Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

“…Residue C was signed as galacto configuration (Gheysen et al, 2008) but for residue D, most of the protons and carbons had similar distribution with residue C. Interestingly, the chemical shift of O-CH 3 (δ 3.46/58.90) was assigned from the HSQC spectrum, and 1 H resonances for O-CH 3 correlated with C-3 (δ 3.46/81.59) of residue D in the HMBC spectrum showed that O-CH 3 was located on residue D, and C-3 was assigned to H-4/C-3 (δ 4.3/81.59) in HMBC spectrum (Figure 5A). Therefore, residue C was ascribed to →6)-α-D-Galp-(1→, and residue D was assigned to →6)-3-O-Me-α-D-Galp-(1→ (Zhang et al, 2013;Oliveira et al, 2019). The aforementioned results confirmed the existence of 3-O-Me-galactose and further confirmed the results of monosaccharide composition and methylation analysis.…”

“…For residue E, the anomeric signals were found at δ 4.80/ 104.49, which showed the presence of β-link in residue E. The signals from H-1/C-1 to H-6/C-6 were identified COSY and HSQC spectrum (Table 2). The β-configuration of residue E was also inferred by the H-5 and C-5 chemical shifts at δ 3.39 and 79.09 ppm (compared published data δ 3.82/73.34 or δ 3.38/77.00 for β-mannopyranose), respectively (Jansson et al, 1989;Oliveira et al, 2019;Ellefsen et al, 2021).…”

Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota)

“…Analysis of 13 C NMR corroborates the presence of β-glucans as the main signals observed in the spectra of Gi -MRSW and Gi -FSME fractions that are characteristic of this class of compounds [ 31 ]. Signals from the Gi -FPME fraction correspond to a heterogalactan, commonly found in macrofungi, which contains a main chain of α-Gal p (1→6)-linked, partially substituted in O-2 by non-reducing ends of β-Man p and α-Fuc p [ 32 ]. The signals observed in the anomeric region of the 13 C NMR spectrum of the Gi -FPME fraction ( Figure 2 ) refer to the C-1 of the non-reducing ends of β-Man p (δ 104.36) and α-Fuc p (δ 104.14), and the main chain units consisting of α-Gal p 6- O - (δ 100.89) and 2,6-di- O -substituted (δ 101.48 and 101.18).…”

“…The signals observed in the anomeric region of the 13 C NMR spectrum of the Gi -FPME fraction ( Figure 2 ) refer to the C-1 of the non-reducing ends of β-Man p (δ 104.36) and α-Fuc p (δ 104.14), and the main chain units consisting of α-Gal p 6- O - (δ 100.89) and 2,6-di- O -substituted (δ 101.48 and 101.18). The glycosidic linkage of type 1→6 of the α-Gal p units of the main chain was confirmed by the signal in δ 69.85, while the substitutions in O-2 of these units by non-reducing terminals of α-Fuc p and β-Man p were confirmed by the signs in δ 80.54 and 79.82, respectively [ 32 ]. Although the 13 C NMR spectra of Gi -MRSW ( Figure 2 ) and Gi -FSME ( Figure 2 ) fractions suggest the majority presence of β-glucans in these fractions, they have distinct structures.…”

Data_Sheet_1.xlsx

“…Therefore, thermotolerance is important for H. marmoreus during both vegetative growth and fruiting-body formation. Previous studies on H. marmoreus have mainly focused on cultivation, nutrition and bioactive compounds extracted (Mleczek et al, 2018;Saito et al, 2018;Oliveira et al, 2019;Tsai et al, 2019), but little is known about thermotolerance of this mushroom.…”