Structure identification of soybean peptides and their immunomodulatory activity

Wen, Li; Jiang, Yueming; Zhou, Xuesong; Bi, Huimin; Yang, Bao

doi:10.1016/j.foodchem.2021.129970

Cited by 44 publications

(37 citation statements)

References 38 publications

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“…The amino acid composition of FM was determined in accordance with the method of Wen et al (2021), with modifications. One milliliter of each sample was mixed with 1 mL of 5% sulfosalicylic acid, placed at room temperature for 15 min, and then centrifuged at 5,500 × g for 15 min at 4°C.…”

Section: Amino Acid Analysismentioning

confidence: 99%

Effects of ultra-high pressure treatment on angiotensin-converting enzyme (ACE) inhibitory activity, antioxidant activity, and physicochemical properties of milk fermented with Lactobacillus delbrueckii QS306

Zhao

Wang

et al. 2022

Journal of Dairy Science

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The present study investigated the influence of ultrahigh pressure (UHP) treatment on angiotensin-converting enzyme inhibitory (ACEI) activity and quality of milk fermented with Lactobacillus delbrueckii QS306 after storage. By varying treatment pressure, duration of pressure treatment, and duration of fermentation, optimal process parameters for the UHP treatment of milk fermented with QS306 to enhance ACEI activity were found to be 400 MPa, 10 min, and 48 h, respectively. The degree of ACE inhibition of the fermented milk was 91.63 ± 0.02%. After UHP treatment, ACEI activity, apparent viscosity, concentrations of polypeptides and volatile aromatic substances, umami, and richness had increased significantly, bitterness and astringency were significantly reduced, and antioxidant properties were maintained. In addition, UHP fermented milk maintained a high level of ACEI activity and good quality during storage. Thus, the data represent a valuable reference for improving the storage quality of fermented milk and research for the future development of dairy products with high ACEI activity.

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Section: Amino Acid Analysismentioning

confidence: 99%

Effects of ultra-high pressure treatment on angiotensin-converting enzyme (ACE) inhibitory activity, antioxidant activity, and physicochemical properties of milk fermented with Lactobacillus delbrueckii QS306

Zhao

Wang

et al. 2022

Journal of Dairy Science

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“…Over the past two decades, bioactive food protein hydrolysates and peptides have gained great research interest due to their physiological functions, such as antioxidant ( 4 , 5 ), antibacterial ( 5 ), anticancer ( 6 ), antidiabetic ( 7 ), and antihypertensive properties ( 4 , 8 , 9 ). Besides, they play an important role in immune regulation ( 10 ). β-Conglycinin (βCG, 7S) and glycinin (11S) are the two major proteins of soybean which account for up to 90% of the total protein content.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Antioxidant Properties of Germinated Soybean Protein Hydrolysates

Zhang

Wang

et al. 2022

Front. Nutr.

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In this study, soybeans during different germination stages were described and compared with regard to morphology, water content, protein, amino acids, and isoflavones. The optimal conditions for the hydrolysis of proteins obtained from germinated soybeans were determined using the response surface methodology. Gel filtration chromatography was used to separate germinated soybean protein hydrolysates after ultrafiltration, whereas 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ABTS•+, and FRAP assays were used to assess the antioxidant activity of different fractions. Findings of this study revealed that protein and isoflavone contents were high in soybean at 24 h following germination (the bud was about 0.5–1 cm). The proteins from germinated soybeans were hydrolyzed and separated into five fractions (G1–G5) and evaluated in terms of their molecular weight and antioxidant activity. Interestingly, the antioxidant activity was found to be higher in germinated soybean protein hydrolysates than in other soybean protein hydrolysates derived from soybean meal protein. This suggests that germination can effectively improve the utilization rate of soybean proteins. The antioxidant activity of G3 was best among G1–G5. The results obtained in this study demonstrate that germination for 24 h when the bud length is about 0.5–1 cm can be applied as a special pretreatment of plant seeds in the development of germinated foods. These findings can be used to identify the structure of the potential antioxidative hydrolysates for their possible exploitation in functional foods.

