Structure of an Acidic Polysaccharide Elaborated by<i>Acetobacter</i>sp. NBI 1005

Tayama, Kenji; Minakami, Hiroyuki; Fujiyama, Seiichi; Masai, Hiroshi; Misaki, Akira

doi:10.1080/00021369.1986.10867547

Cited by 5 publications

(8 citation statements)

References 3 publications

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“…Another source of possible substrates were A . xylinum strains forming polymers which are thought to show some resemblance to part of the xanthan structure (Tayama et al, 1986). No detectable TBA-positive material was found from any of the preparations tested, after prolonged exposure to the xanthan lyases.…”

Section: Production and Purijication Of Xanthan Lyasesmentioning

confidence: 93%

“…composed of the characteristic pentasaccharide repeating unit, with or without associated acyl groups. Certain Acetobacter xylinurn polysaccharides have been reported to contain structural similarities to xanthan (Tayama et al, 1985(Tayama et al, , 1986, but no enzyme activity on these was detected. Activity was also absent if the xanthan molecule had been modified by various chemical procedures.…”

Section: Production and Purijication Of Xanthan Lyasesmentioning

confidence: 99%

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Xanthan Lyases - Novel Enzymes Found in Various Bacterial Species

Sutherland¹

1987

Microbiology

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Xanthan lyases, cleaving the terminal P-mannosidic linkage of the side-chain of the exopolysaccharide xanthan from Xanthomonas campestris, have been obtained from several sources. These include a Bacillus species, a Corynebacterium species and a mixed culture. The lyases were initially associated with endo-P-glucanases cleaving the main chain of xanthan. Partial purification of the enzymes was achieved and the Bacillus preparation was separated by FPLC into material free of endoglucanase and glycosidase activities. The lyase was active on polysaccharides with and without acetate and pyruvate. The optimal size of the substrate appeared to be in the range of degree of polymerization (DP) 25-35, i.e. 5-7 repeat units of the polysaccharide. No activity was found against xanthan modified by reduction of the carboxyl groups or by the addition of amine or hydroxyethyl groups. The combined action of the lyase and the endoglucanase yielded a series of oligosaccharides, each with a side-chain terminating in an unsaturated uronic acid and containing the molar ratio of D-glucose to D-mannose, 2 : 1.

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Section: Production and Purijication Of Xanthan Lyasesmentioning

confidence: 93%

Section: Production and Purijication Of Xanthan Lyasesmentioning

confidence: 99%

Xanthan Lyases - Novel Enzymes Found in Various Bacterial Species

Sutherland¹

1987

Microbiology

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“…Yet, in previous studies, Soumahoro et al (2015) demonstrated that all tested strains are able to growth at 45 °C and strains 49 D, 139 D and 56 AB at 50 °C. On the other hand, Saeki et al (1997) demonstrated the increase in stability with increase of temperature (above 50 °C) of enzymes notably alcohol dehydrogenase and aldehyde dehydrogenase implicated in conversion of ethanol into acetic acid (Tayama et al, 1989). Therefore, incapacity of these strains to produce acetic acid at 45 °C is probably due to several factors unexplained in our knowledge especially since Moghadami et al (2013) have demonstrated that thermotolerant strains have high ethanol oxidation rate.…”

Section: Resultsmentioning

confidence: 98%

Effects of culture conditions on acetic acid production by bacteria isolated from Ivoirian fermenting cocoa (<i>Theobroma cacao</i> L.) beans

Soumahoro¹,

Goualié²,

Adom³

et al. 2016

J. App. Bioscience.

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Objectives: This study investigates the acidification capacity under various culture conditions of high acetic acid producer AAB strains previously isolated from Ivoirian cocoa beans fermentation. Methodology and Results: Effect of culture conditions was studied in agar medium and acid production was monitored by measuring the clear halo diameter during incubation. All tested strains showed acetic acid production at 30, 35 and 40 °C. Moreover, at initial concentration 0.1-0.4 %, lactic and citric acids stimulated acidification capacity of these strains with increase rate ranged from 50 to 100 % while acetic acid reduced this capacity. In addition, maximum acetic acid production capacity was obtained for strains 123 D; 56 AB and 49 D at 8 % ethanol initial concentration. Conclusions and application of findings: This study shows that all tested strains are able to produce acetic acid under certain culture conditions similar to cocoa fermentation stress. However, cocoa fermentation assay is needed to better estimate the performance of selected strains.

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“…This has been extensively studied in lactic acid bacteria [ 60 ]. Only limited data exist for acetan AC-1 purified from Komagataeibacter xylinus NBI 1005 [ 31 ]. Acetan AC-1 induces production of interleukin-12 p40 and tumor necrosis factor-α by macrophages and has in vitro effect by regulating Th2-mediated allergic response.…”

Section: Potential Applications Of Acetan In Industrymentioning

confidence: 99%

Acetan and Acetan-Like Polysaccharides: Genetics, Biosynthesis, Structure, and Viscoelasticity

et al. 2021

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Bacteria produce a variety of multifunctional polysaccharides, including structural, intracellular, and extracellular polysaccharides. They are attractive for the industrial sector due to their natural origin, sustainability, biodegradability, low toxicity, stability, unique viscoelastic properties, stable cost, and supply. When incorporated into different matrices, they may control emulsification, stabilization, crystallization, water release, and encapsulation. Acetan is an important extracellular water-soluble polysaccharide produced mainly by bacterial species of the genera Komagataeibacter and Acetobacter. Since its original description in Komagataeibacter xylinus, acetan-like polysaccharides have also been described in other species of acetic acid bacteria. Our knowledge on chemical composition of different acetan-like polysaccharides, their viscoelasticity, and the genetic basis for their production has expanded during the last years. Here, we review data on acetan biosynthesis, its molecular structure, genetic organization, and mechanical properties. In addition, we have performed an extended bioinformatic analysis on acetan-like polysaccharide genetic clusters in the genomes of Komagataeibacter and Acetobacter species. The analysis revealed for the first time a second acetan-like polysaccharide genetic cluster, that is widespread in both genera. All species of the Komagataeibacter possess at least one acetan genetic cluster, while it is present in only one third of the Acetobacter species surveyed.

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Structure of an Acidic Polysaccharide Elaborated byAcetobactersp. NBI 1005

Cited by 5 publications

References 3 publications

Xanthan Lyases - Novel Enzymes Found in Various Bacterial Species

Xanthan Lyases - Novel Enzymes Found in Various Bacterial Species

Effects of culture conditions on acetic acid production by bacteria isolated from Ivoirian fermenting cocoa (<i>Theobroma cacao</i> L.) beans

Acetan and Acetan-Like Polysaccharides: Genetics, Biosynthesis, Structure, and Viscoelasticity

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