Structure of a novel exopolysaccharide produced by Burkholderia vietnamiensis, a cystic fibrosis opportunistic pathogen

Cescutti, Paola; Cuzzi, Bruno; Herasimenka, Yury; Rizzo, Roberto

doi:10.1016/j.carbpol.2013.01.047

Cited by 22 publications

(6 citation statements)

References 27 publications

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“…Burkholderia spp. in biofilms were shown to produce multiple extracellular polysaccharides (e.g., Bep, cellulose and cepacian) and protein-based matrix components (e.g., BapA, fimbria, lectins) (Ferreira et al, 2011; Yakandawala et al, 2011; Fazli et al, 2012; Inhulsen et al, 2012; Cescutti et al, 2013). A correlation between colony morphology and the production of extracellular matrix components has been reported for several bacterial species, including B. cenocepacia (e.g., Fazli et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

et al. 2019

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Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. cenocepacia are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of B. cenocepacia H111 with respect to motility, colony morphology, pellicle formation, biofilm formation, and virulence. We found that RpfR is a key regulator of c-di-GMP signaling in B. cenocepacia, affecting a broad spectrum of phenotypes under various environmental conditions. In addition, we identified Bcal2449 as a regulator of B. cenocepacia virulence in Galleria mellonella larvae. While Bcal2449 consists of protein domains that may catalyze both c-di-GMP synthesis and degradation, only the latter was essential for larvae killing, suggesting that a decreased c-di-GMP level mediated by the Bcal2449 protein is required for virulence of B. cenocepacia. Finally, our work suggests that some individual proteins play a role in regulating exclusively motility (CdpA), biofilm formation (Bcam1160) or both (Bcam2836).

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Section: Resultsmentioning

confidence: 99%

Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

et al. 2019

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“…The structural analysis of LA-EPS-20079 was performed using the ‘determine structure’ module of the CASPER program Jansson et al [ 18 ]; Lundborg and Widmalm [ 19 ] and Rönnols et al [ 20 ], suggesting the following structure residues; three d -GlcAp, one l -fucose and one d -Glc residues. Furthermore, previously published 1 H-and 13 C-NMR data by Dutton and Merrifield [ 21 ]; Cescutti et al [ 22 ]; Wang and Yang [ 23 ]; Perry et al [ 24 ] and Fotakis and Timbrell [ 25 ], together, with our current complete assignment of 1 H and 13 C chemical shifts and the inter-residue connectivity, of the monosaccharides residues of LA-EPS-20079 allowed LA-EPS-20079 to be formulated as α- d -Glc(1→2)][α- l -Fuc(1→4)] α- d -GlcA(1→2) α- d -GlcA(1→2) α- d -GlcA.…”

Section: Discussionmentioning

confidence: 99%

A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer

et al. 2018

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BackgroundThe direct link between inflammatory bowel diseases and colorectal cancer is well documented. Previous studies have reported that some lactic acid bacterial strains could inhibit colon cancer progression however; the exact molecules involved have not yet been identified. So, in the current study, we illustrated the tumor suppressive effects of the newly identified Lactobacillus acidophilus DSMZ 20079 cell-free pentasaccharide against colon cancer cells. The chemical structure of the purified pentasaccharide was investigated by MALDI-TOF mass spectrum, 1D and 2D Nuclear Magnetic Resonance (NMR). The anticancer potentiality of the purified pentasaccharide against both Human colon cancer (CaCo-2) and Human breast cancer (MCF7) cell lines with its safety usage pattern were evaluated using cytotoxicity, annexin V quantification and BrdU incorporation assays. Also, the immunomodulatory effects of the identified compound were quantified on both LPS-induced PBMC cell model and cancer cells with monitoring the immunophenotyping of T and dendritic cell surface marker. At molecular level, the alteration in gene expression of both inflammatory and apoptotic pathways were quantified upon pentasaccharide-cellular treatment by RTqPCR.ResultsThe obtained data of the spectroscopic analysis, confirmed the structure of the newly extracted pentasaccharide; (LA-EPS-20079) to be: α-d-Glc (1→2)][α-l-Fuc(1→4)] α-d-GlcA(1→2) α-d-GlcA(1→2) α-d-GlcA. This pentasaccharide, recorded safe dose on normal mammalian cells ranged from 2 to 5 mg/ml with cancer cells selectivity index, ranged of 1.96–51.3. Upon CaCo-2 cell treatment with the non-toxic dose of LA-EPS-20079, the inhibition percentage in CaCo-2 cellular viability, reached 80.65 with an increase in the ratio of the apoptotic cells in sub-G0/G1 cell cycle phase. Also, this pentasaccharide showed potentialities to up-regulate the expression of IKbα, P53 and TGF genes.ConclusionThe anticancer potentialities of LA-EPS-20079 oligosaccharides against human colon cancer represented through its regulatory effects on both apoptotic and NF-κB inflammatory pathways.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0877-z) contains supplementary material, which is available to authorized users.

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“…This protective effect was also provided by scavenging, resulting in extensive deacetylation of cepacian followed by the degradation of the polysaccharide backbone [5]. Although cepacian is the most common EPS produced by Bcc species, the production of at least six other polysaccharides, either synthesized alone or in combination with cepacian, has been demonstrated [6]. Burkholderia vietnamiensis LMG 10929, used in this study, has been shown not to produce cepacian as main EPS but a polysaccharide containing a fucose residue in its backbone.…”

Section: Introductionmentioning

confidence: 99%

Investigating the role of matrix components in protection of Burkholderia cepacia complex biofilms against tobramycin

Messiaen

Nelis

Coenye

2014

Journal of Cystic Fibrosis

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Structure of a novel exopolysaccharide produced by Burkholderia vietnamiensis, a cystic fibrosis opportunistic pathogen

Cited by 22 publications

References 27 publications

Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia

A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer

Investigating the role of matrix components in protection of Burkholderia cepacia complex biofilms against tobramycin

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