the damaged tissue that would allow bone regeneration after differentiation.…”

“…Despite good biocompatibility of biomaterials, many synthetic scaffolds lack cell-adhesive properties and need additional modifications to allow cell-surface interactions. Caprolactone 2-(methacryloyloxy) ethyl ester (CLMA), allows enhanced cellular adhesion and proliferation [17]. CLMA generates scaffolds with controlled porosity for tissue engineering [18] that may favor the integration of stem cells in the damaged tissue that after differentiation would allow sites for regeneration in osteoarticular –related pathologies.…”

Biomaterials for tissue engineering and regenerative medicine: Treatment of musculoskeletal injury and disease

“…Several studies have investigated the influence of microenvironment, in the form of hydrophobicity/hydrophilicity, on chondrocyte behavior; largely on two-dimensional (2D) surfaces [28][29][30][31]. Chondrocytes cultured on hydrophilic 2D surfaces [28], or, in one case, encapsulated in 3D poly(ethylene) glycol (PEG)-based hydrogels [32], exhibited greater growth than on hydrophobic surfaces.…”

Synthesis and properties of caprolactone and ethylene glycol copolymers for neural regeneration

“…In a previous work [9,53] we studied the biological response of chondrocytes and goat bone marrow stromal cells on three-dimensional scaffolds based in caprolactone 2-(methacryloyloxy) ethyl ester (CLMA) with tunable hydrophilic properties. The results showed good cell adhesion, proliferation and differentiation.…”

“…Polyesters like poly lactic acid (PLLA), poly glycolic acid (PGA) and their copolymers poly(lactic-co-glycolic acid) (PLGA) were one of the first polymers used because of their biodegradability through hydrolytic mechanisms, availability, and their ease of processing [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. Hadlock et al [25] made a PLLA foam conduit materials seeded with Schwann cells to promote guide regeneration in the peripheral nerve.…”