Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (<i>Haliotis fulgens</i>) and yellow (<i>H. corrugata</i>) abalone from Baja California Sur, Mexico

Cicala, Francesco; Cisterna-Céliz, José Alejandro; Moore, James Douglas; Rocha‐Olivares, Axayácatl

doi:10.7287/peerj.preprints.3233

Cited by 3 publications

(11 citation statements)

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“…As we previously reported [33], Tenericutes was by far the predominant bacterial phylum in both morphologically healthy and WS-stressed abalone of both species. Moreover, both HF and HC possessed similar community structures at the highest taxonomic resolution.…”

Section: Microbiota Structural Compositionsupporting

confidence: 76%

“…This may be particularly important in abalone, as higher interspeci c microbiota diversity is generally explained by an increase in Alphaand Gammaproteobacteria [6,33,59], both of which represent the main taxonomic groups that produce antimicrobial compounds in abalone species [60,61]. Thus, we posit that the lower susceptibility to WS observed in HC may be explained by both a high prevalence of Alphaand Gammaproteobacteria [33] and the production of associated byproducts. Nevertheless, additional efforts are needed to validate this hypothesis.…”

Section: Discussionmentioning

confidence: 91%

“…Indeed, in a redundant system, the removal of species may not necessary result in a loss of functionality of the entire system, as other taxonomic groups may carry out the ecological tasks of the species that are removed [27]. This was particular evident in HC, where a higher number of bacterial OTU species were found to enrich the same KEGG pathway [33]; thus, reduced bacterial diversity does not necessarily result in a functional misregulation of the entire microbiota. On the other hand, from a functional prospective, the increase in α-diversity observed in WS-stressed HF should be considered to be stochastic, as predicted by the AKP.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the hypothesis that species-speci c microbiota arrangements among abalone species may lead to different abalone responses to WS remains unexplored. Recently, we compared and reported the structural arrangements and functional capability pro les for the GI microbiotas of healthy HC and HF [33]. The microbiotas of HF appears to be less complex compared to that of HC, as it is mainly dominated by Mycoplasma spp., whereas the microbiotas of HC appears to be composed by a higher number of bacterial operational taxonomic units (OTU) species [33].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we compared and reported the structural arrangements and functional capability pro les for the GI microbiotas of healthy HC and HF [33]. The microbiotas of HF appears to be less complex compared to that of HC, as it is mainly dominated by Mycoplasma spp., whereas the microbiotas of HC appears to be composed by a higher number of bacterial operational taxonomic units (OTU) species [33]. Here, we posited that these microbiota characteristics may lead to microbiota-speci c responses in WS-stressed abalone and that dysbiotic microbiotas would be characterized by a high number of new stochastic bacterial con gurations, as predicted by the aforementioned ecological theories.…”

Section: Introductionmentioning

confidence: 99%

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The role of diversity in mediating microbiota structural and functional responses in two sympatric species of abalone under stressed withering syndrome conditions

Cicala¹,

Cisterna-Céliz²,

Paolinelli³

et al. 2021

Preprint

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Background: The withering syndrome (WS) is an infectious disease initially affecting the gastro-intestinal tract (GI) of wild abalone populations of the coasts of Baja California. In spite of its high incidence, structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. Moreover, it is equally uncertain if interspecific microbiota features, such as the presence or absence of certain bacterial species, their abundances, and their functional capabilities, may prevent or at least lead to different microbiota responses. Healthy Haliotis fulgens and Haliotis corrugata from Baja California Sur (Mexico) harbor species-specific structural and functional microbiota profiles; hence, we hypothesize a distinctive microbiota response under WS-stressed conditions. Here, we compared both the structural arrangements and functional capabilities of healthy and dysbiotic microbiotas using 454 pyrosequencing high throughput sequencing technologies and PICRUSt v.2 outputs, respectively.Results: Our findings suggest that the extent to which WS may involve structural and functional changes in GI microbiotas is contingent on the microbiota diversity itself. Indeed, we report significant structual alterations in the less complex microbiotas of H. fulgens, which in turn led to a significant downregulation of several metabolic activities conducted by GI bacteria. Conversely, the effects of WS were marginal in more complex bacterial communities, as in H. corrugata, in which no significant structural and functional changes were detected. Conclusions: Our results provide new insights concerning the role of microbiome diversity in abalone health and the etiology of WS. Notably, complex bacterial communities appear to be less affected by WS than less complex microbiotas. Moreover, our insights suggest that structural changes observed under WS-stressed conditions may be considered stochastic, as predicted by the Anna Karenina principle, and result in the downregulation of several ecological functions conducted by GI bacteria. Overall, our results support the hypothesis that the occurrence of WS may be associated with shifts in GI microbiotas. Moreover, we propose that the susceptibility to WS that has been reported among abalone species may reflect the natural degree of complexity of the GI microbiomes harbored by each species.

