Structure information from HPLC and on-line measured absorption spectra: flavones, flavonols and phenolic acids

Campos, Maria da Graça; Markham, Kenneth R.

doi:10.14195/978-989-26-0480-0

Cited by 63 publications

(78 citation statements)

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“…Phenolic compositions were obtained from a gradient method previously described (Campos and Markham, 2007), with a Perkin Elmer Series 200 HPLC system and a Perkin Elmer Brownlee Analytical C18 column (4.6 × 250 mm, 5 µm). Water adjusted to pH 2.5 with orthophosphoric acid was solvent A and acetonitrile was solvent B.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

“…Spectral data for all peaks were accumulated in the range of 200 to 400 nm using diode-array detection (Perkin Elmer Series 200). Structural information was obtained by comparisons of retention times (RT) and UV spectra of resolved compounds with those of reference compounds (apigenin: RT: 59.62, λ max : 269, 335; chlorogenic acid: RT: 28.16, λ max : 243sh, 293sh, 325), as well as with the principles of the UV theory developed by Campos and Markham (2007) for flavonoids and phenolic acids. The foliar phenol profile of each species was made up of all compounds present in the respective HPLC-DAD chromatogram, treating each compound as a single chemical character.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

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Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

et al. 2017

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The family Verbenaceae hosts important species used in traditional medicine of many countries. The taxonomic controversies concerning the specific delimitation of several of its species make it difficult to guarantee the botanical origin of herbal preparations based on species of this family. To contribute to the development of both specific chemomarkers and a quality control tool to authenticate the botanical origin of herbal preparations of Verbenacea species, we determined the foliar HPLC-DAD phenolic profiles and the antioxidant properties of 10 wild species of this family occurring in Mexico. The contents of phenols and flavonoids varied significantly among species. Priva mexicana showed the highest levels of total phenolics (53.4 mg g -1 dry tissue) and Verbena carolina had the highest levels of flavonoids (17.89 mg g -1 dry tissue). Relevant antioxidant properties revealed by antiradical and reducing power were found for the analyzed species. These properties varied significantly in a species-dependent manner. The phenolic compounds accumulated were flavones and phenolic acids. Flavones were the only type of flavonoids found. The results of a cluster analysis showed that the compounds were accumulated in species-specific profiles. The phenolic profiles are proposed as valuable chemomarkers that can become a useful tool for the quality control concerning the botanical origin of herbal medicinal preparations based on the species analyzed. In addition, phenolic profiles could contribute importantly to solve the taxonomic controversies concerning species delimitation in the family Verbenaceae.Keywords: Verbenacea, antioxidant activity, chemomarkers, flavones, phenolic profiles. Compostos fenólicos das folhas de dez especies selvagem de Verbenaceae como antioxidantes e quimiomarcadores específicos ResumoA família Verbenaceae compreende importantes espécies utilizadas na medicina popular de muitos países. As dificuldades taxonômicas relativas à delimitação específica de muitas das suas espécies face difícil a verificar a origem botânico das preparações herbales baseadas nas espécies desta família. Para fazer uma contribuição ao desenvolvimento de indicadores taxonômicos e dum método de controle de qualidade para verificar a origem botânico de preparações herbales das espécies de Verbenaceae, os perfis fenólicos, obtidos pares HPLC-DAD, e as atividades antioxidantes das folhas de 10 espécies selvagens Mexicanas desta família foram determinados. Os conteúdos dos compostos fenólicos totais e dos flavonoides foram significativamente diferentes entre as espécies. Priva mexicana apresentou a maior quantidade de compostos fenólicos totais (53.4 mg g -1 amostra seca) e Verbena carolina apresentou a maior quantidade de flavonoides (17.89 mg g -1 amostra seca). Verifica-se importantes propriedades antioxidantes, como os resultados dos ensaios da capacidade antiradical e do poder redutor indicaram. As propriedades antioxidantes foram significativamente diferentes entre as espécies. Verificou-se que os compostos fe...

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Section: Hplc-dad Analysismentioning

confidence: 99%

Section: Hplc-dad Analysismentioning

confidence: 99%

Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

et al. 2017

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“…To determine the flavonoid profiles, aliquots (100 µL) of each extract (concentrated to dryness and re-dissolved in 5 mL ethanol, as mentioned in the section Preparation of extracts) were analyzed as previously described (Campos & Markham, 2007) on a Perkin Elmer Series 200 HPLC system (Shelton, Connecticut, USA) and a Perkin Elmer Brownlee Analytical C18 column (4.6 × 250 mm, 5µm) (Shelton, Connecticut, USA), by an acidified acetonitrile-water gradient. Water adjusted to pH 2.5 with orthophosphoric acid was the solvent A, and acetonitrile was the solvent B, mixed according to the following gradient: starting with 100% A, decreasing to 91% over the next 12 min, to 87% over the next 8 min, to 67% over the next 12 min, to 57% over the next 10 min, and held at this level until the end of the 60 min analysis.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

“…Structural identification was obtained by direct comparisons of retention times and UV spectra of resolved compounds with those of standards; two kaempferol glycosides, identified as kaempferol-3,7-O-diglucoside (compound 2) and kaempferol-3-O[rhamnosyl-(1-6)-glucoside] (compound 6), and one quercetin glycoside, identified (Figures 1 and 2). The structural information of compounds, for which standards were not available, was obtained from their spectral parameters according to the compilations of Mabry, Markham, and Thomas (1970) and Campos and Markham (2007). Quantitative determinations were made by an external standard method, with the commercial reference (quercitrin), by area measurements, using the following standard curve: Area = −0.0046 + 0.0278 [Quercitrin], correlation coefficient r = 0.992.…”

