Structure of a cyanobacterial gene encoding the 50S ribosomal protein L9

Malakhov, Michael P.; Wada, Hajime; Los, Dmitry A.; Sakamoto, Toshio; Murata, Norio

doi:10.1007/bf00027122

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…When we used a probe for transcription of the rplI gene, which is located upstream of the cytM gene [11], we detected only one transcript of 0.6 kb that corresponded to the rplI gene under normal conditions. When cells were exposed to the low temperature and the high‐intensity light, the level of the 0.6‐kb transcript increased 2.5‐fold and additional transcripts of 0.9 and 1.3 kb were also detected (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Isolation of RNA and Northern blotting analysis were carried out as previously described [11], with the exception that cell cultures were mixed with an equal volume of chilled 5% phenol in ethanol before harvest of cells by centrifugation. Probes for Northern blotting analysis, which covered the entire coding sequences of the corresponding genes, were amplified by polymerase chain reaction from plasmids that carried corresponding genes in the case of the rplI and cytM genes or from chromosomal DNA in the case of petE and petJ genes.…”

Section: Methodsmentioning

confidence: 99%

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Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis

1999

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The cytM gene for cytochrome c w was previously found in Synechocystis sp. PCC 6803. Northern blotting analysis revealed that the cytM gene was scarcely expressed under normal growth conditions but its expression was enhanced when cells were exposed to low temperature or high-intensity light. By contrast, the expression of the genes for cytochrome c T and plastocyanin was suppressed at low temperature or under highintensity light. These observations suggest that plastocyanin and/ or cytochrome c T , which are dominant under non-stressed conditions, are replaced by cytochrome c w under the stress conditions. z 1999 Federation of European Biochemical Societies.

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Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis

1999

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“…Rpl9 is one of the proteins from the large ribosomal subunit in prokaryotes; Rpl9 is known to bind directly to the 23S rRNA and belongs to a family of ribosomal proteins grouped on the basis of sequence similarities (Hoffman et al, 1994). The sequence of Synechocystis Rpl9 was characterized compared to that of Escherichia coli and chloroplasts of Arabidopsis and pea (Malakhov et al, 1993). The regulation of specific ribosomal protein expression by histidine kinase is uncertain and remains to be elucidated.…”

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confidence: 99%

Cyanobacterial hybrid kinase Sll0043 regulates phototaxis by suppressing pilin and twitching motility protein

Shin

Kang

et al. 2008

J Microbiol.

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The unicellular cyanobacterium Synechocystis sp. PCC 6803 glides toward a light source through the interplay of positive phototaxis genes and proteins. In genetic analysis, the complete disruption of the hybrid sensory kinase sll0043 produced negative phototaxis. Furthermore, Sll0043 was found to be a hub protein by in silico prediction of protein-protein interaction, in which Sll0043 was predominantly linked to seven two-component proteins with high confidence. To understand the regulation and networking of positive phototaxis proteins, the proteomic profile of the sll0043 mutant was compared to that of wild-type. In the sll0043 mutant, 18 spots corresponding to 15 unique proteins were altered by 1.3 to 59 fold; the spots were identified by 2-DE/MALDI-MS analysis. Down-regulated proteins in the sll0043 null-mutant included chaperonins, superoxide dismutase, and phycocyanin beta-subunit. In contrast, nine proteins involved in photosynthesis, translation, regulatory function, and other functions were up-regulated. In particular, a twitching motility protein (PilT1) was induced over 2-fold in sll0043 mutant. Moreover, semi-quantitative and quantitative RT-PCR analysis revealed that pilin (pilA1), pili motor (pilT1), and pili switch gene (pilT2) were significantly increased in sll0043 mutant. These results suggest that the hybrid kinase Sll0043 regulates positive phototaxis by suppressing the expression of pili biosynthesis and regulatory genes and through the interplay with positive phototaxis/motility two-component proteins.

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Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1

Sugita

Sugiura

1995

Molec. Gen. Genet.

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We isolated a 38 kDa ssDNA-binding protein from the unicellular cyanobacterium Synechococcus sp. strain PCC 6301 and determined its N-terminal amino acid sequence. A genomic clone encoding the 38 kDa protein was isolated by using a degenerate oligonucleotide probe based on the amino acid sequence. The nucleotide sequence and predicted amino acid sequence revealed that the 38 kDa protein is 306 amino acids long and homologous to the nuclear-encoded 370 amino acid chloroplast ribosomal protein CS1 of spinach (48% identity), therefore identifying it as ribosomal protein (r-protein) S1. Cyanobacterial and chloroplast S1 proteins differ in size from Escherichia coli r-protein S1 (557 amino acids). This provides an additional evidence that cyanobacteria are closely related to chloroplasts. The Synechococcus gene rps1 encoding S1 is located 1.1 kb downstream from psbB, which encodes the photosystem II P680 chlorophyll a apoprotein. An open reading frame encoding a potential protein of 168 amino acids is present between psbB and rps1 and its deduced amino acid sequence is similar to that of E. coli hypothetical 17.2 kDa protein. Northern blot analysis showed that rps1 is transcribed as a monocistronic mRNA.

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Structure of a cyanobacterial gene encoding the 50S ribosomal protein L9

Cited by 6 publications

References 28 publications

Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis

Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis

Cyanobacterial hybrid kinase Sll0043 regulates phototaxis by suppressing pilin and twitching motility protein

Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1

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Structure of a cyanobacterial gene encoding the 50S ribosomal protein L9

Cited by 6 publications

References 28 publications

Balanced regulation of expression of the gene for cytochrome cM and that of genes for plastocyanin and cytochrome c6 in Synechocystis

Balanced regulation of expression of the gene for cytochrome cM and that of genes for plastocyanin and cytochrome c6 in Synechocystis

Cyanobacterial hybrid kinase Sll0043 regulates phototaxis by suppressing pilin and twitching motility protein

Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1

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Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis

Balanced regulation of expression of the gene for cytochrome c_M and that of genes for plastocyanin and cytochrome c₆ in Synechocystis