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Section: Microbiota Structural Compositionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The role of diversity in mediating microbiota structural and functional responses in two sympatric species of abalone under stressed withering syndrome conditions

Cicala¹,

Cisterna-Céliz²,

Paolinelli³

et al. 2021

Preprint

Self Cite

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Effects of dietary protein levels on growth performance, serum indexes, PI3K/AKT/mTOR/S6K signalling and intestinal microbiota of abalone Haliotis discus hannai

Sun

et al. 2021

Aquacult Nutr

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A 120‐day feeding trial was conducted to assess the effect of dietary protein levels on growth performance, serum indexes, PI3 K/AKT/mTOR/S6 K signalling pathway and intestinal microbiota of abalone Haliotis discus hannai. Abalones (initial body weight: 15.88–16.54 g; initial shell length 41.70–42.40 mm) were fed nine isoenergetic (~16.08 kJ/g) and isolipidic (~42.0 g/kg) experimental diets with 93.5, 116.9, 151.1, 191.8, 230.3, 275.9, 320.3, 360.5 and 405.9 g/kg protein contents, respectively. Results showed that the optimal dietary protein level for H. discus hannai was determined as 259.4 g/kg through second‐order polynomial regression analysis for weight gain rate (WGR). Serum TP and urea nitrogen levels were significantly increased when dietary protein levels were higher than 320.3 g/kg (p < .05). The highest gene expressions of phosphatidylinositol 3 kinase (PI3 K), target of rapamycin (mTOR) and ribosomal protein S6 kinase (S6 K) in muscle were found in the treatment with 275.9 g/kg of dietary protein. The excessive dietary protein level (405.9 g/kg) significantly decreased the gene expression of S6 K. Meanwhile, abalone fed with 275.9 g/kg of dietary protein exhibited the highest microbial diversity in intestine as measured by Chao richness and Shannon diversity index. In conclusion, dietary protein level reduced from 259.4 g/kg to 154.0 g/kg or increased from 259.4 to 323.0 g/kg, the WGR of abalone might be depressed with 5% likelihood. Over high (>360.5 g/kg) or low (<151.1 g/kg) dietary protein levels had negative effects on growth performance, serum indexes, PI3 K/AKT/mTOR/S6 K signalling and microbial diversity in intestine of abalone.

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Gastrointestinal tract microbial community of Babylonia areolata and its diversity are closely correlated with the outbreak of disease

Zhao

Wang

et al. 2021

Aquaculture Research

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The intestinal microbiome is important for the health of aquatic animals. However, in Babylonia, the combination of gastrointestinal tract (GI) bacterial symbionts has barely been explored. In this study, from a farm with naturally occurring diseases, we collected Babylonia areolata in three different states, that is “diseased” (group D), “health” (group H) and “subhealth” (group SH) and aimed to reveal the relationship between disease occurrence and GI microbiota of B. areolata. Diversity analysis illustrated that GI microbial diversity of group D was obviously lower than that of group H on phylum level and genus level, and GI microbial diversity of group SH was between group D and H. KEGG analysis showed that the physiological activities of pathogenic microorganisms in group D increased significantly, compared with group H. Metagenomics analysis demonstrated the expressions of virulence genes in group D enhanced obviously, compared with group H. In addition, the diversity of culturable microorganisms from the GI contents of group D was dramatically lower than that of group H. A total of 22 species were obtained from the GI contents of group H. However, only four species were obtained from group D, and two important pathogens, Vibrio harveyi and Vibrio tubiashii, were dominant microbiota. These results indicate that the decline of GI microbial diversity in B. areolata is highly associated with the occurrence of disease. We speculated environmental factors led to the decline of GI microbial diversity, which could promote propagation of pathogenic microbes (such as V. harveyi and V. tubiashii). Our findings provide new insights into the disease outbreak of B. areolata and provide propose that the preservation of GI microbial diversity at the early phases can effectively prevent the occurrence of disease.

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Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (Haliotis fulgens) and yellow (H. corrugata) abalone from Baja California Sur, Mexico

Cited by 3 publications

References 0 publications

The role of diversity in mediating microbiota structural and functional responses in two sympatric species of abalone under stressed withering syndrome conditions

The role of diversity in mediating microbiota structural and functional responses in two sympatric species of abalone under stressed withering syndrome conditions

Effects of dietary protein levels on growth performance, serum indexes, PI3K/AKT/mTOR/S6K signalling and intestinal microbiota of abalone Haliotis discus hannai

Gastrointestinal tract microbial community of Babylonia areolata and its diversity are closely correlated with the outbreak of disease

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