Structure-function assessment of 3D-printed porous scaffolds by a low-cost/open source fused filament fabrication printer

Baier, Raúl Vallejos; Raggio, José I. Contreras; Arancibia, Carlos Toro; Bustamante, Miguel; Perez, Laetitia; Burda, Iurii; Aiyangar, Ameet; Vivanco, Juan

doi:10.1016/j.msec.2021.111945

Cited by 5 publications

(8 citation statements)

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“…Therefore, to determine how gelatin coating and the presence of GO nanoparticles affected the biological behavior of a PCL matrix, the effects of PCL/Gt, PCL/GO, and PCL/2 wt% GO/Gt on cell viability were studied in terms of the cell adhesion and proliferation of hGMSC and were compared with those of neat PCL. As in previous studies, the cells loaded directly onto the plate were used as controls [ 39 , 40 ]. Due to the similarity of the results in forming hydroxyapatite spheres in the bioactivity test, PCL/2 wt% GO was selected for the biological studies.…”

Section: Resultsmentioning

confidence: 99%

“…Human gingival mesenchymal stem cells (hGMSCs) were used to test the biological performance of the scaffolds [ 37 , 38 ]; they were acquired and cultured following our previously reported methods [ 39 , 40 ]. hGMSCs from primary cell cultures were obtained from human participants after approval by the Institutional Review Board of Universidad Adolfo Ibáñez, IRB-UAI, and the Bioethical Research Committee (approval number 54/2019), as reviewed in Benjumeda et al 2020 [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…Electrospun scaffolds were placed in specialized holders, which were manufactured in polylactic acid in an in-house 3D printer [ 39 ]. The holders were sterilized in EtOH 70% overnight, and the scaffolds were sterilized under UV light for at least one hour per side [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cell adhesion efficiency was evaluated as previously reported [ 39 , 40 ]. Briefly, 4.5 × 10 4 hGMSCs in 200 µL of culture media were added on top of each scaffold and placed in each holder for two hours (together with the controls).…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, 200 µL of the supernatant was measured in a plate reader (Biotek 800 TS, Merck (Darmstadt, Germany) at 490 nm. Cell adhesion efficiency is expressed in relation to the control (cells in the well), which was considered to be 100% [ 39 , 42 ].…”

Section: Methodsmentioning

confidence: 99%

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Effect of Gelatin Coating and GO Incorporation on the Properties and Degradability of Electrospun PCL Scaffolds for Bone Tissue Regeneration

Loyo,

Cordoba,

Palza

et al. 2023

Polymers

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Polymer-based nanocomposites such as polycaprolactone/graphene oxide (PCL/GO) have emerged as alternatives for bone tissue engineering (BTE) applications. The objective of this research was to investigate the impact of a gelatin (Gt) coating on the degradability and different properties of PCL nanofibrous scaffolds fabricated by an electrospinning technique with 1 and 2 wt% GO. Uniform PCL/GO fibers were obtained with a beadless structure and rough surface. PCL/GO scaffolds exhibited an increase in their crystallization temperature (Tc), attributed to GO, which acted as a nucleation agent. Young’s modulus increased by 32 and 63% for the incorporation of 1 and 2 wt% GO, respectively, in comparison with neat PCL. A homogeneous Gt coating was further applied to these fibers, with incorporations as high as 24.7 wt%. The introduction of the Gt coating improved the hydrophilicity and degradability of the scaffolds. Bioactivity analysis revealed that the hydroxyapatite crystals were deposited on the Gt-coated scaffolds, which made them different from their uncoated counterparts. Our results showed the synergic effect of Gt and GO in enhancing the multifunctionality of the PCL, in particular the degradability rate, bioactivity, and cell adhesion and proliferation of hGMSC cells, making it an interesting biomaterial for BTE.

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Section: Resultsmentioning

confidence: 99%