Structure elucidation of β-sitosterol with antibacterial activity from the root bark of Malva parviflora

Abstract

The powder of root bark of Malva parviflora (Malvaceae) was successively extracted with petroleum ether (b.p. 60-80 °C), chloroform and ethanol. The chloroform extract showed antibacterial activity against Staphylococcus aureus and Escherichia coli, whereas the ethanolic extract showed antibacterial activity against only S. aureus. The chloroform extract, after column chromatographic separation on silica gel using petroleum ether:chloroform (3:1) as eluent, furnished 98 mg of white crystalline compound. The yield of the compound is 0.316 % (w/w). The compound has a melting point of 134-136 °C and the R f value 0.56 in benzene:chloroform:acetone (1:15:1) on silica gel TLC. The compound was characterized as β-sitosterol by physical properties, chemical test, spectral analysis (FTIR, NMR and MS) and comparing the data obtained from the literature.

α-Glucosidase Inhibition and Molecular Docking Studies of Natural Brominated Metabolites from Marine Macro Brown Alga Dictyopteris hoytii

“…On the basis of above discussion and combined NMR and mass data, the structure of compound 1 was established to be 2-bromodimethylbenzene-1,4-dioate. To the best of our knowledge, both compounds 1 and 2 have not been described earlier as natural products, while compound 2 has been synthesized previously by many researchers [31][32][33] The structures of the known compounds ( Figure 1) were determined as fucosterol (3) [34][35][36], nhexadecanoic acid, methyl ester (4) [37,38], ꞵ-sitosterol (5) [35,39], cerotic acid (6) [40,41], n-octacos-9enoic acid (7) [42,43], and 11-eicosenoic acid (8) [44,45], on the basis of their spectral data and the comparison of their spectral data with those reported in literature. Compound 2 was obtained as a colorless amorphous powder with the molecular formula C 12 H 13 BrO 4 , deduced from the HR-ESI-MS and 13 C-NMR data ( Figure 1).…”

Screening of phytochemicals and toxicity of medicinal plants,Dilleniaspecies, reveals potential natural product resources

“…Some important others such as β‐sitosterol and stigmasterol were lower than 10%. β‐Sitosterol has been shown to have antioxidant and anti‐inflammatory properties (Dighe, Kuchekar, & Wankhede, ; Manral et al, ; Ododo et al, ), and it is considered to play a role in the reduction of cholesterol. Antitumorigenic, antimetastatic, and significant anti‐inflammatory effects have been revealed as a result of application in in vitro studies (Dighe et al, ; Kraft, Freeling, Wang, & Ho, ; Rashed & Fouche, ).…”

“…Meanwhile, only extracts of D. suffruticosa exhibited significant DNA damage in leukocytes ( Figure 4) as shown by a p value < .05 (Table 3). (Dighe, Kuchekar, & Wankhede, 2016;Manral et al, 2016;Ododo et al, 2016), and it is considered to play a role in the reduction of cholesterol. Antitumorigenic, antimetastatic, and significant anti-inflammatory effects have been revealed as a result of application in in vitro studies (Dighe et al, 2016;Kraft, Freeling, Wang, & Ho, 2014;Rashed & Fouche, 2013).…”

“…The plant species of the genus Dillenia , Dilleniaceae family have been used in tribal and folk communities of East Asia for the treatment of multiple ailments and diseases (Alam et al, ; Dubey, Sikarwar, Khanna, & Tiwari, ). Elephant apple ( D. indica ) is a prominent species which contains chemical substances that can be applied for medicinal purposes, such as β‐sitosterol and stigmasterol for their antimicrobial activity (Dey, Mukherjee, De, & Pandey, ; Ododo, Choudhury, & Dekebo, ), as well as vitamin E for its antioxidant features (Muhit, Tareq, Apu, Basak, & Lslam, ; Yeshwante et al, ). These four components also have anti‐inflammatory (Parvin et al, ) and antidiarrheal properties (Clementino‐Neto et al, ; Singh & Gupta, ).…”

Antibacterial and antibiotic potentiating activities of tropical marine sponge extracts

“…Song et al (2010) Of note, at present no other study regarding the antibiotic modulatory effect of marine sponges has been reported, as well as, additional studies aimed at identifying the molecular mechanism underlying the antimicrobial property of this sponge extract remain to be carried out. isolated (Ododo et al 2016, Subramaniam et al 2014, Sanches et al 2005. Sponges of the genus Neopetrosia are still yielding novel bioactive molecules; neopetrocyclamines A and B (Liang et al, 2015), 9'-epi-3β, 3'β-dimethylxestospongin C (Li et al, 2011) and exiguamine A (Brastianos et al, 2006) and this was the impetus for the investigation of a member of this genus.…”