Section: Hplc-dad Analysismentioning

confidence: 99%

Flavonoid composition and antioxidant capacity of the edible flowers ofAgave durangensis(Agavaceae)

Barriada-Bernal

Almaraz-Abarca

Delgado-Alvarado

et al. 2013

CyTA - Journal of Food

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The antioxidant properties and the flavonoid composition of extracts of different hydrophobicity prepared from the entire edible flowers of Agave durangensis were evaluated. Separately, total extracts of tepals and anthers-pollen were analyzed in the same manner. The high performance liquid chromatography with photodiode array detection (HPLC-DAD) analysis revealed a total of eight flavonols (five quercetin glycosides and three kaempferol glycosides), varying in number and concentration in the different extracts. The total extracts of the entire flowers showed the highest flavonoid content (1210.4 µg/g dry extract) and the most complex flavonoid profile (eight compounds). All the extracts showed important antioxidant activity, which was not evidently associated with their flavonoid content. The total extracts of tepals showed the highest antioxidant properties (total antioxidant capacity, free radical scavenging activity, and iron reducing capacity: 30.2 mg ascorbic acid equivalents, EC 50 = 0.074 µg/mL, and IC 50 = 43.28 µg/mL, respectively). The flowers of A. durangensis represent an important source of antioxidant flavonols.Keywords: Agave durangensis; edible flowers; flavonoid profiles; antioxidant capacity; flavonol glycosides Se evaluaron las propiedades antioxidantes y composición fenólica de extractos de diferente hidrofobicidad de flores comestibles enteras de Agave durangensis. Separadamente, se analizaron extractos crudos de tépalos y anteras-polen. El análisis de HPLC-DAD reveló en total ocho flavonoles (cinco glicósidos de quercetina y tres glicósidos de canferol), que variaron en número y concentración en los extractos. Los extractos totales de las flores enteras tuvieron el contenido más alto de flavonoides (1210,4 µg/g extracto seco) y el perfil de flavonoides más complejo (ocho compuestos). Todos los extractos mostraron importante actividad antioxidante, no claramente asociada al contenido de flavonoides. Los extractos crudos de tépalos mostraron las propiedades antioxidantes más altas (capacidad antioxidante total, capacidad bloqueadora de radicales libres, y capacidad reductora de fierro: 30,2 mg de equivalentes de ácido ascórbico, EC 50 = 0,074 µg/mL, e IC 50 = 43,28 µg/mL, respectivamente). Las flores de A. durangensis representan una fuente importante de flavonoles antioxidantes.

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“…Предполагаемую структуру остальных со-единений определяли сравнением УФ-и масс-спектров со спектрами известных соединений, приведенными в литературе (Mabry et al, 1970;Campos, Markham, 2007;Korul'kin et al, 2007) …”

Section: таблица 1 происхождение исследованных образцов Rhododendron unclassified

Ecological and geographical variation of morphometric parameters and flavonoid composition of Rhododendron parvifolium

2018

turczaninowia

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Ключевые слова: кластерный анализ, морфометрия, популяции, флавоноиды, Rhododendron parvifolium.Аннотация. Rhododendron parvifolium обладает обширным ареалом в Восточной Сибири и Дальнем Вос-токе России, включая остров Сахалин. Значительный полиморфизм морфологических и химических призна-ков вида слабо изучен. Нами проведено изучение морфологических показателей и состава флавоноидов в образцах растений из четырех популяций этого вида, в том числе растений из популяции, расположенной в Иркутской области (г. Слюдянка), отличающейся от остальных значительной высотой растений и размерами листьев. Климат в месте ее произрастания был значительно более мягким по сравнению с местообитаниями остальных популяций. Определяли длину и ширину листовой пластинки, длину черешка, листовой индекс -отношение ширины к длине листа. В результате кластерного анализа морфометрических данных выявлено обособление популяции из Иркутской области от остальных, образующих единый кластер. Методом высоко-эффективной жидкостной хроматографии в гидролизатах образцов удалось идентифицировать пять агликонов (дигидрокверцетин, мирицетин, кверцетин, лютеолин и кемпферол), главными из которых были кверцетин и ди-гидрокверцетин. Методом высокоэффективной жидкостной хроматографии-масс-спектрометрии в нативных экстрактах всех образцов идентифицированы гиперозид, авикулярин, а также пентозиды дигидрокверцетина и кверцетина, дезоксигексозид дигидрокверцетина, кверцетин и кемпферол в свободном виде. Основными гликозидными компонентами во всех образцах были пентозид дигидрокверцетина и гиперозид. Кластерный анализ данных по составу флавоноидов показал наличие двух кластеров, характеризующихся различными эдафическими условиями. Популяции, произрастающие на гольцах, отличались от растущих на болотах по-вышенным содержанием флавоноидов, в том числе гликозидов дигидрокверцетина, мирицетина, кверцетина, а также свободных агликонов.

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Structure information from HPLC and on-line measured absorption spectra: flavones, flavonols and phenolic acids

Cited by 63 publications

References 0 publications

Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

Foliar phenolic compounds of ten wild species of Verbenacea as antioxidants and specific chemomarkers

Flavonoid composition and antioxidant capacity of the edible flowers ofAgave durangensis(Agavaceae)

Ecological and geographical variation of morphometric parameters and flavonoid composition of Rhododendron parvifolium

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