Structure–function characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana

Mazorra-Manzano, Miguel A.; Tanaka, Takuji; Dee, Derek R.; Yada, Rickey Y.

doi:10.1016/j.phytochem.2009.12.005

Cited by 22 publications

(12 citation statements)

References 60 publications

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“…and stable between pH 2 and pH 10 was characterized in V. radiata [37]. Similar pattern was described for a recombinant AP in Arabidopsis [38]. In A. colubrina there is also indicated a fourth endopeptidase (E0/pH7), whose class was not determined in this study.…”

Section: Peptidases Detected With Abz-aiaffsrq-eddnp Hydrolysissupporting

confidence: 75%

Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination

Barduche¹,

Livramento²,

Júdice³

et al. 2018

AJPS

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Proteolysis of seed storage proteins (SSP) during germination provides a steady supply of amino acids to the embryo development into seedling. This process is coordinated by different peptidases that act sequentially and overlaid mode. These enzymes are an ancient group evolved separately in a wide structural and functional diversity and have many applications in medicine, pharmacy and industry. However, the knowledge about seed peptidases during germination was obtained from studies almost restricted to the cultivated species. This restriction implies caution about generalizations made from these studies, as well limits the biological knowledge about plant kingdom and technological use from plant peptidases. In this work, a scan of the proteolytic activity was held in germinating seeds of a leguminous subtropical woody tree. Eleven proteolytic activities were detected in protein extracts from embryonic axis and cotyledons. The presence and intensity of these activities varied over time and between these tissues. There was indication that aspartyl-endopeptidases (phytepsins) and cysteine-carboxypeptidases (plant cathepsins) were involved in A. colubrina SSP hydrolysis. These peptidases differ to that commonly involved in germination of the cultivated leguminous. In addition, one of detected phytepsins showed stability on pH scale, which is important for industrial uses. There was also detected a metallo-carboxypeptidase activity, which has been not described in plants. These peptidases must be isolated to confirm or not these indications. However, these data indicate the biological and technological importance of extending the studies about plant peptidases on a diverse genetic basis.How to cite this paper: Barduche, D., do

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Section: Peptidases Detected With Abz-aiaffsrq-eddnp Hydrolysissupporting

confidence: 75%

Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination

Barduche¹,

Livramento²,

Júdice³

et al. 2018

AJPS

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“…This reduced the list to 703 unigenes, a manageable number for a comprehensive study; of these, 75 were chosen as possibly involved in plant response to PPV (Table S3). The list included a wide range of genes coding for proteins as diverse as histones [80], aspartic proteases [81] or polyphenol oxidases [82] and define a clear starting point for future research on plant defense.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection

et al. 2014

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Plum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named ‘Jojo’, develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. Analysis of viral reads from the infected samples allowed reconstruction of a PPV-D consensus sequence. De novo reconstruction showed a second viral isolate of the PPV-Rec strain. RNA-seq analysis of PPV-infected ‘Jojo’ trees identified 2,234 and 786 unigenes that were significantly up- or downregulated, respectively (false discovery rate; FDR≤0.01). Expression of genes associated with defense was generally enhanced, while expression of those related to photosynthesis was repressed. Of the total of 3,020 differentially expressed unigenes, 154 were characterized as potential resistance genes, 10 of which were included in the NBS-LRR type. Given their possible role in plant defense, we selected 75 additional unigenes as candidates for further study. The combination of next-generation sequencing and a Prunus variety that develops a hypersensitive response to PPV infection provided an opportunity to study the factors involved in this plant defense mechanism. Transcriptomic analysis presented an overview of the changes that occur during PPV infection as a whole, and identified candidates suitable for further functional characterization.

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“…Los EFN mostraron actividad a diferentes valores de pH. La actividad detectada a pH ácido (pH 3,5) podría reflejar la actividad de proteasas tipo aspárticas (Dunn, 2002;Mazorra-Manzano et al, 2010) mientras que la actividad en la región neutra y alcalina evidencian la posible presencia de proteasas tipo serina y cisteína, ya que la mayoría de estas enzimas muestran actividad en un amplio rango de pH (Gaur y Wadhwa, 2008;Yadav et al, 2012). Lo anterior indica que los extractos crudos de flor de naranjo podrían ser utilizados para la modificación enzimática de diversos sustratos proteicos en procesos como la producción de hidrolizados proteicos.…”

Section: Resultados Y Discusiónunclassified

HIDROLISIS DE PROTEÍNAS POR UN EXTRACTO ENZIMÁTICO DE FLOR DE NARANJO AGRIO (Citrus aurantium) Y SU POTENCIAL COAGULANTE PARA PRODUCCIÓN DE QUESOS

Mazorra-Manzano

Moreno-Hernández

Torres-Llanez

et al. 2013

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Structure–function characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana

Cited by 22 publications

References 60 publications

Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination

Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination

Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection

HIDROLISIS DE PROTEÍNAS POR UN EXTRACTO ENZIMÁTICO DE FLOR DE NARANJO AGRIO (Citrus aurantium) Y SU POTENCIAL COAGULANTE PARA PRODUCCIÓN DE QUESOS

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Structure–function characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana

Cited by 22 publications

References 60 publications

Proteolysis in the Subtropical Woody Tree &lt;i&gt;Anadenanthera colubrina&lt;/i&gt; (Angico) Seeds during and after Germination

Proteolysis in the Subtropical Woody Tree &lt;i&gt;Anadenanthera colubrina&lt;/i&gt; (Angico) Seeds during and after Germination

Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection

HIDROLISIS DE PROTEÍNAS POR UN EXTRACTO ENZIMÁTICO DE FLOR DE NARANJO AGRIO (Citrus aurantium) Y SU POTENCIAL COAGULANTE PARA PRODUCCIÓN DE QUESOS

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Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination

Proteolysis in the Subtropical Woody Tree <i>Anadenanthera colubrina</i> (Angico) Seeds during and after Germination