Structure and stoichiometry of CTAB–DNA complexes

Abstract

We have studied the structure of cetyltrimethylammonium bromide–DNA complexes using small angle x-ray diffraction and elemental analysis. These complexes exhibit a two-dimensional hexagonal phase. The diffraction data have been analyzed using electron density models based on two different structures of these complexes proposed in the literature, which differ in the micelle to DNA stoichiometry. The structure with a 1:2 micelle–DNA stoichiometry is found to be more consistent with the diffraction data. Furthermore, this structure is also supported by the stoichiometry deduced from elemental analysis. Madelung energies of the two structures, calculated from the electrostatic interaction between their cylindrical constituents, give insight into their relative stability.

Whole-genome resequencing analysis of the medicinal plant Gardenia jasminoides

“…Gardenia YP1 is a wild G. jasminoides in Yanping, Fujian Province. Twenty fresh leaves of gardenia FD and 20 fresh leaves of gardenia YP1 were collected at flowering stage, and genomic DNA was extracted from young leaves by cetyltrimethyl ammonium bromide (CTAB) ( Madhukar et al, 2020 ). The concentration and quality of total genomic DNA were determined by NanoDrop 2000 spectrophotometer (Thermo Fisher Science, Waltham, MA, USA), and the DNA library of Illumina /BGI sequencing (350 bp) was constructed.…”