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“…Considering intestinal absorption, peptides, especially dipeptides, can be absorbed directly and faster into enterocytes than free amino acids (FAA) ( 4 ). Peptides derived from plant proteins could exhibit biological activity both in vitro and in vivo ( 5 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

“…Soybean peptides (SPs) can be obtained from in vitro enzymatic hydrolysis, microbial fermentation, and gastrointestinal digestion of soybean proteins ( 7 ). They usually comprised of 2–10 amino acid (AA) residues ( 8 ), and the percentage of soybean oligopeptides with molecular weight <1,000 Da is up to 89.16% when subjected to alcalase hydrolyzation ( 5 ). SPs have shown beneficial biological functions in addition to their nutritional value.…”

Section: Introductionmentioning

confidence: 99%

“…SPs have shown beneficial biological functions in addition to their nutritional value. At present, research involving SPs focuses primarily on their bioactive effects on human health, such as hypertension prevention ( 9 ), prebiotic effects on intestinal microbiota ( 10 , 11 ), intracellular antioxidant activity ( 12 ), anticancer activity ( 13 ), and immunomodulatory effects ( 5 , 14 ). The application of SPs as feed additives has emerged in livestock production, and their beneficial effects have been demonstrated in monogastric animals.…”

Section: Introductionmentioning

confidence: 99%

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In vitro and in vivo Studies of Soybean Peptides on Milk Production, Rumen Fermentation, Ruminal Bacterial Community, and Blood Parameters in Lactating Dairy Cows

Xie

Kong²,

Wang³

et al. 2022

Front. Vet. Sci.

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Soybean peptides (SPs), a feed additive derived from soybean, exhibit nutritional function and biological activity in monogastric animals, but limited studies have been conducted in dairy cows. Our experiments were conducted to evaluate the effects of SPs on the nutrient degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in vitro and milk production, rumen fermentation and bacterial community, and blood parameters of dairy cows. For in vitro experiment, ruminal fluids were collected from three ruminal cannulated Holstein dairy cows. A total of three levels of SPs (0, 0.38, and 1.92 g/kg DM of SPs) were added to the total mixed ration (TMR). Nutrient degradability and fermentation fluid pH were determined at 24 and 48 h using 3.0 g samples of the substrate. Gas production after 48 h was recorded by an automated trace gas recording system using 0.5 g samples of the substrate. The results showed that DM, NDF, ADF (p < 0.01), and CP (p < 0.05) degradabilities were significantly increased at 1.92 g/kg DM of SPs at 24 h, and asymptotic gas production (p = 0.05) was increased at 48 h. For in vivo experiment, 110 lactating Holstein cows (209.7 ± 65.2 DIM; 37.2 ± 6.4 kg/d milk yield) were randomly assigned to 0 (control group, CON) or 50 g/head/day SPs (SP-supplemented group). Yields of milk (p < 0.05), milk protein (p < 0.05), and milk lactose (0.05 < p < 0.10) increased on SPs supplementation; however, the milk fat percentage decreased (p < 0.05). The concentrations of individual volatile fatty acids (VFAs) (p < 0.05) and superoxide dismutase (SOD) (p < 0.01) were also increased. Rumen bacterial diversity in SP-supplemented cows was higher (p < 0.05). The relative abundances of Rikenellaceae_RC9_gut_group, Butyrivibrio, Selenomonas, and Shuttleworthia were significantly increased and that of Coprococcus was decreased (p < 0.05). Our results showed that supplementing 1.92 g/kg DM of SPs could improve the nutrient degradability in vitro and 50 g/head/day of SPs could improve milk production and antioxidant ability of dairy cows. The rumen bacterial diversity was also enhanced by SP supplementation.

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Structure identification of soybean peptides and their immunomodulatory activity

Cited by 44 publications

References 38 publications

Effects of ultra-high pressure treatment on angiotensin-converting enzyme (ACE) inhibitory activity, antioxidant activity, and physicochemical properties of milk fermented with Lactobacillus delbrueckii QS306

Effects of ultra-high pressure treatment on angiotensin-converting enzyme (ACE) inhibitory activity, antioxidant activity, and physicochemical properties of milk fermented with Lactobacillus delbrueckii QS306

Preparation and Antioxidant Properties of Germinated Soybean Protein Hydrolysates

In vitro and in vivo Studies of Soybean Peptides on Milk Production, Rumen Fermentation, Ruminal Bacterial Community, and Blood Parameters in Lactating Dairy Cows

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