Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice

Cai, Gaofeng; Wu, Caihong; Zhu, Tianyu; Song, Peng; Xu, Shuwen; Hu, Yuanliang; Liu, Zhenguang; Yang, Yang; Wang, Deyun

doi:10.1016/j.ijbiomac.2023.123386

Cited by 26 publications

(3 citation statements)

References 37 publications

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“…The experimental results showed that the absorbance value of DDO group was significant than control group, which indicated that DDO could significantly promote the proliferation of lymphocytes in vitro and in vivo, and the medium dose possessed the best efficacy in vivo. Many studies have proved that traditional Chinese medicine and its ingredients and compounds had the better effect on promoting lymphocyte proliferation [ 26 , 27 ]…”

Section: Discussionmentioning

confidence: 99%

Study on the material basis and immunological enhancement activity of dangdi oral liquid

Zhou,

Liu,

Zhao

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Study on the material basis and immunological enhancement activity of dangdi oral liquid

Zhou,

Liu,

Zhao

et al. 2024

Heliyon

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“…The impact on both T and B lymphocytes was assessed following the methodology of previous research, with minor modifications [24]. In brief, RPMI 1640 medium (containing 10% FBS and 1% P/S) was used to adjust the concentration of mouse spleen lymphocytes to 1 × 10 6 cells/mL, then seeded at 100 µL per well in a 96-well plate and cultured at 37 • C, 5% CO 2 for 24 h. Subsequently, different concentrations (20-250 µg/mL) of PPRP (90 µL) and either ConA (for T lymphocytes) or LPS (for B lymphocytes) (10 µL, 10 µg/mL) were added to the respective wells, and the cells were incubated for an additional 24 h. Post-incubation, 20 µL of CCK-8 was added to each well, and proliferation activity was determined using a microplate reader (SynergyMx, BioTek) at 540 nm, following the instructions of the assay kit.…”

Section: Specific Immune Responsesmentioning

confidence: 99%

Structural Characterization and Immunological Activity of Polysaccharide Degradation Products from Phlebopus portentosus

Yu,

Cai,

Wang

et al. 2024

Separations

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Phlebopus portentosus is an edible and medicinal mushroom with a delicious taste and high nutritional value. The oligosaccharides derived from P. portentosus may be the material basis for its biological activity. The degradation of polysaccharide and the maintenance of its activity after degradation are key steps in related research. This study applied an acid degradation method to prepare P. portentosus refined polysaccharide (PPRP) with a smaller molecular weight, and the optimal hydrolysis conditions determined were a temperature of 80 °C, an acid concentration of 2 mol/L, and a hydrolysis time of 2 h. The polysaccharide structure and immune activity were then further investigated. The results showed that the PPRP comprised two fractions with approximate weights of 61,600 Da and 5500 Da. The monosaccharide composition of PPRP was mannose, rhamnose, glucose, and galactose, with a molar ratio of 1.00: 22.24: 2.93: 1.03. The major functional groups included O-H, C-H, C-O, and C-O-C. The glycosidic bond types were mainly α- and β-glycosidic bonds. Cell experiments demonstrated that PPRP could significantly increase the proliferation of macrophages and enhance the cytotoxicity of NK cells. Moreover, PPRP also significantly promoted the proliferation of B lymphocytes and T lymphocytes, especially at a concentration of 200 μg/mL. This study furnishes scientific evidence underlining the significant potential of PPRP in immune activity, thereby serving as a material basis and scientific bedrock for further investigations into the mechanism of P. portentosus oligosaccharide activity.

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“…The authors identified a pro-inflammatory profile, where phagocytosis rate, NO concentration, and IL-1b, IL-6, and TNF-a levels were increased after treatment in a dose-response manner [35]. Pueraria lobata, a known herbal medicine with α-glucan polysaccharides, induced IL-2, IL-4, and TNF-a increase, enhancing specific T and B cell populations in vitro [36]. This backbone of α(1,4) glucans has been described as having proinflammatory properties in sources other than plants, such as clams and fungi [37,38].…”

Section: Immunomodulationmentioning

confidence: 99%

Polysaccharides from Medicinal Plants: Bridging Ancestral Knowledge with Contemporary Science

Pedrosa,

Fabi

2024

Plants

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Plants are a core part of cultural identity, as part of a diet, decorations, ceremonies, or as medicinal agents. Empirical knowledge regarding plants and their healing potential has existed worldwide for centuries. With the advance of science and technology, not only is the refinement of such sources or isolation of specific compounds possible, but these compounds can also be characterized based on their natural occurrence. Besides their importance for plant metabolism and structure, polysaccharides have been demonstrated to have substantial positive human health impacts on inflammation, metabolism, oxidative stress, and others. As an inherent part of plant cell walls, many polysaccharides from medicinal herbs, such as fructans, glucans, and pectins, have been extracted and analyzed for their structure and function. However, a review summarizing a significant portion of these studies was still unavailable. This review helps to fill the knowledge gap between polysaccharide bioactivity, their structure, and their plant matrix sources, focusing on historical medicinal usage.

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Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice

Cited by 26 publications

References 37 publications

Study on the material basis and immunological enhancement activity of dangdi oral liquid

Study on the material basis and immunological enhancement activity of dangdi oral liquid

Structural Characterization and Immunological Activity of Polysaccharide Degradation Products from Phlebopus portentosus

Polysaccharides from Medicinal Plants: Bridging Ancestral Knowledge with Contemporary Science

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