Structure-based identification and functional characterization of an essential lipocalin in the malaria parasitePlasmodium falciparum

Abstract

Proteins of the lipocalin family are known to bind small hydrophobic ligands and are involved in various physiological processes ranging from lipid transport to oxidative stress responses. The genome of the malaria parasite Plasmodium falciparum contains a single protein PF3D7_0925900 with a lipocalin signature. Using crystallography and small-angle X-ray scattering, we show that the protein has a tetrameric structure of typical lipocalin monomers, hence we name it P. falciparum lipocalin (PfLCN), the first lipocalin structurally and functionally characterized in a single-celled eukaryote. We show that PfLCN is expressed in the intraerythrocytic stages of the parasite and localizes to the parasitophorous and food vacuoles. Conditional knockdown of PfLCN impairs parasite development, which can be rescued by treatment with the radical scavenger Trolox or by temporal inhibition of hemoglobin digestion. This suggests a key function of PfLCN in counteracting oxidative stress induced cell damage during multiplication of parasites within red blood cells.Keywords: blood stage / food vacuole / lipocalin / malaria / oxidative stress compound 2. Images and time-lapse movies were acquired on microscopes of the CSSB imaging facility.

PMRT1, aPlasmodiumspecific parasite plasma membrane transporter is essential for asexual and sexual blood stage development

“…GlmS based knockdown assay was adapted from previously published assays (42,76). To induce knockdown 2.5 or 5 mM glucosamine was added to highly synchronous early rings stage parasites.…”