Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin

Dai, Longhai; Li, Hao; Huang, Jian‐Wen; Hu, Yumei; He, Min; Yang, Yu; Min, Jian; Guo, Rey‐Ting; Chen, Chun‐Chi

doi:10.1016/j.ijbiomac.2022.09.121

International Journal of Biological Macromolecules

2022

DOI: 10.1016/j.ijbiomac.2022.09.121

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Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin

Longhai Dai

Hao Li

Jian‐Wen Huang

et al.

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2024

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Cited by 9 publications

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Metarhizium acridum transcription factor MaFTF1 negatively regulates virulence of the entomopathogenic fungus by controlling cuticle penetration of locusts

Hong,

Yang,

Wang

et al. 2024

Pest Management Science

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BACKGROUNDThe entomopathogenic fungus (EPF) Metarhizium acridum, a typical filamentous fungus, has been utilized for the biological control of migratory locusts (Locusta migratoria manilensis). Fungal‐specific transcription factors (TFs) play a crucial role in governing various cellular processes in fungi, although TFs with only the Fungal_trans domain remain poorly understood.RESULTSIn this study, we identified a unique fungal‐specific TF in M. acridum, named MaFTF1, which contains only a Fungal_trans domain and functions as a negative regulator of M. acridum virulence by influencing cuticle penetration. The virulence of the MaFTF1 knockout strain (ΔMaFTF1) against L. migratoria was increased, with a median lethal time (LT50) ~0.91 days shorter than that of the wild‐type (WT) strain when inoculated topically, mimicking natural infection conditions. Correspondingly, ΔMaFTF1 penetrated the cuticle earlier than did the WT strain. Our investigation revealed that the development of appressoria was accelerated in ΔMaFTF1 compared with the WT strain. Furthermore, the appressoria of the ΔMaFTF1 displayed higher turgor pressure and an upregulated expression of fungal hydrolases active toward the insect cuticle. RNA sequencing analysis indicated that the differences in appressorium behavior between the strains were due to MaFTF1 regulating a complex metabolism pathway.CONCLUSIONThis study revealed that MaFTF1 acts as a negative regulator of virulence, impacting the process of cuticle penetration by slowing the formation of appressoria, decreasing their turgor pressure, and reducing the expression of hydrolases in appressoria, revealing an unexpected strategy in the EPFs. © 2024 Society of Chemical Industry.

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Metarhizium acridum transcription factor MaFTF1 negatively regulates virulence of the entomopathogenic fungus by controlling cuticle penetration of locusts

Hong,

Yang,

Wang

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

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Identification and application of a novel patulin degrading enzyme from Cyberlindnera fabianii

Song,

Xu,

Guang

et al. 2024

Food Research International

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Structural and functional insights into the self-sufficient flavin-dependent halogenase

Dai,

Li,

Dai

et al. 2024

International Journal of Biological Macromolecules

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Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin

Cited by 9 publications

References 43 publications

Metarhizium acridum transcription factor MaFTF1 negatively regulates virulence of the entomopathogenic fungus by controlling cuticle penetration of locusts

Metarhizium acridum transcription factor MaFTF1 negatively regulates virulence of the entomopathogenic fungus by controlling cuticle penetration of locusts

Identification and application of a novel patulin degrading enzyme from Cyberlindnera fabianii

Structural and functional insights into the self-sufficient flavin-dependent halogenase

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